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17 April 2019 | Story Leonie Bolleurs
Science ambassadors
Friends Tekano Mbonani and Chaka Mofokeng are pursuing graduate degrees in respectively Physics at the University of the Free State (UFS) and Astronomy at the University of the Western Cape. The two got together and decided to reach out to the high school, Leseding Technical Secondary School, where they came from.

It was a full house as more than 120 learners packed the hall at the Leseding Technical Secondary School in the Free State, where two young Astronomy researchers had come home to tell their younger peers about their studies and career prospects across South Africa.

Chaka Mofokeng and Tekano Mbonani are both former learners at the high school. Currently pursuing graduate degrees – for Mbonani in Physics at the University of the Free State (UFS), and for Mofokeng in Astronomy at the University of the Western Cape – the two friends got together and decided to reach out to the high school where they came from.

The event took place in January before schoolwork, tests, and exam preparations are occupying learners’ minds, inviting them to think about the big picture – the future, and how to be part of it. This is timely, because in July last year, the MeerKAT radio telescope was inaugurated in the Karoo. The MeerKAT is the first step to the international SKA telescope project, but it is already one of the best radio telescopes in the world and has placed South Africa firmly on the world map of radio astronomy and engineering.

Building a bridge
“This project enables us to build a bridge between secondary and tertiary institutions. Currently focused on senior secondary students, we aim to promote science through outreach events and activities. Using science and technology-based activities and events, such as stargazing at an observatory or exploring the universe in a planetarium, we want to attract these future secondary graduates. We also provide mentorship, hoping to help them improve their academic performance in matric,” said Mbonani.

For a whole morning, they spoke about their journeys, about science, about the skills that scientists acquire during their studies and all the opportunities such studies open up in an era where the 4th Industrial Revolution is predicted to reduce the number of jobs in many traditional professions. They addressed their peers in both English and Sesotho.

Astronomy in South Africa contributes to critical-skills development. Investing in the MeerKAT, for example, meant that over a thousand bursaries were made available through the SKA South Africa Human Capacity Development programme. Young scientists like Mofokeng and Mbonani have the opportunity to be part of MeerKAT science projects through their studies, using machine learning and other skills that are high in demand in today’s world. This was one of the messages they brought home.

Gaining new skills

“As an Astronomy research student, I have gained skills such as data analysis, mathematical modelling, communication and writing, programming, and teamwork, among others. These are requirements for most companies and institutions. With the unfolding of the 4th Industrial Revolution, such skills sets make young and aspiring scientists the perfect candidates for making the most of future opportunities,” reflected Mofokeng.

Most of the learners said they have never attended a science-outreach event. They were inspired by the young scientists’ stories and nearly half of them said they could see themselves pursuing a career in science. The learners also expressed a strong interest in more events of this kind, as well as mentorship during Grades 11 and 12 from peers at university. They asked about the salaries earned by astronomers, how long the studies take, and where astronomers are working in South Africa.

This initiative, started by two bright young scientists, hopefully marks the beginning of many more events of this kind. Mofokeng and Mbonani are already planning what to do on their next trip home.

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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