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28 March 2019 | Story Thabo Kessah | Photo Thabo Kessah
Teboho Mofokeng
Postgraduate Student Council and SRC member, Teboho Mofokeng, says one degree is not enough.

Postgraduate studies play a crucial and critical role in the missions of our universities. They also contribute to the key and central mandate of the university – knowledge production, the dissemination, and application thereof.

The Campus Principal, Dr Martin Mandew, expressed this view during a welcoming function for postgraduate students on the Qwaqwa Campus. “Our Postgraduate School is the gateway that enables entry into higher degrees and qualifications. It is an extremely valuable resource and support reference point which is at the disposal of the students,” he said.

Postgraduate research and national development

Dr Mandew added that postgraduate research plays a very important role in national development, as it develops systematic investigation skills among young graduates for the purpose of making a contribution to what he termed ‘the national system of innovation’. “It also ensures that the country is competitive and enables the generation of knowledge that is responsive to societal needs, among others,” he said.

“Doing postgraduate studies is not easy,” he added. “Challenges that postgraduate students have to contend with, include funding and financial problems; lack of equipment; inadequate library facilities; access to research materials, and many more,” Dr Mandew said.

Support broadens knowledge and skills

In detailing the services offered by the Postgraduate School, the Director, Prof Witness Mudzi, assured students that they would experience an enabling environment to excel in the pursuit of their academic quests. “We will provide additional support to that provided by facilities and departments in the form of workshops, courses, and other presentations, which will equip the students with the requisite skills for successful completion of their postgraduate education.”

“The workshops and courses we offer are aimed at broadening your knowledge of research processes and methods. This would then positively influence throughput, publications, and the quality of research produced,” Prof Mudzi said to a packed venue.

Speaking on behalf of the SRC and the Postgraduate Student Council, Chairperson Teboho Mofokeng said that the event was held at a time when final-year students were asking themselves if it was worth continuing with postgraduate studies. “Do not take the decision to continue with your postgraduate studies lightly,” he said. “We work in a knowledge economy where specialised skills have significant commercial value. This means that in today’s competitive job market, it is often not enough to have only one degree,” said Mofokeng, a beneficiary of the school’s Mentorship Programme and master’s student specialising in Parasitology.

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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