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12 March 2020 | Story Thabo Kessah | Photo Thabo Kessah
Japan UFS Afromontane Research Unit research collaboration
Dr Melissa Hansen (left) with ARU guest researchers. They are, from the left: Gema Carlota Cubelos Perez, Emilie Jones, Ven Paolo Valenzuela, Kanako Matsuyama (International Christian University), and Dr Kudo Shogo.

Research ties between the University of the Free State, the University of Tokyo, and the International Christian University strengthened when the Japanese scholars visited the Afromontane Research Unit (ARU) on the Qwaqwa Campus. 

“The visiting delegation is part of the larger research group on sustainability studies that has been sharing research expertise with the Afromontane Research Unit’s researchers over the past three years,” said Dr Kudo Shogo, Assistant Professor from the University of Tokyo’s Graduate Programme in Sustainability Science – Global Leadership Initiative (GPSS-GLI).

Entrepreneurship in Qwaqwa
“Our focus this time is on entrepreneurs who have had exposure to megacities such as Johannesburg and Cape Town, and who are finding themselves back in places like Qwaqwa. We have discovered that they actually find Qwaqwa more resourceful than when they left. Two to three years of unstable living in the cities gave them a fresh view to see the many opportunities in Qwaqwa and they then start their businesses. Talking to the Qwaqwa entrepreneurs has been a great learning experience for all of us,” he added.

The visiting scholars conducted interviews with 10 local entrepreneurs to get a sense of how they use entrepreneurship for sustainability purposes.

“We are pleased by the local people’s understanding that local problems require local solutions. I would really like to contribute to these people’s understanding of how these solutions fit the problems better than solutions that come from outside. We have quite a number of voices talking about empowering Qwaqwa, with the emphasis on creating jobs for Qwaqwa, solving the problems that Qwaqwa is facing. I have found education to be a unifying factor through tutoring, after-school classes, mentorship, and the personal imperative of sharing,” said Emilie Jones, originally from the United States of America and now studying for a master’s degree in Sustainability Science focusing on water supply and resources.

Education and arts empower communities
“Most of the entrepreneurs we spoke to have experience of the big cities. For them, Qwaqwa is very close to the heart and is home. There are challenges, but they are doing their best to empower their community with ideas and skills from the big cities. They provide services such as education and arts to empower the community to come up with a local identity,” said a PhD candidate, Ven Paolo Valenzuela from the Philippines. 

“I was impressed with the people who realise the opportunities to identify problems and even come up with solutions themselves. A lot of communities can learn from this,” said Gema Carlota Cubelos Perez, a PhD candidate originally from Spain.

Their host, Dr Melissa Hansen, Lecturer from the Department of Geography, said the visit was part of the bigger study on migration and sustainable development. “This was a Global Field Exercise (GFE) for teaching research methods in the field. We found that Qwaqwa is overflowing with potential for entrepreneurship in a wide variety of fields and that there is a strong, vibrant network of young individuals brimming with talent. We are learning from each other, as Akita City in Japan and Qwaqwa are similar in more ways than one,” she said.

One of the entrepreneurs, Refiloe Seekane, is a self-taught fashion designer, choreographer, and event coordinator. “The interview has actually made me realise the gaps we have for business opportunities in Qwaqwa and the importance of implementing some of the projects I have been planning for years,” said Seekane, a second-year Education student and CEO of Evomind.


News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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