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30 October 2020 | Story Leonie Bolleurs

The Department of Science and Technology has extended two of the National Research Foundation’s SARChI research chairs at the University of the Free State (UFS). 

The Research Chair in Diseases and Quality of Field Crops, together with the Research Chair in Vector-borne and Zoonotic Pathogens, have both been extended for another five years. 

Prof Maryke Labuschagne, currently Professor of Plant Breeding in the Department of Plant Sciences, is leading the chair on Diseases and Quality of Field Crops.

The Chair on Vector-borne and Zoonotic Pathogens is headed by Prof Felicity Burt from the Division of Virology in the Faculty of Health Sciences.

Prof Corli Witthuhn, Vice-Rector: Research, says it was the hard work and commitment of Profs Labuschagne and Burt that resulted in the extension of the SARChI research chairs. “They have excelled in terms of student supervision and publications in high-impact international journals.  They also serve as mentors for young academics, postdoctoral fellows, and colleagues through their passion for their different fields of interest.”

Prof Witthuhn believes that this extension of the two SARChI chairs speaks of the progress that the UFS has made in terms of developing itself as a research-led university. “We are proud of the two senior academics for their supervision, mentorship, and leadership and their contribution to building our reputation,” she says. 

Diseases and Quality of Field Crops

The focus of the research chair in Diseases and Quality of Field Crops is on advancing food security and nutrition in Africa and contributing to poverty reduction and achieving sustainability goals. 

Prof Labuschagne says despite recent advances, the headlines regarding hunger and food security remain alarming: one in nine people on earth will go to bed hungry every night. Globally, 800 million people do not have enough to eat to be healthy, and a third of all deaths among children under five in developing countries are linked to undernourishment. 

She believes the uniqueness and strength of the research chair lies in a two-pronged approach, namely the breeding of cereal crops for resistance to fungal diseases, and improving the quality of crops for processing and consumption, thus making an impact on food security in South Africa and the rest of Africa through this collaborative effort. 

She is confident that the extension of the research chair will allow them to continue and to expand their research, “which has built up a lot of momentum”.

Besides the 12 PhD and 8 MSc degrees they delivered in the first five years, they also contributed significant research outputs and cultivar releases. She adds that they would like to expand on the significant international collaboration they have established. 

Vector-borne and Zoonotic Pathogens

According to Prof Burt, the SARChI chair in Vector-borne and Zoonotic Pathogens builds on existing research strengths at the UFS and aims to contribute towards identifying and investigating medically significant arboviruses and zoonotic viruses in the country.
 
“To date, the research chair has facilitated progress towards establishing serosurveillance studies for various vector-borne viruses, specifically Crimean-Congo haemorrhagic fever virus, a tick-borne and zoonotic virus that causes severe disease with fatalities.”

The team of researchers operating within this research chair is currently also performing studies to determine the seroprevalence of severe acute respiratory coronavirus 2 (SARS-CoV-2) in the Free State.

Prof Burt has always taken the importance of community engagement into account, and with the current pandemic, she believes that it is now more important than ever to increase public awareness of zoonotic diseases.

She emphasises that the majority of new and emerging viruses are zoonotic in origin and that the current SARS-CoV-2 pandemic highlights the impact of an emerging zoonotic pathogen on society. Therefore, she feels that it is important to build capacity in this field and to focus research efforts on identifying and understanding where these pathogens cycle in nature, the potential for spill-over to humans, and what the drivers are for the emergence of these pathogens.

Prof Burt trusts that the renewal of the research chair will allow them to take advantage of the new biosafety laboratory that the UFS has invested in. “This will permit us to research pathogens that were previously excluded from our programme due to biosafety considerations.  The chair will furthermore contribute towards enhancing, strengthening, and developing research and knowledge in the field of epidemiology and pathogenesis of vector-borne and zoonotic viruses,” she says. 

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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