Dr Dirk Opperman
Associate Professor
Microbial Biochemical and Food Biotechnology
IB 61
051 4012714

Short CV

2008 PhD Biochemistry UFS
2008-2009 Post Doctoral Researcher Max Planck Institute (KOFO) Germany
2010-2011 Lecturer Biochemistry UFS

2011-Present Senior Lecturer Biochemistry UFS


Publications (Short List)

Publications (Since 2010)

23.    Opperman, D.J. (2017) Structural investigation into the C-terminal extension of the ene-reductase from Ralstonia (Cupriavidus) metallidurans. Proteins: Structure, Function and Bioinformatics 85:2252-2257.

22.    Mthethwa, K.S., Kassier, K., Engel, J., Kara, S., Smit, M.S. and Opperman D.J. (2017) Fungal BVMOs as alternatives to cyclohexanone monooxygenase. Enz. Microbial. Technol. 106:11-17. 

21.    Ferroni, F.M., Tolmie, C., Smit, M.S. and Opperman, D.J. (2017) Alkyl formate ester synthesis by a fungal Baeyer-Villiger monooxygenase. ChemBioChem 18:515-517.

20.    Tosstorff, A., Kroner, C., Opperman, D.J., Hollmann, F. and Holtmann, D. (2017) Towards electro enzymatic processes involving old yellow enzymes and mediated cofactor regeneration. Eng. Life Sci. 17:71-76.

19.    Ferroni, F.M., Tolmie, C., Smit, M.S. and Opperman, D.J. (2016) Structural and catalytic characterization of a fungal Baeyer-Villiger monooxygenase. PloS one 11:e0160186.

18.    Kara, S., Schrittwieser, J.H., Gargiulo, S., Ni, Y., Yanase, H., Opperman, D.J., van Berkel, W.J.H. and Hollmann, F. (2015) Complete enzymatic oxidation of methanol to carbon dioxide: Towards more eco-efficient regeneration systems for reduced nicotinamide cofactors. Adv. Synth. Catal 357:1687-1691. (IF 5.66)

17.    Pennec, A., Hollmann, F., Smit., M.S. and Opperman, D.J. (2015) One-pot conversion of cycloalkanes to lactones. ChemCatChem. 7:236-239. (IF 4.56)

16.    Pennec, A., Jacobs, C.L., Opperman, D.J. and Smit, M.S. (2015) Revisiting cytochrome P450 mediated oxyfunctionalization of linear and cyclic alkanes. Adv. Synth. Catal. 357:118-130. (IF 5.66).

15.     Holtmann, D., Fraaije, M.W. Arends, I.W.C.E., Opperman, D.J. and Hollmann, F. (2014) The taming of oxygen: Biocatalytic oxyfunctionalizations. Chem. Commun. 50:13180-13200. (IF 6.38)

14.     Ferroni, F.M., Smit, M.S. and Opperman, D.J. (2014) Functional divergence between closely related Baeyer-Villiger monooxygenases from Aspergillus flavus. (2014). J. Mol. Catal. B. Enzym. 107:47-54. (IF 2.82)

13.     Litthauer, S., Gargiulo, S., van Heerden, E., Hollmann, F., Opperman, D.J. (2014) Heterologous expression and characterization of the Ene-reductases from Deinococcus radiodurans and Ralstonia metallidurans. J. Mol. Catal. B. Enzym. 99:89-95. (IF 2.82)

12.     Onstott, T.C., Magnabosco C., Aubrey, A.D., Burton, A.S., Dworkin, J.P., Elsila, E., Grunsfeld, S., Cao, B.H., Hein, J.E., Glavin, D.P., Kieft, T.L., Silver, B.J., Phelps, T.J., van Heerden, E., Opperman, D.J. and Bada, J.L. (2014) Does aspartic acid racemization constrain the depth limit of the subsurface biosphere? Geobiology, 12:1-19. (IF 3.04)

11.     Paul, C.E., Gargioulo, S, Opperman, D.J., Lavandera, I, Gotor-Fernández, V., Gotor, V., Taglieber, A., Arends, I.W.C.E and Hollmann F. (2013) Mimicking nature: Synthetic nicotinamide cofactors for C=C bioreduction using enoate reductases. Org. Lett. 15:180-183. (IF 6.14)

10.     Kille, S., Acevedo-Rocha, C.G., Parra, L.P., Zhang, Z-G., Opperman, D.J., Reetz, M.T. and Acevedo, J.P. (2013) Reducing codon redundancy and screening effort of combinatorial protein libraries created by saturation mutagenesis. ACS Synth. Biol. 2:83-92.

9.     Gargiulo, S., Opperman, D.J., Hanefeld, U., Arends, I.W.C.E and Hollmann, F. (2012) A biocatalytic redox isomerisation. Chem. Commun., 48:6630-6632. (IF 6.38)

8.     Gudiminchi, R.K., Randall, C., Opperman, D.J., Olaofe, O., Harrison, S.T.L., Albertyn, J. and Smit, M.S. (2012).Whole-cell hydroxylation of n-octane by Escherichia coli strains expressing the CYP153A6 operon. Appl Microbiol Biotechnol. 96:1507-1516. (IF 3.28)

7.     Bernard J., van Heerden, E., Arends, I.W.C.E., Opperman, D.J. and Hollmann, F. (2012) Chemoenzymatic reduction of conjugated C=C double bonds. ChemCatChem, 4:196-199. (IF 5.18)

6.     Opperman, D.J. and Reetz, M.T. (2010) Towards Practical Baeyer-Villiger monooxygenases: Design of cyclohexanone monooxygenase with enhanced oxidative stability. ChemBioChem, 11:2589-2596. (IF 3.74)

5.     Opperman, D.J., Sewell, B.T., Litthauer, D., Isupov, M.N., Littlechild, J.A. and van Heerden, E. (2010) Crystal structure of a thermostable old yellow enzyme from Thermus scotoductus SA-01. BBRC 393:426-431. (IF 2.548)



    Structure-Function and Evolutionary Relationships of Baeyer-Villiger monooxygenases
    Collaboration Dr S Kara (TU Hamburg-Harburg - Germany)
    Funding National Research Foundation (NRF) SA

    Enzymatic cascade reactions
    Collaboration Prof MS Smit (UFS – SA) and Dr F Hollmann (TU Delft – Netherlands)
    Funding Technology and Innovation Agency (TIA) SA 

    X-ray structure determination of cytochrome P450s
    Collaboration Prof MS Smit (UFS – SA) 

    Structure-Function of Ene-Reductases from Directed Evolution studies
    Collaboration Dr F Hollmann (TUDelft –Netherlands) and Dr S Höbenreich (Philipps-Universität Marburg - Germany)

    Area(s) of Interest

    Protein Structure / X-ray crystallography
    Directed Evolution

    Courses Presented

    Protein Biochemistry (BOCP3724)

    BSc Hons General Techniques Course

    BSc Biochemistry Hons. Advanced Protein Structure and Function (BOCE6844)


Faculty Manager: Ms Lee-Ann Frazenburg
T: +27 51 401 3199
E: damonsle@ufs.ac.za

Marketing Manager: Mrs Elfrieda Lötter
T: +27 51 401 2531
E: lottere@ufs.ac.za

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