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16 October 2020 | Story Leonie Bolleurs | Photo Supplied
Kyla Dooley, runner-up in this year’s Three-minute thesis competition, wants to pursue a career working alongside police enforcement, using her knowledge of forensics to solve criminal cases and convict perpetrators.

When rapes and sexual assaults are committed, DNA evidence can play a large role in convicting the offenders. DNA evidence collected from sexual crimes can, according to Kyla Dooley, often be tricky to analyse.

Kyla has just completed her master’s degree, specialising in Forensic Genetics, at the University of the Free State (UFS). She not only thrives in this field – graduating at the top of the Faculty of Natural and Agricultural Sciences in 2018 when she was awarded the Dean’s Medal – but her work also brought her the runner-up position in this year’s Three-minute thesis competition. 

She talked about her research on the use of male-specific DNA in the analysis of DNA evidence collected after crimes of a sexual nature have been committed.

Explaining her research, Kyla elaborates: “In most cases, the victim is female, while the offender is male. Therefore, the evidence is often a mixture of male and female DNA and this can make it difficult to analyse the male DNA and match it to a male suspect.”

She believes the solution to this is to target male-specific DNA in analysis. “This eliminates all female DNA and simplifies the process,” says Kyla.

“Unfortunately, male-specific DNA technology is not currently used in South Africa, because the DNA regions tested to date haven’t shown much success in distinguishing between males in our population,” Kyla points out.

“The goal is now to use DNA evidence, to match it to a suspect, and have the confidence that it came from him and only him. Or else defence lawyers could argue that it came from someone else in the population,” she says.

Improving DNA evidence

Therefore, Kyla’s research focused on evaluating a new group of male-specific DNA regions, which are to be tested yet, to see if it would be a viable option for use in South Africa. 

“I achieved this by collecting DNA samples from men on campus, processing them to obtain DNA profiles, and then determining how well these regions can distinguish between the men. The results of my research demonstrate the potential of these DNA regions to improve the use of DNA evidence when investigating sexual assaults in South Africa,” says Kyla.

She believes her study can play a role in increasing the conviction rate of sexual offenders, which could lead to a reduction in South Africa’s alarmingly high rape statistic. 

“Everyone in South Africa is affected by this horrific crime in some way or another, so the benefits of this would be widespread,” she says.

Solving crimes

Although Kyla will one day pursue further studies, she is ready for the next stage in her life. “I am in the process of applying for jobs and getting ready to dive into the real world. I’ll definitely be pursuing a career working alongside police enforcement to solve criminal cases and convict perpetrators of such crimes. Working for the NYPD in the USA or Scotland Yard in the UK is the ultimate dream job,” she says.

“I chose my field not only because the forensics world absolutely fascinates me, but also because I want to make a difference. I want to play a role in getting justice for those affected by violent crimes. One simple process in a forensic scientist’s everyday routine could be a life changer for a victim of crime,” believes Kyla.

 

 


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Well-established root system important for sustainable production in semi-arid grasslands
2015-02-24

Plot layout where production and root studies were done
Photo: Supplied

The importance of a well-established root system for sustainable production in the semi-arid grasslands cannot be over-emphasised.

A study of Prof Hennie Snyman from the Department of Animal and Wildlife and Grassland Sciences at the University of the Free State is of the few studies in which soil-water instead of rainfall has been used to estimate above- and below-ground production of semi-arid grasslands. “In the past, plant ecological studies have concentrated largely on above-ground parts of the grassland ecosystem with less emphasis on root growth. This study is, therefore, one of the few done on root dynamics in drier areas,” said Prof Snyman.

The longevity of grass seeds in the soil seed bank is another aspect that is being investigated at present. This information could provide guidelines in grassland restoration.

“Understanding changes in the hydrological characteristics of grassland ecosystems with degradation is essential when making grassland management decisions in arid and semi-arid areas to ensure sustainable animal production. The impact of grassland degradation on productivity, root production, root/shoot ratios, and water-use efficiency has been quantified for the semi-arid grasslands over the last 35 years. Because of the great impact of sustainable management guidelines on land users, this study will be continuing for many years,” said Prof Snyman.

Water-use efficiency (WUE) is defined as the quantity of above- and/or below-ground plant produced over a given period of time per unit of water evapotranspired. Sampling is done from grassland artificially maintained in three different grassland conditions: good, moderate, and poor.

As much as 86, 89 and 94% of the roots for grasslands in good, moderate and poor conditions respectively occur at a depth of less than 300 mm. Root mass is strongly seasonal with the most active growth taking place during March and April. Root mass appears to be greater than above-ground production for these semi-arid areas, with an increase in roots in relation to above-ground production with grassland degradation. The mean monthly root/shoot ratios for grasslands in good, moderate, and poor conditions are 1.16, 1.11, and 1.37 respectively. Grassland degradation lowered above- and below-ground plant production significantly as well as water-use efficiency. The mean WUE (root production included) was 4.79, 3.54 and 2.47 kg ha -1 mm -1 for grasslands in good, moderate, and poor conditions respectively.

These water-use efficiency observations are among the few that also include root production in their calculations.

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