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13 March 2020 | Story Leonie Bolleurs | Photo Leonie Bolleurs
Team from the UFS Microbiology department
From the Department of Microbiology and Biochemistry, were from the left, front: Dr Mariana Erasmus, Prof Martie Smit, Samantha McCarlie; back: Dr Carmien Tolmie; Samantha McCarlie, Prof Dirk Opperman, and Prof Robert Bragg. They believe publishing in high-impact factor journals reflects the quality of research delivered by the department.

Researchers in the Department of Microbial, Biochemical and Food Biotechnology at the University of the Free State (UFS) published their work in four impact factor eleven journals in 2019/2020, and a fifth was accepted for publication in a journal with impact factor twelve in 2020. 

Two articles were published in Nature Communications, one in Drug Resistance Updates and one in Natural Product Reports. A fifth article is already available as an accepted article at Angewandte Chemie. Researchers in the department work on very diverse topics, as reflected in the titles of these articles: ‘A chemo-enzymatic oxidation cascade to activate C–H bonds with in situ generated H2O2’; ‘Native roles of Baeyer–Villiger monooxygenases in the microbial metabolism of natural compounds’; ‘The genome of a subterrestrial nematode reveals adaptations to heat’; ‘Molecular basis of bacterial disinfectant resistance’; and ‘CYP505E3 – a novel self‐sufficient ω‐7 in‐chain hydroxylase’.

Publishing in journals with a high impact factor is quite an achievement. Publishing in a journal with an impact factor of 3 is considered good and in most fields of study, publishing in journals with an impact factor of 10 or more is regarded as excellent. Impact factors are used to measure the importance of a journal by counting the number of times articles were cited in a certain time period. 

According to Prof Martie Smit, Head of the department, this is a reflection of the quality of research delivered by the department. “It is difficult and takes a lot of time and resources to publish in such high-impact journals.”

Contributing to their success in the department, is the work of their collaborators as well as the quality international postdoctoral researchers the department manages to attract with their emphasis on quality research.

Another highlight in the publication of these articles was that members of the department were corresponding authors of four of the five articles – meaning that the research was conducted in and driven from their laboratories, with UFS researchers taking primary responsibility for the preparation of the manuscripts and communicating with the editors of the journals.

Impacting society

Besides publishing in journals with high impact factors, these researchers are also making a difference to society. Prof Robert Bragg contributed to the study focusing on quality disinfectants. 

We are all aware of the danger of developing resistance to antibiotics. According to Prof Bragg, it is estimated that by 2050, 25 million people could be dying from antibiotic resistance-related bacterial infections per year. He says one of the best options to control diseases – not only bacterial diseases, but also viral diseases such as the Covid-19 outbreak – is good biosecurity and the use of good-quality disinfectants.

Researchers working on this study are trying to understand the development of resistance in bacteria to disinfectants. “This research group is currently investigating the ways in which bacteria become resistant to different high-quality disinfectants. The aim of this work is to discover new methods of resistance and then try to prevent bacteria from becoming resistant to commonly used disinfectants. One of the first aspects that needs investigation is to understand the methods of transfer of genetic information between bacteria. This work formed the basis of the review article written with master’s student Samantha McCarlie on transfer of genes that could code for disinfectant resistance in bacteria,” says Prof Bragg.

Studies about a nematode species discovered 1,3 km deep in a gold mine in Welkom and its ability to survive in extreme environments, made headlines about nine years ago. More extensive research has been performed on deep-space exploration of nematodes surviving extreme environments and were published in Nature Communications. Dr Mariana Erasmus, Assistant Director in the department and Technology Innovation Agency/UFS Saense Platform manager, says the study published in 2019 reveals these nematodes’ adaptation to heat and heat tolerance in an unusual ecosystem isolated from the surface biosphere. More studies on this can help humans learn how to adapt to a warming climate. 

TIA is an agency of the Department of Science and Innovation.

Three of the articles are from the Biocatalysis and Structural Biology group of Prof Dirk Opperman, Prof Martie Smit, and Dr Carmien Tolmie. Biocatalysis is a form of green chemistry that aims to produce chemicals in an environmentally friendly and sustainable manner. The research of the group focuses on using enzymes (proteins performing specialised chemical reactions) to insert an oxygen atom at a specific position in a starting material. Such reactions are difficult to perform using purely organic chemistry. 

The end products are value-added compounds of interest to, among others, the flavour and fragrance industry, which place a high premium on natural products. The work on the novel in‐chain hydroxylase was also patented internationally, because it can be used for the synthesis of a valuable flavour compound.
 
More to come

Besides the commitment of the team in Microbiology, it took multiple institutions, dedicated postdoctoral students, as well as time and money to publish this number of articles in high-impact journals in just over a year. With its 100-plus researchers varying from student researchers to NRF-rated scientists, everyone in the Department of Microbial, Biochemical and Food Biotechnology strives to produce high-quality research. 

And they promise, there is more to come. Watch this space …

News Archive

UFS to investigate implementation of quality-monitoring system for SA food industry
2006-02-07

Some of the guests who attended the workshop were from the left Prof James du Preez (Chairperson: Department of Biotechnology at the UFS); Prof Lodewyk Kock (Head: South African Fryer Oil Initiative (SAFOI) at the UFS)); Mrs Ina Wilken (Chairperson: South African National Consumer Union (SANCU)); Prof Herman van Schalkwyk (Dean: Faculty of Natural and Agricultural Sciences at the UFS) and Mr Joe Hanekom (Managing Director of Agri Inspec).
Photo: Stephen Collet
 

UFS to investigate implementation of quality-monitoring system for SA food industry

The University of the Free State (UFS) will be investigating the implementation of a quality-monitoring service for the South African food industry. 

This was decided during a workshop to discuss the external quality monitoring in the edible oil industry of South Africa, which was recently held at the UFS.

Major role players in the fast-food sector like Nando's, Spur, Captain
Dorego's, King Pie Holdings, Black Steer Holdings, etc and various oil
distributors like Felda Bridge Africa, Refill Oils, PSS Oils and Ilanga Oils attended
the workshop. Also present was Mrs Ina Wilken, Chairperson of the South African National Consumer Union (SANCU) and key-note speaker of this workshop. She represented the consumer.  

These role players all pledged their support to the implementation of this quality- monitoring system for the whole food industry. 

The decision to implement this system follows the various malpractices reported in the press and on TV concerning food adulteration (eg the recent Sudan Red Scare), misrepresentation (eg olive oil scandal exposed in 2001) and the misuse of edible frying oils by the fast-food sector. 

“One of the basic rights of consumers is the right to safe food. Consumers must be protected against foods and food production processes which are hazardous to their health. Sufficient guarantee of the safety of all food products and food production processes should be implemented. It does not help to have adequate food standards and legislation and there is no manpower to do the necessary investigation or monitoring,” said Mrs Wilken.

The South African Fryer Oil Initiative (SAFOI), under the auspices of the UFS Department of Microbial, Biochemical and Food Biotechnology, currently monitors edible oils in the food industry and makes a seal of quality available to food distributors.

“Last week’s decision to implement the quality-monitoring system implies that we will now be involving also other departments in the UFS Faculty of Natural and Agricultural Sciences who are involved in various aspects of the food chain in an endeavor to implement this quality monitoring system,” said Prof Herman van Schalkwyk, Dean:  Faculty of Natural and Agricultural Sciences at the UFS and one of the main speakers at the workshop.

Prof van Schalkwyk said that the main aim of such a system will be to improve the competitiveness of the South African food industry.  “It is clear that the role players attending the workshop are serious about consumer service and that they agree that fraudulent practice should be monitored and corrected as far as possible.  Although some of the food outlets have the capacity to monitor the quality of their food, it may not seem to the consumer that this is an objective process.  The proposed external monitoring system would counteract this perception amongst consumers,” said Prof van Schalkwyk.

The workshop was also attended by representatives from SAFOI and Agri Inspec, a forensic investigation company collaborating with inter-state and government structures to combat fraud and international trade irregularities.

Agri Inspec has been working closely with SAFOI for a number of years to test the content of edible oils and fats.  “Extensive monitoring and control actions have been executed in the edible oil industry during the past four years to ensure that the content and labeling of oil products are correct.  Four years ago almost 90% of the samples taken indicated that the content differed from what is indicated on the label.  This has changed and the test results currently show that 90% of the products tested are in order. However, to maintain this quality standard, it is necessary that quality monitoring and educational campaigns are continuously performed,” said Mr Joe Hanekom, Managing Director of Agri Inspec. 

“The seal of quality presented by SAFOI should also be extended to include all the smaller oil containers used by households,” Mrs Wilken said.

The SAFOI seal of quality is currently displayed mainly on some oil brands packed in bigger 20 liter containers, which include sunflower oil, cottonseed oil, palm oil etc which are used by restaurants and fast food outlets.  “Any oil type is eligible to display the seal when meeting certain standards of authenticity.  In order to display the seal, the distributor must send a sample of each oil batch they receive from the manufacturer to SAFOI for testing for authenticity, ie that the container’s content matches the oil type described on the label. This is again double checked by Agri Inspec, which also draws samples countrywide from these certified brands from the end-user (restaurant or fast food outlets). If in breach, the seal must be removed from the faulty containers,” said Prof Lodewyk Kock, Head of SAFOI.

“It should however be taken into account that oils without a seal of quality from the UFS can still be of high quality and authentic. Other external laboratories equipped to perform effective authenticity tests may also be used in this respect,” said Prof Kock.

“It is also important to realise that any oil type of quality such as sunflower oil, cottonseed oil, palm oil etc can be used with great success in well controlled frying processes,” he said.

Further discussions will also be held with the Department of Health, the SA National Consumer Union and Agri Inspec to determine priority areas and to develop the most effective low-cost monitoring system.

More information on the UFS oil seal of quality and oil use can be obtained at www.uovs.ac.za/myoilguide

Media release
Issued by: Lacea Loader
Media Representative
Tel:   (051) 401-2584
Cell:  083 645 2454
E-mail:  loaderl.stg@mail.uovs.ac.za
6 February 2006

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