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18 February 2019 | Story Leonie Bolleurs | Photo HO de Waal
Ground spiny Cactus pear
Shredded, sun-dried, and coarsely ground spiny cactus pear (Opuntia ficus-indica and O. engelmannii), ready to be included in balanced diets for ruminant livestock (cattle, sheep, goats) and wild antelopes.

Prof HO de Waal, researcher in the Department of Animal, Wildlife and Grassland Sciences at the University of the Free State (UFS), has developed a standard procedure for the processing of spiny cactus pear (Opuntia spp.) into livestock fodder. This will ultimately assist in the management of massive infestations of spiny cactus pear and help to convert underutilised farmland back to natural grazing land.

In addition to applying biological control agents, mechanical control is used to harvest alien spiny cactus pear, and the large volume of material is processed as livestock feed.

 

Introduced to South Africa

 

Three hundred years ago, seafarers visiting the Cape of Good Hope introduced the well-known invading alien spiny cactus pear to South Africa. These were later transported inland and by the 1950s about one million ha of South Africa had been invaded by the alien cacti.

Some regions in the Eastern Cape have been taken over by dense, impenetrable thickets of these cacti. Invasive alien plants (IAPs) such as cacti pose a direct threat to, among others, South Africa’s water security and productive use of land.

A range of methods is used to control IAPs, including mechanical, chemical, biological, and integrated control methods.

A control programme must include the three phases of initial control to drastically reduce the existing population; follow-up control of seedlings, root suckers, and coppice growth; and maintenance control on an annual basis to sustain low alien plant numbers.

 

Processing spiny cactus pears

 

According to Prof De Waal, the harvesting and processing of the spiny cactus pear is fairly simple. Although it requires a good measure of physical strength, perseverance, and the necessary protective clothing, the cacti can be processed by harvesting the plants; shredding the cladodes through a cladode cutter; then drying them in the sun, and lastly grinding it in a hammer mill. “The long spines are degraded mechanically by grinding the sun-dried cladode strips in a hammer mill before including it in balanced livestock diets,” said Prof De Waal.

Infestations will be opened, reclaimed, rehabilitated and the natural pastures (veld) allowed to revert back to grazing for livestock.

The National Resource Management Programme (NRM)P) will be approached for official support in clearing and rehabilitating massive areas of infestation by alien spiny cactus pear in the Eastern Cape. Such financial support will be an investment in reducing the infestation by invaders and the rehabilitation and sustainable use of natural resources in South Africa.

News Archive

Newly operational sequencing unit in genomics at UFS
2016-09-09

Description: Next Generation Sequencing  Tags: Next Generation Sequencing

Dr Martin Nyaga and his research assistant,
Tshidiso Mogotsi in the Next Generation
Sequencing Laboratory.
Photo: Charl Devenish

The Next Generation Sequencing (NGS) unit at the UFS was established as an interdisciplinary facility under the Directorate for Research Development, Faculty of Health Sciences and Faculty of Natural and Agricultural Sciences.

The aim of the NGS facility is to aid internal and external investigators undertaking studies on Deoxyribonucleic acid (DNA) sequencing, assembly and bioinformatics approaches using the more advanced Illumina MiSeq NGS platform.

The NGS unit became operational in 2016 and is managed by Dr Martin Nyaga and administered through the office of the Dean, Faculty of Health Sciences, under the leadership of Prof Gert Van Zyl. Dr Nyaga has vast experience in microbial genomics, having done his PhD in Molecular Virology.

He has worked and collaborated with globally recognised centres of excellence in Prokaryotic and Eukaryotic genomics, namely the J. Craig Venter Institute and the Laboratory of Viral Metagenomics, Rega Institute, among others.

The unit has undertaken several projects and successfully generated data on bacterial, viral and human genomes. Currently, work is ongoing on bacterial and fungal metagenomics studies through 16S rRNA sequencing.

In addition, the unit is also working on plasmid/insert sequencing and whole genome sequencing of animal and human rotaviruses. The unit has capacity to undertake other kinds of panels like the HLA, Pan-cancer and Tumor 15 sequencing, among others.

Several investigators from the UFS including but not limited to Prof Felicity Burt, Prof Wijnand Swart, Dr Frans O’Neil, Dr Trudi O'Neill, Dr Charlotte Boucher, Dr Marieka Gryzenhout and Dr Kamaldeen Baba are actively in collaboration with the NGS unit.

The unit has also invested in other specialised equipment such as the M220 Focused-ultrasonicator (Covaris), 2100 Bioanalyzer system (Agilent) and the real-time PCR cycler, the Rotor-Gene Q (Qiagen), which both the UFS and external investigators can use for their research.

Investigators working on molecular and related studies are encouraged to engage with Dr Nyaga on how they would like to approach their genomics projects at the UFS NGS unit. 

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