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16 October 2020 | Story Leonie Bolleurs | Photo Supplied
Kyla Dooley, runner-up in this year’s Three-minute thesis competition, wants to pursue a career working alongside police enforcement, using her knowledge of forensics to solve criminal cases and convict perpetrators.

When rapes and sexual assaults are committed, DNA evidence can play a large role in convicting the offenders. DNA evidence collected from sexual crimes can, according to Kyla Dooley, often be tricky to analyse.

Kyla has just completed her master’s degree, specialising in Forensic Genetics, at the University of the Free State (UFS). She not only thrives in this field – graduating at the top of the Faculty of Natural and Agricultural Sciences in 2018 when she was awarded the Dean’s Medal – but her work also brought her the runner-up position in this year’s Three-minute thesis competition. 

She talked about her research on the use of male-specific DNA in the analysis of DNA evidence collected after crimes of a sexual nature have been committed.

Explaining her research, Kyla elaborates: “In most cases, the victim is female, while the offender is male. Therefore, the evidence is often a mixture of male and female DNA and this can make it difficult to analyse the male DNA and match it to a male suspect.”

She believes the solution to this is to target male-specific DNA in analysis. “This eliminates all female DNA and simplifies the process,” says Kyla.

“Unfortunately, male-specific DNA technology is not currently used in South Africa, because the DNA regions tested to date haven’t shown much success in distinguishing between males in our population,” Kyla points out.

“The goal is now to use DNA evidence, to match it to a suspect, and have the confidence that it came from him and only him. Or else defence lawyers could argue that it came from someone else in the population,” she says.

Improving DNA evidence

Therefore, Kyla’s research focused on evaluating a new group of male-specific DNA regions, which are to be tested yet, to see if it would be a viable option for use in South Africa. 

“I achieved this by collecting DNA samples from men on campus, processing them to obtain DNA profiles, and then determining how well these regions can distinguish between the men. The results of my research demonstrate the potential of these DNA regions to improve the use of DNA evidence when investigating sexual assaults in South Africa,” says Kyla.

She believes her study can play a role in increasing the conviction rate of sexual offenders, which could lead to a reduction in South Africa’s alarmingly high rape statistic. 

“Everyone in South Africa is affected by this horrific crime in some way or another, so the benefits of this would be widespread,” she says.

Solving crimes

Although Kyla will one day pursue further studies, she is ready for the next stage in her life. “I am in the process of applying for jobs and getting ready to dive into the real world. I’ll definitely be pursuing a career working alongside police enforcement to solve criminal cases and convict perpetrators of such crimes. Working for the NYPD in the USA or Scotland Yard in the UK is the ultimate dream job,” she says.

“I chose my field not only because the forensics world absolutely fascinates me, but also because I want to make a difference. I want to play a role in getting justice for those affected by violent crimes. One simple process in a forensic scientist’s everyday routine could be a life changer for a victim of crime,” believes Kyla.

 

 


News Archive

Researcher shares platform with Nobel Laureate at conference on nanomedicine
2013-01-10

Prof. Lodewyk Kock at the Everest viewpoint with Mount Everest behind him.
10 January 2013

Profs. Lodewyk Kock and Robert Bragg from the Department of Microbial, Biochemical and Food Biotechnology at the University of the Free State (UFS) both presented lectures at the first International Conference on Infectious Diseases and Nanomedicine that was held in Kathmandu, Nepal, late last year.

At the conference, also attended by senior representatives from the International Union of Microbiological Societies (IUMS), Prof. Kock delivered one of the two opening lectures, titled: Introducing New Nanotechnologies to Infectious Diseases (the other opening lecture was presented by Nobel Laureate, Prof. Barry J. Marshal). Prof. Kock also participated in the farewell address.

In two excellent lectures, Prof. Bragg spoke on Bacteriophages as potential treatment option of antibiotic-resistant bacteria, and on Bacterial resistance to quaternary ammonium compounds.

For Prof. Kock this very first conference on infectious diseases and nanomedicine was followed by a very exciting yeast research excursion through the Mount Everest Highway which winds through the villages of the Sherpa tribe.

He describes his journey: “The Mount Everest Highway is a rough road stretching through hills and glacial moraines of unfamiliar altitudes and cold temperatures. Throughout the journey I had to take care of not contracting altitude sickness which causes severe headaches and dizziness.

“The only way of transport is on foot, on long-haired cattle called Yaks, donkeys and by helicopter. After flying by plane from Kathmandu (the capital of Nepal), I landed at Lukla, regarded as the most dangerous airport in the world due to its short elevated runway and mountainous surroundings. From Lukla, the land of the Sherpa, I walked (trekked) with my Sherpa guide and porter (carrier) along the Everest Highway surrounded by various Buddhist Mani scripture stands, other Buddhist representations and many spectacular snow-tipped mountains of more than 6 000 m above sea level. Of these, the majestic mountain called Ama Dablam (6 812 m), the grand 8 516 m high peak of Lhotse and to its left the renowned Mount Everest at 8 848 m in height, caught my attention.

“Dwarfed by these mountain peaks on the horizon, I passed various villages until I eventually reached the beautiful village called Namche Bazar, the heart of the Khumbu region and hometown of the Sherpa. This took three days of up to six hours walking per day, while I spent the nights at the villages of Phakding and Monjo. From there I walked along the Dudh Kosi River which stretches towards Mount Everest, until I reached the high altitude Everest viewpoint – the end of my journey, after which I trekked back to Lukla to return to Kathmandu and South Africa.

“This expedition is the first exploration to determine the presence of yeasts in the Everest region. Results from this excursion will be used in collaborative projects with local universities in Nepal that are interested in yeast research.”

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