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19 July 2021 | Story Lunga Luthuli | Photo Supplied
Fletcher Hiten, Chief Bioanalyst at FARMOVS, next to Aurora.

The Bioanalytical Services Division (BASD) at FARMOVS comprises a group of skilled and passionate scientists involved in the quantification of drugs, metabolites, and biomarkers in various biological matrices. One of their Analytical Science experts, Fletcher Hiten, explains what sets their team apart from the rest.

“Over the past 47 years, we have developed almost 600 validated assay methods. Most of these methods are for the analysis of ‘small’ molecules using chromatographic techniques such as LC-MS/MS, GC-MS, and HPLC, although LC-MS/MS is the technique of choice. New bioanalytical assays are continuously being development and validated in adherence to international regulatory guidelines set by the US-FDA and European Medicines Agency (EMA),” says Hiten.

“Recently, we decided to enhance our capabilities by recruiting exceptional talent. The newest member of the FARMOVS team is Aurora, a SCIEX Triple Quad™ 7500 LC-MS/MS mass analyser. Aurora is Latin for ‘dawn’: the beginning of a new era, especially one considered favourable. The SCIEX 7500 is currently marketed as the most sensitive triple quadrupole mass spectrometer available, allowing for sub-picogram/ml quantification. This means that Aurora will set FARMOVS apart from other clinical research organisations (CROs), creating an exciting and favourable landscape for clients to explore new partners in research.” 

Hiten stated: “If there was ever a time to move your next study to FARMOVS, it is now. To have Aurora on our team has many advantages, given that our clients can access unprecedented analytical sensitivity, which enables the quantification of pharmacokinetic (PK) profiles of drugs that have very low systemic absorption. These include predominantly local acting drugs, such as plasma concentrations of respiratory drugs (e.g., tiotropium and ipratropium), topically applied creams and ointments, and ophthalmology drops with ultra-sensitivity.”

“In addition, the quantification of drugs in low-volume matrices will also be exponentially enhanced, enabling the quantification of body fluids, where only a few microlitres can be collected, for example vaginal fluid, dried blood spots, cerebrospinal fluid, aqueous humour, synovial fluid, and epidermal micro-dialysis lysate – to name a few. The quantification of absorbed exogenous drugs into tissue, like vaginal biopsies and hair follicles, is also possible,” added Hiten. 

“And finally, multiple analyte analysis. In this case, the collected blood sample needs to be split into multiple aliquots for analysis, for example drug-drug interaction (DDI) studies with the Basel cocktail. The smaller sample volumes will allow more frequent sampling to be feasible and thus more accurate DDI interpretation,” Hiten explains.

“As a bio-analyst, one is seldom surprised. However, Aurora has already opened doors to new frontiers for our entire team and we cannot wait to do some more exploration,” says Hiten. 

To find out more about what Aurora and the FARMOVS team can do for your study, email business@farmovs.com

News Archive

Plant eco-physiologist finds effective solutions for crop optimisation
2016-07-24

Description: Orange trees Tags: Orange trees

The bio-stimulant was tested on
this citrus. This is the first time
that the product has been tested
on a crop.

In a time characterised by society facing increasing population growth, food crises, and extreme climatic conditions such as drought, it is essential for farmers to integrate science with their work practices in order to optimise crops.

Role of photosynthesis and plant sap data

By knowing how to use photosynthesis and plant sap data for determining plant health, fast and effective solutions could be established for the optimisation of crops. This technique, which could help farmers utilise every bit of usable land effectively, is the focus of Marguerite Westcott’s PhD study. She is a junior lecturer and plant eco-physiologist in die Department of Plant Sciences at the University of the Free State.

Westcott uses this technique in her studies to prove that a newly-developed bio-stimulant stimulates plants in order to metabolise water and other nutrients better, yielding increased crops as a result.

Agricultural and mining sectors benefit from research

The greatest part of these projects focuses on the agricultural sector. Westcott and a colleague, Dr Gert Marais, are researching the physiology of pecan and citrus trees in order to optimise the growth of these crops, thus minimising disease through biological methods. Field trials are being conducted in actively-producing orchards in the Hartswater and Patensie areas in conjunction with the South African Pecan Nut Producers Association (SAPPA) amongst others.
 
The principles that Westcott applies in her research are also used in combination with the bio-stimulant in other studies on disturbed soil, such as mine-dump material, for establishing plants in areas where they would not grow normally. This is an economical way for both the agricultural and mining sectors to improve nutrient absorption, stimulate growth, and contribute to the sustainable utilisation of the soil.

Description: Pecan nut orchards  Tags: Pecan nut orchards

The bio-stimulant contributes to the immunity of the plants.
It was tested in these pecan nut orchards (Hartswater).

Soil rehabilitation key aspect in research projects

“One of two things is happening in my research projects. Either the soil is rehabilitated to bring about the optimal growth of a plant, or the plants are used to rehabilitate the soil,” says Westcott.

Data surveys for her PhD studies began in 2015. “This will be a long-term project in which seasonal data will be collected continuously. The first set of complete field data, together with pot trial data, will be completed after the current crop harvest,” says Westcott.

 

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