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19 July 2021 | Story Lunga Luthuli | Photo Supplied
Fletcher Hiten, Chief Bioanalyst at FARMOVS, next to Aurora.

The Bioanalytical Services Division (BASD) at FARMOVS comprises a group of skilled and passionate scientists involved in the quantification of drugs, metabolites, and biomarkers in various biological matrices. One of their Analytical Science experts, Fletcher Hiten, explains what sets their team apart from the rest.

“Over the past 47 years, we have developed almost 600 validated assay methods. Most of these methods are for the analysis of ‘small’ molecules using chromatographic techniques such as LC-MS/MS, GC-MS, and HPLC, although LC-MS/MS is the technique of choice. New bioanalytical assays are continuously being development and validated in adherence to international regulatory guidelines set by the US-FDA and European Medicines Agency (EMA),” says Hiten.

“Recently, we decided to enhance our capabilities by recruiting exceptional talent. The newest member of the FARMOVS team is Aurora, a SCIEX Triple Quad™ 7500 LC-MS/MS mass analyser. Aurora is Latin for ‘dawn’: the beginning of a new era, especially one considered favourable. The SCIEX 7500 is currently marketed as the most sensitive triple quadrupole mass spectrometer available, allowing for sub-picogram/ml quantification. This means that Aurora will set FARMOVS apart from other clinical research organisations (CROs), creating an exciting and favourable landscape for clients to explore new partners in research.” 

Hiten stated: “If there was ever a time to move your next study to FARMOVS, it is now. To have Aurora on our team has many advantages, given that our clients can access unprecedented analytical sensitivity, which enables the quantification of pharmacokinetic (PK) profiles of drugs that have very low systemic absorption. These include predominantly local acting drugs, such as plasma concentrations of respiratory drugs (e.g., tiotropium and ipratropium), topically applied creams and ointments, and ophthalmology drops with ultra-sensitivity.”

“In addition, the quantification of drugs in low-volume matrices will also be exponentially enhanced, enabling the quantification of body fluids, where only a few microlitres can be collected, for example vaginal fluid, dried blood spots, cerebrospinal fluid, aqueous humour, synovial fluid, and epidermal micro-dialysis lysate – to name a few. The quantification of absorbed exogenous drugs into tissue, like vaginal biopsies and hair follicles, is also possible,” added Hiten. 

“And finally, multiple analyte analysis. In this case, the collected blood sample needs to be split into multiple aliquots for analysis, for example drug-drug interaction (DDI) studies with the Basel cocktail. The smaller sample volumes will allow more frequent sampling to be feasible and thus more accurate DDI interpretation,” Hiten explains.

“As a bio-analyst, one is seldom surprised. However, Aurora has already opened doors to new frontiers for our entire team and we cannot wait to do some more exploration,” says Hiten. 

To find out more about what Aurora and the FARMOVS team can do for your study, email business@farmovs.com

News Archive

Childhood passion becomes a successful career
2016-12-19

Description: Dr Thapelo Makae Tags: Dr Thapelo Makae 

Dr Makae took up his studies at the South Campus
of the UFS, and now serves as a community vet in Tshwane.
Photo: Anja Aucamp


Dr Thapelo Makae’s youthful passion has been a driving force in his chosen career. He says: “Like any veterinarian, my love for animals started from childhood. Growing up, I always asked myself why animals didn’t have doctors like we kids did, when our pets fell ill or died.” While veterinary services were unknown where he was raised in the Phelindaba location in Mangaung, Bloemfontein, Dr Makae started doing his own research as early as Standard 1 (Grade 3). He affirms, “I’ve always wanted to help these creatures that, it seemed, no one could help.”

Having started his academic journey on the South Campus in the CPP (as the University Preparation Programme was then known), Dr Makae obtained an undergraduate degree in Agriculture, later completing an honours degree in Agriculture. “It was at this stage,” he says, “that I was recruited by Prof Johan Greyling and the late Dr Luis Schwalbach. With their support, I completed my MSc Agric, besides having the opportunity to be a junior lecturer in Animal Physiology. Dr Schwalbach was my supervisor, my mentor, and a veterinarian himself, and I worked very closely with him. He encouraged me to pursue my passion and the dream to go ahead and study Veterinary Medicine.”

Realising that dream, Dr Makae is now employed at the Gauteng Department of Agriculture and Rural Development as a community veterinarian. Among his many responsibilities, he is charged with serving the communities of the Tshwane Metro, where he visits farmers, assisting them with health and vaccination plans, and providing advice to help them develop their skills.

Dr Makae also seeks opportunities to pass on his dream. “What I am most passionate about is going to schools and giving talks to schoolchildren, especially those from previously disadvantaged communities, who might not know much about Veterinary Medicine,” he says.

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