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13 September 2022 | Story Leonie Bolleurs | Photo Stephen Collett
Prof Trudi O’Neill, Professor in the UFS Department of Microbiology and Biochemistry, delivered her inaugural lecture on the topic: Rotavirus: New strategies to outsmart an old foe.

Prof Trudi O’Neill, Professor in the Department of Microbiology and Biochemistry at the University of the Free State (UFS) – whose research over the past 14 years is geared towards the development of a low-cost rotavirus vaccine for use in Africa – recently delivered her inaugural lecture on the Bloemfontein Campus on the topic: Rotavirus: New strategies to outsmart an old foe, a very appropriate topic for the time we live in. Most of her work is funded by the German Research Foundation.

She is a founding member and since 2016 Chairperson of the African Research Network for Neglected Tropical Diseases (ARNTD). This African-based network aims to empower current and future African researchers to support evidence-based control and elimination of neglected tropical diseases. 

Prof O’Neill, known among her colleagues as being passionate about her work, also investigates rotavirus-host interactions and strain diversity in both humans and animals. 

She obtained her PhD in Molecular Virology at the University of Pretoria in 2001 and started her career at the Onderstepoort Veterinary Institute. In 2012, she joined the Department of Microbiology and Biochemistry at the UFS.

Burden of the disease

“Rotavirus, a zoonotic infection, competes with adenovirus, cholera, and shigella as the biggest cause (27% of diarrhoea cases) of severe dehydrating diarrhoea in children under five,” says Prof O'Neill.

Repeated infections by rotavirus or other diarrhoea-causing pathogens can cause a number of long-term complications, such as intestinal damage and inflammation, nutrient loss, and malabsorption, resulting in malnutrition and subsequently a weak immune system. Malnutrition at a young age can lead to metabolic diseases such as diabetes in the long run.

She says vaccine effectiveness is dependent on many factors. Those suffering from malnutrition in poor countries with inadequate sanitation and who are living in close contact with animals, are most at risk of severe dehydrating diarrhoea caused by rotavirus infections. Low-income countries saw an efficiency rate of less than 50% compared to middle-income countries' 75% efficiency rate. She says, however, that vaccines have had the biggest public health impact in low-income countries due to the high burden of disease. 

Prof O’Neill’s presentation included a review of the research that had been conducted, including diversity studies using genome characterisation (sequencing of more than 100 strains, most of which from Mozambique), lipid studies, and investigations into the use of virus-like particles (VLPs) and subunit proteins as vaccine candidates. Production of VLPs and proteins was explored in insect cells and yeast, exploiting the Biobanks SA Yeast culture collection housed in the Department of Microbiology and Biochemistry. A subunit vaccine containing parts (proteins) of the virus that causes the disease and broad-spectrum antiviral vaccine candidates are some of the strategies she is investigating to combat the long-term effects of rotavirus infections. 

Cost and safety

Decades of work in the rotavirus field led to the licensing of the first vaccines to fight the infection in the late 2000s. Prof O’Neill says that there has been a 65% decline in rotavirus-related deaths since 2000, with vaccines being a major contributor to this. 

Two of the concerns she pointed out in terms of the vaccine were about safety and cost. For persons with severe immune deficiencies, the vaccine can cause vaccine-derived rotavirus infection.

Addressing the concern about cost, Gavi, a public–private global health partnership that aims to increase access to immunisation in poor countries, has done great work in providing rotavirus vaccine support to low-income countries. 

News Archive

Research into surrogate milk important to wildlife conservation
2017-05-08

Description: Prof Garry Osthoff  Tags: Prof Garry Osthoff

Prof Gary Osthoff from the UFS Department of
Microbial, Biochemical and Food Biotechnology,
will soon work on a milk formula for elephants.
Photo: Supplied

Research is being done at the University of the Free State (UFS) to analyse and synthetically imitate the unique milk of various wildlife species. This research is not only of scientific value, but also serves the conservation of South Africa’s wildlife species. At the forefront of this research is Prof Garry Osthoff from the Department of Microbial, Biochemical and Food Biotechnology.

Orphaned rhino calf pulled through with surrogate milk

“There is still a lot of research to be done. Naturally the research is of scientific importance, but with surrogate milk having the same composition as the mother’s milk of a specific species, orphaned calves or cubs of that species could be pulled through during a difficult time of weaning. Bearing in mind that exotic animals fetch thousands and even millions of rands at auctions, it goes without saying a game farmer will do everything possible to provide only the best nourishment to such an orphaned animal. In such a case, synthetically-manufactured milk would be the right choice,” says Prof Osthoff.

The fruits of his research were recently demonstrated in Germany when a rhino calf was left orphaned in the Leipzig Zoo. Prof Osthoff’s article: “Milk composition of a free-ranging white rhinoceros during late lactation” was used as a directive for applying surrogate milk for horse foals (which is already commercially available), since the composition of horse and rhino milk largely corresponds. The surrogate milk was used with great success and the rhino calf is flourishing. He mentions that such an orphan is often given the wrong nourishment with the best intentions, resulting in the starvation of the animal despite the amount of cow’s milk it devours.

With surrogate milk having the same
composition as the mother’s milk of a
specific species, orphaned calves or
cubs of that species could be pulled
through during the difficult time
of weaning.

Milk formula for baby elephants in the pipeline
With baby elephants left orphaned due to the increase in elephant poaching for their ivory, several attempts have been made to create a milk formula in order to feed these elephants. To date, many elephants have died in captivity from side effects such as diarrhoea as a result of the surrogate formula which they were fed.

Prof Osthoff recently received a consignment of frozen milk which he, together with researchers from Zimbabwe, will use to work on a milk formula for elephants. They are studying the milk in a full lactation period of two years. During lactation, the composition of the milk changes to such an extent that a single surrogate formula will not be sufficient. Four different formulas should probably be designed.

Prof Osthoff says that of the different species he has researched, elephants are the most interesting and deviate most from the known species.

Although his research to develop surrogate milk is adding much value to the wildlife industry, and although he finds this part of his work very exciting, his research focus is on food science and nutrition. “What is currently authentic in milk research is the study of the fat globules with content, the structure and composition of the casein micelle, and the prebiotic sugars. The knowledge which is gained helps to improve the processing, development of new food products, and development of food products for health purposes,” says Prof Osthoff.

 

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