The journal formatting for citations is reflected below:

*Corresponding author

  • 1. Jagals, P and Van Heerden, E (1998). Technical Evaluation Report on Water Quality Test Kit Components. Institute for Water Quality Studies, Department of Health, South African Bureau for Standards, Technikon Free State.
  • 2. Van Heerden, E and Litthauer, D (1999). The comparative discriminating abilities of lipase in different media and their application in fatty acid enrichment. Biocatalysis and Biotransformation. 16, 461-474. (1.085)
  • 3. Smith, DP, Kock, JLF, Van Wyk, PWJ, Venter, P, Coetzee, DJ, Van Heerden, E, Linke, D and Nigam, S (2000). The occurrence of 3-hydroxyoxylipins in the ascomycetous yeast family Lipomycetaceae. South African Journal of Science, 96, 247-249(0.726)
  • 4. Van Heerden, E, Litthauer, D, and Verger, R (2002). Biochemical characterisation and kinetic properties of the pure lipase from Aspergillus niger in bulk phase and monomolecular films. Enzyme Microbial Technology, 30(7), 902-909. (1.759)
  • 5. M Taole, J Albertyn, E van Heerden and RR Bragg (2002). Virulence of South African Isolates of Haemophillus Paragallinarium. Part 3: Experimentally produced NAD independent isolate. Onderstepoort Journal of Veterinary Research, 69, 189-196. (0.514)
  • 6. Smith, DP, Kock, JLF, Van Wyk, PWJ, Pohl, CH, Van Heerden, E, Botes, PJ, Nigam, S (2003) Oxylipins and ascospore morphology in the ascomycetous yeast genus Dipodascus. Antonie. v. Leeuwenhoek. 83(4), 317-325. (2.915)
  • 7. O de Smidt, J Albertyn, RR Bragg and E van Heerden (2004). Genetic organisation of the capsule transport gene region from Haemophilus paragallinarum. Onderstepoort Journal of Veterinary Research. 71, 139-152. (0.514)
  • 8. RR Bragg, P Jansen van Rensburg, E van Heerden, and J Albertyn (2004). The testing and modification of a commercially available transport medium for the transportation of pure cultures of Haemophilus paragallinarum for serotyping. Onderstepoort Journal of Veterinary Research. 71, 93- 98. (0.514)
  • 9. Van Heerden, E* and Litthauer, D (2004). Optimisation of reaction conditions for selective ester formation by lipase using a factorial design. South African Journal of Science. 100(5). 249-250. (0.726)
  • 10. Joseph, M, Kock, JLF, Pohl, CH, Botes, PJ, Van Heerden, E and Hugo, A (2005). Acetate-enhanced polymerised triacylglycerol utilisation by Mucor circinelloides. World Journal of Microbiology and Biotechnology. 21, 97-99. (0.514)
  • 11. Pfiffner S, Cantu J, Smithgall A, Peacock A, White D, Moser D, Onstott T, Van Heerden E (2006). Deep subsurface microbial biomass and community structure in Witwatersrand basin mines. Geomicrobiology Journal 23(6), 369-414 (0.759).
  • 12. Onstott, TC; Lin, LH; Davidson, M; Mislowack, B; Borcsik, M; Hall, J; Slater, C; Ward, J; Lollar, B Sherwood; Lippmann-Pipke, J; Boice, E; Pratt, LM; Pfiffner, S; Moser, D; Gihring, T; Kieft, Thomas L; Phelps, Tommy J; Van Heerden, E; Litthauer, D; Deflaun, M (2006). The Origin and Age of Biogeochemical Trends in Deep Fracture Water of the Witwatersrand Basin, South Africa. Geomicrobiology Journal. 23(6), 369-414.
  • 13. Möller, C and Van Heerden, E* (2006). The isolation of a soluble and membrane-associated Fe(III) reductase from the thermophile, Thermus scotoductus (SA 01). FEMS 265(2), 237-243. (1.840)
  • 14. DeFlaun, MF, JK Fredrickson, H Dong, SM Pfiffner, TC Onstott, DL Balkwill, S Streger, E Stackebrandt, Knoesen, S and E van Heerden. (2007) Isolation and characterisation of a novel Geobacillus thermoleovorans species from an ultra-deep South African gold mine. Systematic and Applied Microbiology. 30, 152-164. (2.417)
  • 15. Opperman, DJ and Van Heerden E* (2007). Aerobic Cr(VI) reduction by Thermus scotoductus strain SA-01. Journal of Applied Microbiology, 103, 1907-1913. (2.006)
  • 16. Botes E, Van Heerden E*, and Litthauer D (2007). Hyper-resistance to arsenic in bacteria isolated from an antimony mine in South Africa. S. Afr. J. Sci. 103, 279-281. (0.726)
  • 17. TG Barnard, E van Heerden*, RR Bragg and J Albertyn. (2007). Haemophillus paragallinarum's haemagglutinin - characterisation of structure and its possible role in adhesion and serotyping. Onderstepoort Journal of Veterinary Research vol. 75, March 2008, pp 11-16. (0.514)
  • 18. CH Pohl, W Kriel, P Venter, E van Heerden and J Albertyn. (2007) The diversity of culturable airborne fungi in an active South African gold mine. S. Afr. J. Sci. 103, 277-278 (0.726)
  • 19. Opperman, DJ and Van Heerden, E* (2008): A membrane-associated protein with Cr(VI) reducing activity from Thermus scotoductus SA-01. FEMS Microbiology Letters 280: 210-218 (2.723)
  • 20. Opperman, DJ, Piater, LA, and Van Heerden, E* (2008). A novel chromate reductase from Thermus scotoductus SA-01 related to Old Yellow Enzyme Journal of Bacteriology, p. 3076–3082 (2.688)
  • 21. Ojo, AO, Van Heerden, E, and Piater, LA (2008). Identification and initial characterisation of a copper resistant South African mine isolate. African Journal of Microbial Research, 2, 281-287
  • 22. Van Heerden, E*, Opperman, DJ, Bester, AP, Van Marwijk, J, Cason, ED, Litthauer, D, Piater, LA, and Onstott, TC (2008). Metabolic promiscuity from the deep subsurface: a story of Survival or Superiority. Instruments, Methods, and Missions for Astrobiology XI. Edited by Hoover, Richard B; Levin, Gilbert V; Rozanov, Alexei Y; Davies, Paul C. Proceedings of the SPIE, Volume 7097, pp. 70970S-70970S-12.
  • 23. Raji, AI; Möller, C; Litthauer, D; Van Heerden, E, and Piater, LA (2008). Bacterial diversity of biofilm samples from deep mines in South Africa. Biokemistri, Vol. 20, No. 2, pp. 53-62.
  • 24. Rashamuse, K; Ronnenburg, T; Hennesssy, F; Visser, D; Van Heerden, E; Piater, LA; Litthauer, D; Möller, C; and Brady, D (2009). Discovery of a novel carboxylesterase through functional screening of a pre-enriched environmental library. Journal of Applied Microbiology. 16 Feb 2009. Vol106(5) 1532-1539.
  • 25. Van Marwijk, J, Opperman, DJ, Piater, LA, and Van Heerden, E* (2009). Reduction of vanadium(V) by Enterobacter cloacae EV-SA01 isolated from a South African deep gold mine. Biotechnology Letters: Volume 31, 6,p 845-849
  • 26. Jordan, R, Van Heerden, E, Hugo, CJ, and Piater, LA (2009). Using current molecular techniques for rapid differentiation of Salmonella Typhi and Salmonella Typhimurium. Accepted in African Journal of Biotechnology. African Journal of Biotechnology Vol. 8 (9), pp. 1815–1818, 4 May 2009.
  • 27. Bester, PA, Litthauer, D, Piater, LA, and Van Heerden, E* (2010). A thioredoxin reductase-like protein from the thermophile, Thermus scotoductus SA-01,displaying iron reductase activity. FEMS Microbiol Lett 302, 182-188.
  • 28. Opperman, DJ, Sewell, BT, Litthauer, D, Isupov, MN, Littlechild, JA, and Van Heerden, E* (2010). Crystal Structure of a Thermostable Old Yellow Enzyme from Thermus scotoductus SA-01. BBRC (Biochemical and Biophysical Research Communications) Volume 393, Issue 3, p 426- 431.
  • 29. Litthauer, D, Abbai, NS, Piater, LA, and Van Heerden, E (2010). Pitfalls using tributyrin agar screening to detect lipolytic activity in Metagenomics studies. African Journal of Biotechnology, Vol 9(27), pp4282-4285. July 2010.
  • 30. Onstott, TC, Lurdoch, L, and Van Heerden, E (2010). Deep subsurface microbiology after 25 years. BRGM Geosciences July. 2010 – 52–59.
  • 31. E Botes, R Jordan, MF DeFlaun, J Howell, R Borch, FC Liebenberg, E van Heerden. Bioremediation of Hexavalent Chromium Contaminated Water In Fixed-Film Upflow Reactors – a South African First. Journal of Biotechnology, vol 150 pp 269-269, 2010.
  • 32. E Botes, R Jordan, MF DeFlaun, J Howell, R Borch, PD Price, E van Heerden. Bioremediation Using a Two-Phase Bio / Abiotic Approach To Treat Acid Mine Drainage in South Africa. Journal of Biotechnology, vol 150, pp269-270, 2010.
  • 33. Johanna Lippmann-Pipke, Barbara Sherwood Lollar, Samuel Niedermann, Nicole A Stroncik, Rudolf Naumann; Esta van Heerden, Tullis C Onstott (2011). Neon identifies two billion year old fluid component in Kaapvaal Craton. Chemical Geology – 283 (2011) 287–296.
  • 34. Borgonie, G, García-Moyano, A, Litthauer, D, Bert, W, Bester, PA, Van Heerden, E, Möller, C, Erasmus, M and Onstott, TC (2011). Worms from hell: Nematoda from the terrestrial deep subsurface of South Africa. Nature 2009-12-15946E, volume 474, issue 7349 from 2 June 2011.
  • 35. Johanna Lippmann-Pipke, Jörg Erzinger, Martin Zimmer, Christian Kujawa, Margaret Boettcher, Esta van Heerden, Armand Bester, Hannes Moller, Nicole A Stroncik, Zeev Reches. Direct observation of geogas transport through an inactive fault system at 3.54 km depth, TauTona gold mine, South Africa. Applied Geochemistry –. Volume 26, Issue12, December 2011, pages 2134-2146.
  • 36. Bernard Slippers, Thokozani Majozi, Fulufhelo V Nelwanondo, Christine M Steenkamp, Esta van Heerden and Caradee Y Wright. Internet access is a constraint for science development and training at South African Universities –South African Journal of Science, 107 (9-10) (2011).
  • 37. Abbai, NS, Van Heerden, E, Piater, LA, Litthauer, D (2011). A deep gold mine metagenome as a source of novel Esterases. African Journal of Biotechnology 10 (32), pp. 6090-6100
  • 38. Kamini Gounder, Elzbieta Brzuszkiewicz, Heiko Liesegang, Antje Wolherr, Rolf Daniel, Gerhard Gottschalk, Oleg Reva, Benjamin Kumwenda, Malay Srivastava, Carlos Bricio, José Berenguer, Esta van Heerden, and Derek Litthauer. (2011). Sequence of the hyperplastic genome of the naturally competent Thermus scotoductus SA-01 – BMC Genomics; Vol. 12, No. 1,
  • 39. Sherwood Lollar, B, Van Heerden, E, Onstott, TC, Kieft, T. (2011) Carbon cycling in the deep crustal biosphere Elements 7 (2), pp. 137.
  • 40. Julie Bernard, Esta van Heerden, Isabel WCE Arends, Dirk J Opperman, and Frank Hollmann (2011). Chemoenzymatic reduction of conjugated C=C-double bonds. Chem Cat Chem. Volume 4, Issue 2, pages 196-199.
  • 41. César, CE, Álvarez, L, Bricio, C, Van Heerden, E, Littauer, D, Berenguer, J (2011). Unconventional lateral gene transfer in extreme thermophilic bacteria 2011International Microbiology 14 (4) , pp. 187-199.
  • 42. Errol Duncan Cason, Lizelle Anne Piater, and Esta van Heerden. Reduction of U(VI) by the deep subsurface bacterium, Thermus scotoductus SA-01, and the novel involvement of the ABC transporter protein –Vol86, Issue 6, pages 572-577 Chemosphere (2012).
  • 43. Christoph K Winkler, Dorina Clay, Esta van Heerden, Kurt Faber. (2013). Overcoming Co-Product Inhibition in the Nicotinamide Independent Asymmetric Bioreduction of Activated C=C-Bonds Using Flavin-Dependent Ene-Reductases. Biotechnology and Bioengineering. Impact 3.95 – Dec 2013 110(2): 3085-92
  • 44. Onstott, TC, Magnabosco, C, Aubrey, AD, Burton, AS, Dworkin, JP, Elsila, JE, Grunsfeld, S, Cao, BH, Hein, JE, Glavin, DP, Kieft, TL, Silver, BJ, Phelps, TJ, Van Heerden, E, Opperman, DJ, and Bada, JL. 2014. Does aspartic acid racemization constrain the depth limit of the subsurface biosphere? Geobiology 12: 1-19. Hybrid code 31, CESM code 13, Impact factor 3.692
  • 45. Litthauer, S, Gargiulo, S, Van Heerden, E, Hollmann, F, Opperman, DJ. Heterologous expression and characterization of the ene-reductases from Deinococcus radiodurans and Ralstonia metallidurans. Journal of Molecular Catalysis B: Enzymatic 99, pp. 89-95 (2014)
  • 46. Peter J Williams, Elsabe Botes, Maleke M Maleke, Abidemi Ojo, Mary F DeFlaun, Jim Howell, Robert Borch, Robert Jordan, Esta van Heerden. Effective bioreduction of hexavalent chromium-contaminated water in fixed-film bioreactors. 2014. Water SA 40 (3): 549-554.
  • 47. Lau, MCY, Cameron, C, Magnabosco, C, Brown, CT, Schilkey, F, Grim, S, Hendrickson, S, Pullin, M, Sherwood-Lollar, B, Van Heerden, E, Kieft, TL, and Onstott, TC. 2014. Phylogeny and phylogeography of functional genes shared among seven terrestrial subsurface metagenomes reveal N-cycling and microbial evolutionary relationships. Frontiers in Microbiology 5:531. Impact factor 3.9
  • 48. Magnabosco, C, Tekere, M, Lau, MCY, Linage, B, Kuloyo, O, Erasmus, M, Cason, E, Van Heerden, E, Borgonie, G, Kieft, TL, Olivier, J, and Onstott, TC. 2014. Comparisons of the composition and biogeographic distribution of the bacterial communities occupying South African thermal springs with those inhabiting deep subsurface fracture water. Frontiers in Microbiology 5:679. Pp1-17. Impact factor 3.9 doi:  10.3389/fmicb.2014.00679
  • 49. Blanco, Y, Rivas, LA, García-Moyano, A, Aguirre, J, Cruz-Gil, P, Palacín, A, Van Heerden, E, and Parro, V. 2014. Deciphering the prokaryotic community and metabolisms in South African deep-mine biofilms through antibody microarrays and graph theory. PLoS ONE DOI:1371e114180. 1-26 , Impact factor 3.53
  • 50. Maleke Maleke, Peter Williams, Julio Castillo, Elsabe Botes, Abidemi Ojo, Mary DeFlaun and Esta van Heerden. 2014. Optimization of a bioremediation system of soluble uranium based on the biostimulation of an indigenous bacterial community. Environmental Science and Pollution Research. Impact factor 2.87
  • 51. Erasmus, M, Cason, ED, Van Marwijk, J, Botes, E, Gericke, M, Van Heerden, E. 2015. Gold nanoparticle synthesis using the thermophilic bacterium Thermus scotoductus SA-01 and the purification and characterization of its unusual gold reducing protein. Gold Bulletin 47(4):245-253, Impact factor 1.84.
  • 52. Galina B Slobodkina, PhD; Olga L Kovaleva; Margarita L Miroshnichenko; Alexander I Slobodkin; Tatyana V Kolganova; Andrei A Novikov; Esta van Heerden; Elizaveta A BonchOsmolovskaya 2015. Thermogutta terrifonta gen. nov., sp. nov. and Thermogutta hypogea sp. nov., novel thermophilic anaerobic representatives of the phylum Planctomycetes. International Journal of Systematic and Evolutionary Microbiology – Volume 65, issue 3, 2015 Pages 760-765. Impact factor 2.7.
  • 53. Julio Castillo, Alba Gomez-Arias, Jan Posthumus, Megan Welman-Purchase, and Esta van Heerden. Geochemical Study of the Interaction of Acid and Alkaline Mine Drainage with BaCO3. Paper no 290, Pages 1-12. Proceedings 2015 of the 10th ICARD | IMWA 2015 Conference – Agreeing on solutions for more sustainable mine water management. Santiago, Chile. ISBN 978-956-9393-27.
  • 54. Alba Gomez-Arias, Julio Castillo, Megan Welman-Purchase, Jan Posthumus, and Esta van Heerden. Evidences of Effective Treatment of Alkaline Mine Drainage with BaCO3 Paper no 303, Pages 1-12. Proceedings 2015 of the 10th ICARD | IMWA 2015 Conference – Agreeing on solutions for more sustainable mine water management. Santiago, Chile. ISBN 978-956-9393-27.
  • 55. Alba Lozano, Carlos Ayora, Francisco Macias, Jose Miguel Nieto, Alba Gomez-Arias, Julio Castillo and Esta van Heerden. Sulphate removal from Acid Mine Drainage: evaluation of BaCO3 with column experiments. Macla 19 July 2015 (p. 82-83).
  • 56. Jessica Labonté, Erin Field, Maggie Lau, Dylan Chivian, Esta van Heerden, KE Wommack, Thomas L Kieft, Tullis Onstott, Ramunas Stepanauskas (2015). Single cell genomics indicates horizontal gene transfer and viral infections in a deep subsurface Firmicutes population. Frontiers in Microbiology. Volume 6 Issue APR, 2015 Article number 349. pp. 1-11, doi: 10.3389/fmicb.2015.00349.
  • 57. Danielle N Simkus, Greg F Slater, Barbara Sherwood Lollar, Kenna Wilkie, Thomas L Kieft, Cara Magnabosco, Maggie CY Lau, Michael J. Pullin, Sarah B Hendrickson, K Eric Wommack, Eric G Sakowski, Esta van Heerden, Olukayode Kuloyo, Borja Linage, Gaetan Borgonie, Tullis C Onstott. (2015) Variations in microbial carbon sources and cycling in the deep continental subsurface. Geochimica et Cosmochimica Acta GCA-D-14-00916R3
  • 58. G Borgonie, B Linage-Alvarez, A Ojo, S Shivambu, O Kuloyo, ED Cason, S Maphanga, J Vermeulen, D Litthauer, CD Ralston, TC Onstott, B Sherwood-Lollar, and E van Heerden (2015). Deep subsurface mine stalactites trap endemic fissure fluid Archaea, Bacteria and Nematoda possibly originating from ancient (inland) seas. Frontiers of Microbiology, 2015 Volume 6 Issue JUL, Article number 833.
  • 59. G Borgonie, B Linage-Alvarez, AO Ojo, SOC Mundle, LB. Freese, C van Rooyen, O Kuloyo, J Albertyn, C Pohl, ED Cason, J Vermeulen, C Pienaar, D Litthauer, H van Niekerk, J van Eeden, B Sherwood Lollar, TC Onstott, and E van Heerden (2015). Eukaryotic opportunists rule the deep subsurface biosphere in South Africa. Nature Communications. Nov 2015. DOI: 10.1038/ncomms9952
  • 60. Cara Magnabosco*, Kathleen Ryan, Maggie CY Lau, Olukayode Kuloyo, Barbara Sherwood Lollar, Thomas L Kieft, Esta van Heerden, TC Onstott. A Metagenomic Window into Prokaryotic Carbon Metabolism at 3 km Depth in Precambrian Continental Crust. ISME J, 2015 Sept. Epub doi: 10.1038/ismej.2015.150. Impact Factor 9.302 (Multidisciplinary Journal of Microbial Ecology). Volume 10, Issue 3, Pages 730-741 published 1 March 2016.
  • 61. Magnabosca, C, Timmers, PHA, Lau, MCY, Borgonie, G, Linage-Alvarez, B, Kuloyo, O, Alleva R, Kieft, TL, Slater, GS, Van Heerden E, Sherwood Lollar, B, and Onstott, TC. The case for a dynamical subsurface ecosystem. BioRxiv doi:10.1101/040204.
  • 62. WJ Müller, N Tlalajoe, ED Cason, D Litthauer, O Reva, E Brzuszkiewicz, and E van Heerden (2016). Whole Genome Comparison of Thermus sp. NMX2 A1 Reveals Principle Carbon Metabolism Differences with Closest Relation Thermus scotoductus SA-01. G3-Genes Genomes Genetics 6(9) · July 2016. DOI: 10.1534/g3.116.032953.
  • 63. Podosokorskaya OA, Merkel AY, Gavrilov SN, Fedoseev I, Heerden EV, Cason ED, Novikov AA, Kolganova TV, Korzhenkov AA, Bonch-Osmolovskaya EA, Kublanov IV. (2016) Tepidibacillus infernus sp. Nov., a moderately thermophilic, selenate-and arsenate-respiring hydrolytic bacterium isolated from a gold mine, and emended description of the genus Tepidibacillus. Int J Syst Evol Microbiol. Volume 66, Issue 8, August 2016, Article number 001166, Pages 3189-3194. doi:0.1099/ijsem.0.001166.
  • 64. Alba Gomez, Julio Castillo, Esta van Heerden, Danie Vermeulen. Use of alkaline mine waste as treatment for acid drainage. 2016. IMWA-Freiburg, Germany 2016 Proceedings (International Mine Water Association). 11th IMWA Annual Conference – Mining Meets Water – Conflicts and Solutions. Paper 223. p. 931–936.
  • 65. Maggie CY Lau, Thomas L Kieft, Olukayode Kuloyo, Borja Linage-Alvarez, Esta van Heerden, Melody R Lindsay, Cara Magnabosco, Wei Wang, Jessica B Wiggins, Ling Guo, David H Perlman, Saw Kyin, Henry H. Shwe, Rachel L Harris, Youmi Oh, Min Joo Yi, Roland Purtschert, Greg F Slater, Shuhei Ono, Siwen Wei, Long Li, Barbara Sherwood Lollar, and Tullis C Onstott. An oligotrophic deep-subsurface community dependent on syntrophy is dominated by sulfur-driven autotrophic denitrifiers. PNAS vol 113 no. 49 E7927–E7936, doi: 10.1073/pnas.161224411
  • 66. MO Agunbiade, Esta van Heerden, CH Pohl and AOT. Ashafa. Flocculating performance of a bioflocculant produced by Arthrobacter humicola in sewage waste water treatment. BMC Biotechnol. 2017 Jun 12;17(1):51. doi: 10.1186/s12896-017-0375-0.
  • 67. Cason ED, Williams PJ, Ojo E, Castillo J, DeFlaun MF, Van Heerden E. Hexavalent chromium bioreduction and chemical precipitation of sulphate as a treatment of site-specific fly ash leachates. World J Microbiol Biotechnol. 2017 May;33(5):88. doi: 10.1007/s11274-017-2243-4. Epub 2017 Apr 7.


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