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12 December 2018 | Story Mothepane Lebopo

The door closed. My eyes opened.

My dreams were halted as I sat up. She was already outside my window, the midnight moonlight lit her skin and erasing my fingertips on her arms. I opened the window… cold truth blew in. It stung my heart. She was going.

“Seriously? After four months this is how you are going to leave?”

Silence.

She was trying to control her breathing, to keep it as flat as possible. She had a unique, annoying gift of being able to compose herself in such situations, especially when she knew it was needed.

She stared at me.

My heart was pounding against my chest. In anger. In desperation. It had settled on her, but clearly she wouldn’t let me get close to hers.

I felt the first tear roll down my cheek. I quickly wiped away the second one. She just stared…

She could have been looking at me, thinking of other things. With her you never knew. She turned.

“Wait, please wait. Did you ever love me?”

She stuck her tongue out and left.

And I knew that was it: we were over. Thinking back, I might have known for a while that it was coming. But still… being prepared for something doesn’t guarantee your heart won’t break when it actually happens.

I left the window open, slightly. My head was spinning and my heart was tearing.

I laid on what was supposed to be our bed and dug my head in a pillow in an attempt to block out reality. It was useless; warm liquid from my broken heart poured out through my eyes. All I could smell was her.

But what was I expecting? It could never work. We were two puzzle pieces from different sets. Two pieces that were never supposed to fit… We tried to force it, and it ended in pain.

She was such an odd person. She had this ‘forbidden love’ thing about her. Being hers was strange, I knew she wasn't mine but I still tumbled head over heels. Being with her was like cheating on a diet. Or texting when you’re supposed to study.

She had beautiful, wild eyes that had perhaps seen too much. She got high on other people’s vulnerability. When her arms locked around me, she wasn’t just holding me, she was searching for pain. Insecurity. She would pin me down and kiss my nose. When she felt my guard coming up, she would tickle me and my power would leave me and enter her. She always won.

Often we’d try to watch the stars. I could never concentrate, her beauty was fierce and demanded undivided attention. She couldn’t focus either. She looked at the stars, not for their beauty, but for adventure. She looked at them as a guide.

I felt her hot blood in her embrace, she had to move to keep cool. There was rarely a still moment. Always dancing. Always moving.

I guess that’s what attracted me to her. I made her my adventure. I wanted to see what she had seen. I told her I was happy where I was but in reality I wanted to go everywhere she went. Wherever the stars would take her.

My lips only met hers when she was drunk. Perhaps she didn’t want to remember showing a little bit of emotion, being a bit vulnerable in front of me. But even then she rarely shared her thoughts with me.

So her secrets are still with her, while she knows mine.

That wild girl, may I never hold her again. She said she didn’t like it. She wanted to feel liberated. And my arms didn’t offer her that.

The girl with a storm in her heart had started a fire in mine and left.

I look out the window, where she had been standing. I almost smiled. What was I thinking?  Thinking I could fix her? Whether I love her or hate her, it makes no difference because she’s not here. She’s not coming back.

I will never know what exactly she wanted with me. But I’ll grow wiser from this.

You can’t teach someone who’s power hungry to surrender. You can’t mould someone who despises being held. You can’t put out a wild fire. Don’t try to pick wild flowers, because their thorns will pierce your skin and then they will wither because of your blood. But their scent will linger forever.

Now I know. You can’t tame someone who is wild. You shouldn’t offer your heart to someone who has sold her soul to adventure.

Don’t try to love someone who can’t be still.

 

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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