Mrs Laurinda Steyn
Position
Lecturer
Department
Microbiology and Biochemistry
Telephone
0514017468
Office
A55
Short CV

University of the Free State

Department of Microbiology and  Biochemistry

South Africa

steynl@ufs.ac.za

Phone: +27 51 401 7468

Mobile: +27 84 608 5698

Website: http://www.ufs.ac.za/

Curriculum Vitae of Laurinda Steyn (6604090020085)

Education

 


Jan 2002 – Dec 2006

University of the Free State

Master of Science, MSc Biotechnology (part-time)

Bloemfontein, South Africa

Jan 1984 – Dec 1989

Central University of Technology

Bachelor of Technology, Medical Technician, Chemical Pathology (Part-time)

Bloemfontein, South Africa

Teaching Experience

Modules

MCBG3614 Microbial Growth, Nutrition and Death (Practical) 2011-2015

MCBT6814 Microbial Techniques (Growth, Safety, Centrifugation, Data Modeling): 2013 - Current

MCBH2614 Introduction to Microbiology for Health and Consumer Science. 2017 - Current

MCBH2624 Pathogenic Microorganism for Health Science, 2016 - Current

Languages

Afrikaans, English

Work Experience

Jan 2018 - Current

Lecturer / Researcher

The University of the Free State, Department of Microbial, Biochemical and Food Biotechnology.

Fermentation biotechnology with a special interest in continuous cultures, yeast physiology and the production of metabolites such as bioethanol, Growth kinetics and physiology

Chair Safety Committee of Dept. Microbiology and Biochemistry. Qualified Fire and Evacuation Officer and Safety Representative

UFS Biosafety and Environmental Ethics Committee

Jan 2007 – Dec 2017

 

 

 

 

Researcher

The University of the Free State, Department of Microbial, Biochemical and Food Biotechnology

Bloemfontein, South Africa

Chair Safety Committee of Dept. Microbial, Biochemical and Food Biotechnology. Qualified Fire and Evacuation Officer and Safety Representative

Apr 2000 – Dec 2006

Assistant Researcher

The University of the Free State, Department of Microbial, Biochemical and Food Biotechnology

Bloemfontein, South Africa

Jul 1999 – Mar 2000

Development Fermentation Laboratory Manager

AECI Bioproducts (now SA Bioproducts), Research and Development

Durban, KwaZulu-Natal, South Africa

Development of Lysine production with C. glutamicum

Jan 1998 – Jun 1999

Development Fermentation Program Coordinator

AECI Bioproducts, Research and Development

Durban, KwaZulu-Natal, South Africa

Development of Lysine production with C. glutamicum

Nov 1994 – Dec 1997

Development Biotechnologist

AECI Bioproducts, Research and Development

Durban, KwaZulu-Natal, South Africa

Development of Lysine production with C. glutamicum

Apr 1990 – Sep 1994

Laboratory technician

The University of the Free State, Department of Microbial, Biochemical and Food Biotechnology

Bloemfontein, South Africa

Dec 1983 – Mar 1990

Medical Technician

Drs Voigt and Partners, Chemical Pathology

Bloemfontein, Orange Free State, South Africa

Awards & Grants

Jan 2014

Grant: NRF Thuthuka PhD track

Jan 2013

Grant: NRF Thuthuka PhD track

Jan 2012

Grant: NRF Thuthuka PhD track

Skills & Activities

Skills

Yeasts, Fermentation, Ethanol Production, Industrial Biotechnology, Anaerobic Culture, Microbial Biotechnology, Bioprocess Engineering and Fermentation Technology, Microbiological Processes, Fermentation Biotechnology, Fermentation Technology, Microbial Fermentation, Industrial Microbiology, Bioprocess Engineering, Yeast Fermentation, Biotechnology Industry, Ethanol Fermentation, Biotechnological Engineering, Yeast Biotechnology, Microbial Physiology, Biotransformation, Fermentation Techniques, Environmental Biotechnology, SSF, Saccharomyces cerevisiae, Alcohol Dehydrogenase, Ethanol, Biomass, Bioprocess Technology, Applied Microbiology, Bioprocess optimization, Vaccine production in Yeast, Beer brewing, Microbial pathology, Health science microbiology.

Fire and Evacuation Training

Safety Representative Training

Fundamentals of Animal Research Ethics Training

Languages

Afrikaans, English

Scientific Memberships

South Africa Society for Microbiologists

Departmental Support

University Support

Health and Safety Committee Chair

Biosafety and Environmental Ethics Committee


Publications

 Researcher ID

Journal Publications

Oosthuizen L, Charimba G, Hitzeroth A, Nde AL, Steyn L, Newman J, Hugo C (2019) Chryseobacterium pennipullorum sp. nov., isolated from poultry feather waste. Int J Syst Evol Microbiol DOI: 10.1099/ijsem.0.003491. Impact Factor: 2.166

Jiru TM, Steyn L, Pohl C, Abate D (2018) Production of single cell oil from cane molasses by Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89 as a biodiesel feedstock. Chem Cent J 12(1)91. DOI: 10.1186/s13065-018-0457-7. Impact factor 1.22

Jiru TM, Groenewald M, Pohl C, Steyn L, Kiggundu N, Abate D (2017): Optimization of cultivation conditions for biotechnological production of lipid by Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89 for biodiesel preparation. 3 Biotech; 2/145. DOI: 10.1007/s13205-017-0769-7. Impact Factor: 1.361

Schabort DWP, Letebele PK, Steyn L, Kilian SG, du Preez JC (2016) Differential RNA-seq, Multi-Network Analysis and Metabolic Regulation Analysis of Kluyveromyces marxianus reveals a Compartmentalised Response to Xylose. PLoS ONE, 11(6). DOI:10.1371/journal.pone.0156242 Impact Factor: 2.806

Bekker A, Steyn L, Charimba G, Jooste P, Hugo C (2015) Comparison of the growth kinetics and proteolytic activities of Chryseobacterium species and Pseudomonas fluorescens. Can J Microbiol DOI: 10.1139/cjm-2015-0236 Impact Factor: 1.335

Bekker A, Jooste P, Steyn L, Bothma C, Hugo A, Hugo C (2015) Lipid breakdown and sensory analysis of milk inoculated with Chryseobacterium joostei or Pseudomonas fluorescens. Int Dairy J; 52. DOI:10.1016/j.idairyj.2015.09.003. Impact Factor: 2.067

Akanni GB, du Preez JC, Steyn L, Kilian SG (2014): Protein enrichment of an Opuntia ficus-indica cladode hydrolysate by cultivation of Candida utilis and Kluyveromyces marxianus. J Sci Food Agric; 95(5). DOI:10.1002/jsfa.6985. Impact Factor: 1.714

Kuloyo OO, du Preez JC, Del Prado García-Aparicio M, Kilian SG, Steyn L, Görgens J (2014) Opuntia ficus-indica cladodes as feedstock for ethanol production by Kluyveromyces marxianus and Saccharomyces cerevisiae. World J Microbiol Biotech; 30(12). DOI: 10.1007/s11274-014-1745-6. Impact Factor: 1.658

OO Ezekiel, OC Aworth, JC du Preez, L Steyn: Cultivation of Candida utilis on cassava peel hydrolysates for single-cell protein production. Journal of Food Science and Engineering 2 / 2012; 452-461

  

Conference Proceedings

Cason ED, Valverde A, Viljoen B, Vermeulen J, Vermeulen M, Steyn L, de Smidt O, Malefane M (2019) Unravelling the microbiome of Sesotho (traditional Lesotho sorghum beer) through targeted metagenomics. FEMS 2019, Glasgow, Scotland. 7-11 July 2019 PW404.

Nde AL, Charimba G, Hitzeroth A, Oosthuizen L, Steyn L, Newman J, Hugo C (2019) Whole-genome sequencing of a novel Chryseobacterium strain isolated from poultry feather waste. FEMS 2019, Glasgow, Scotland. 7-11 July 2019 PT403.

Oosthuizen L, Charimba G, Hitzeroth A, Lum Nde A, Steyn L, Newman J, Hugo C (2019) Chryseobacterium pennipullorum sp. nov., isolated from poultry feather waste. FEMS 2019, Glasgow, Scotland. 7-11 July 2019. PT404.

Dithebe K, van Wyk PWJ, Steyn L, Pohl CH (2018) Characterization of intracellular gas bubbles in Saccharomyces. SASM 2018. Muldersdrift, South Africa. 4-7 April 2018.

Steyn L, du Preez JC, Albertyn J, de Smidt O (2014) Phenotypic analysis of multiple ADH deletion mutants of Saccharomyces cerevisiae. ISSY 2014, Nova Gorica & Vipava, Slovenia. 9 - 12 October. Presentation.

Steyn L, du Preez JC, Albertyn J, de Smidt O (2013) Phenotype microarray analysis of multiple ADH deletion mutants of Saccharomyces cerevisiae. FEMS 2013, Leipzig, Germany. 21-25 July 2013. Poster.

du Preez JC, Steyn L, de Smidt O, Albertyn J (2012) The physiological role of alcohol dehydrogenase 5 during chemostat cultivation of Saccharomyces cerevisiae ICY 2012, Madison, Wisconsin, USA September.

Bekker A, Hugo C, Jooste P, Steyn L (2011) Effect of temperature on growth and proteolytic activity of Chryseobacterium in milk. SASM 2011, Cape Town, South Africa.

Alkani G, du Preez JC, Steyn L, Kilian SG (2011) Evaluation of yeast single-cell protein production using a hydrolysate of Opuntia ficus-indica biomass as carbon feedstock. SASM 2011, Cape Town, South Africa.

Meyer A, du Preez JC, Steyn L, Kilian SG (2011) The critical dissolved oxygen level in respect of glucose and xylose utilisation by Kluyveromyces marxianus. SASM 2011, Cape Town, South Africa.

Steyn L, de Smidt O, du Preez JC, Albertyn J, Kilian SG (2011) The physiological role of alcohol dehydrogenase 5 (ADH5p) isozyme in Saccharomyces cerevisiae. SASM 2011, Cape Town, South Africa.

Kuloyo O, Steyn L, du Preez JC (2011) A comparative evaluation of Kluyveromyces marxianus and Saccharomyces cerevisiae for biotehanol production from Opuntia ficus. ISSY29, Guadalajara, Mexico. September.

Albertyn J, de Smidt O, Dukunde A, Henn, Steyn L, du Preez JC (2009) Molecular and physiological aspects of alcohol dehydrogenase in the yeast Saccharomyces cerevisiae. SASM 2009, Durban, South Africa.

du Preez JC, Steyn L, de Smidt O, Dukunde A, Albertyn J (2009) Phenotypic characteristics of multiple ADH deletion mutnats of Saccharomyces cerevisiae. ISSY27, Paris, France. October 2009.

Steyn L, de Smidt O, Ntlhokoa K, Albertyn J, du Preez JC (2008) The role of alcohol dehydrogenase 1 and 2 in Saccharomyces cerevisiae. SASM 2008.

Research

Yeasts, Fermentation, Ethanol Production, Industrial Biotechnology, Anaerobic Culture, Microbial Biotechnology, Bioprocess Engineering and Fermentation Technology, Microbiological Processes, Fermentation Biotechnology, Fermentation Technology, Microbial Fermentation, Industrial Microbiology, Bioprocess Engineering, Yeast Fermentation, Biotechnology Industry, Ethanol Fermentation, Biotechnological Engineering, Yeast Biotechnology, Microbial Physiology, Biotransformation, Fermentation 

Area(s) of Interest

Fermentation biotechnology with a special interest in growth kinetics in batch, fed-batch and continuous cultures; yeast physiology and the production of metabolites such as bioethanol.

Fundamental Microbiology with special application in Health Sciences.

Expertise
Yeasts, Fermentation, Ethanol Production, Industrial Biotechnology, Anaerobic Culture, Microbial Biotechnology, Bioprocess Engineering and Fermentation Technology, Microbiological Processes, Fermentation Biotechnology, Fermentation Technology, Microbial Fermentation, Industrial Microbiology, Bioprocess Engineering, Yeast Fermentation, Biotechnology Industry, Ethanol Fermentation, Biotechnological Engineering, Yeast Biotechnology, Microbial Physiology, Biotransformation, Fermentation 
Courses Presented

MCBG3614 Microbial Growth, Nutrition and Death (Practical) 2011-2015

MCBT6814 Microbial Techniques (Growth, Safety, Centrifugation and Data Modeling): 2013 - Current

MCBH2614 Introduction to Microbiology for Health and Consumer Science. 2017 - Current

MCBH2624 Pathogenic Microorganism for Health Science, 2016 - Current

 

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BLOEMFONTEIN CAMPUS FACULTY CONTACT

Elfrieda van den Berg (Marketing Manager)
T: +27 51 401 2531
E:vdberge@ufs.ac.za

QWAQWA CAMPUS FACULTY CONTACT

Dilahlwane Mohono (Faculty Officer)
T: +27 58 718 5284
E:naturalscienceqq@ufs.ac.za

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