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24 December 2018 | Story Leonie Bolleurs | Photo Anja Aucamp
Research possibilities of zebrafish exposed
Leading global genetics laboratories are replacing research on human and animal populations with zebrafish, says Prof Paul Grobler, Head of the UFS Department of Genetics.
The UFS Department of Genetics is on par with current research trends in terms of their zebrafish project. About a year has passed since they seriously started focusing on the potential of this tiny four-centimetre-long fish, and the possibilities are hugely exciting.

Looks are deceiving

Leading global genetics laboratories are replacing research on human and animal populations with zebrafish due to several fascinating reasons, of which the most profound is probably that the zebrafish share large portions of its genome with mammals. For genetics researchers this may make a lot of sense, but most people battle to see any resemblance between a six-foot-tall rugby player or 600 kg buffalo and a small, nearly transparent fish. It is in the detail, the researchers say.

Fast, effective, and visible

“The complete genome sequence of the zebrafish is known, and as much as 84% of genes known to be associated with human disease have zebrafish counterparts,” explains Head of Department, Prof Paul Grobler. Another advantage is the fast breeding rate and short generation time, and the fact that some research is ethically more justifiable when done on fish larvae rather than on adult mammals. The fact that zebrafish embryos are virtually transparent, also allow researchers to examine the development of internal structures without effort. Every blood vessel in a living zebrafish embryo is visible under a low-power microscope.

Multidisciplinary

Zebrafish provide research potential for many different study fields besides that of Prof Grobler and his team, Sue Rica Schneider and Dr Willem Coetzer. In the near future, they aim to have undergraduate students use zebrafish as a research model to develop a real sense of research and laboratory work. The Department of Chemistry are also initiating research on zebrafish housed in the Department of Genetics.

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News Archive

Sunflowers are satellite dishes for sunshine, or are they?
2016-07-20

Eighty-six percent of South Africa’s
sunflowers are produced in the
Free State and North West provinces.

Helen Mirren, the English actress, said “the sunflower is like a satellite dish for sunshine”. However, researchers at the University of the Free State (UFS) have found that too much of this sunshine could have a negative effect on the growth of sunflowers, which are a major source of oil in South Africa.

According to Dr Gert Ceronio from the Department of Soil, Crop, and Climate Sciences at the UFS, extremely high soil temperatures play a definite role in the sprouting of sunflower seedlings. Together with Lize Henning, professional officer in the department, and Dr André Nel from the Agricultural Research Council, he is doing research on biotic and abiotic factors that could have an impact on sunflowers.

Description: Sonneblom 2 Tags: Sonneblom 2

Various degrees of deformity (bad-left
to none-right) in seedlings of the same
cultivar at very high soil temperatures.
Photo: Dr Gert Ceronio

Impact of high temperatures on sunflower production

The Free State and North West provinces, which produce 86% of South Africa’s sunflowers, are afflicted especially by high summer temperatures that lead to extremely high soil temperatures.

Dr Ceronio says: “Although sunflower seeds are able to germinate at temperatures from as low as 4°C to as high as 41°C, soil temperatures of 35°C and higher could have a negative effect on the vegetative faculty of sunflower seedlings, and could have an adverse effect on the percentage of sunflowers that germinate. From the end of November until mid-January, this is a common phenomenon in the sandy soil of the Free State and North West provinces. Soil temperatures can easily exceed the critical temperature of 43°C, which can lead to poor germination and even the replanting of sunflowers.”

Since temperature have a huge impact not only on the germination of sunflower seeds, but also on the vegetative faculty and sprouting of sunflower seedlings, Dr Ceronio suggests that sunflowers should be planted in soil with soil temperatures of 22 to 30°C. Planting is usually done in October and early November. Unfortunately, this is not always possible, as soil moisture is not optimal for growth. Farmers are then compelled to plant sunflowers later.

Impact of herbicides on sunflower growth

“High soil temperatures, combined with the herbicide sensitivity of some cultivars, could lead to the poor development of seedlings," says Dr Ceronio.

The use of herbicides, such as ALACHLOR, for the control of weeds in sunflowers is common practice in sunflower production. It has already been determined that ALACHLOR could still have a damaging effect on the seedlings of some cultivars during germination and sprouting, even at recommended application dosages.

“The purpose of the continued research is to establish the sensitivity of sunflower cultivars to ALACHLOR when exposed to high soil temperatures,” says Dr Ceronio.

 

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