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16 October 2020 | Story Leonie Bolleurs | Photo Supplied
Kyla Dooley, runner-up in this year’s Three-minute thesis competition, wants to pursue a career working alongside police enforcement, using her knowledge of forensics to solve criminal cases and convict perpetrators.

When rapes and sexual assaults are committed, DNA evidence can play a large role in convicting the offenders. DNA evidence collected from sexual crimes can, according to Kyla Dooley, often be tricky to analyse.

Kyla has just completed her master’s degree, specialising in Forensic Genetics, at the University of the Free State (UFS). She not only thrives in this field – graduating at the top of the Faculty of Natural and Agricultural Sciences in 2018 when she was awarded the Dean’s Medal – but her work also brought her the runner-up position in this year’s Three-minute thesis competition. 

She talked about her research on the use of male-specific DNA in the analysis of DNA evidence collected after crimes of a sexual nature have been committed.

Explaining her research, Kyla elaborates: “In most cases, the victim is female, while the offender is male. Therefore, the evidence is often a mixture of male and female DNA and this can make it difficult to analyse the male DNA and match it to a male suspect.”

She believes the solution to this is to target male-specific DNA in analysis. “This eliminates all female DNA and simplifies the process,” says Kyla.

“Unfortunately, male-specific DNA technology is not currently used in South Africa, because the DNA regions tested to date haven’t shown much success in distinguishing between males in our population,” Kyla points out.

“The goal is now to use DNA evidence, to match it to a suspect, and have the confidence that it came from him and only him. Or else defence lawyers could argue that it came from someone else in the population,” she says.

Improving DNA evidence

Therefore, Kyla’s research focused on evaluating a new group of male-specific DNA regions, which are to be tested yet, to see if it would be a viable option for use in South Africa. 

“I achieved this by collecting DNA samples from men on campus, processing them to obtain DNA profiles, and then determining how well these regions can distinguish between the men. The results of my research demonstrate the potential of these DNA regions to improve the use of DNA evidence when investigating sexual assaults in South Africa,” says Kyla.

She believes her study can play a role in increasing the conviction rate of sexual offenders, which could lead to a reduction in South Africa’s alarmingly high rape statistic. 

“Everyone in South Africa is affected by this horrific crime in some way or another, so the benefits of this would be widespread,” she says.

Solving crimes

Although Kyla will one day pursue further studies, she is ready for the next stage in her life. “I am in the process of applying for jobs and getting ready to dive into the real world. I’ll definitely be pursuing a career working alongside police enforcement to solve criminal cases and convict perpetrators of such crimes. Working for the NYPD in the USA or Scotland Yard in the UK is the ultimate dream job,” she says.

“I chose my field not only because the forensics world absolutely fascinates me, but also because I want to make a difference. I want to play a role in getting justice for those affected by violent crimes. One simple process in a forensic scientist’s everyday routine could be a life changer for a victim of crime,” believes Kyla.

 

 


News Archive

Good quality wheat essential for bread production
2016-11-29

Description: Robbie Lindeque Tags: Robbie Lindeque 

Robert Lindeque, wheat breeder at the ARC
Small Grain Institute in Bethlehem.
Photo: Supplied

“Wheat quality, specifically grain protein, is of the most crucial components determining the profitability of wheat farmers.”

This is according to Robbie Lindeque, wheat breeder at the ARC Small Grain Institute in Bethlehem. As a wheat breeder, one of his primary aims is to make a contribution to sustainable wheat production in the inland of South Africa.

A closer analysis of bread wheat protein

With his PHD thesis, "Protein quality versus quantity in South African commercial bread wheat cultivars”, Lindeque answered critical questions regarding the South African wheat industry. The major question of his PhD, which he received on 30 June 2016, was whether protein quality could compensate for protein quantity as a measure of bread quality in South Africa.

The three main wheat-producing areas in South Africa, the dryland summer rainfall region (Free State), dryland winter rainfall region (Western Cape), and the cooler irrigation regions (Northern Cape), were used as a starting point for the study.

Proteins are essential for the baking of good quality bread. Worldwide, the utilisation of wheat flour shipments in the baking industry is determined by the protein proportion of the shipment.

Lindeque says the aim of his thesis was to determine whether a closer analysis of bread wheat protein would provide a better indication of good or bad bread quality. “The conclusion from this study was that both protein quantity and protein quality from all three production areas in South Africa varies constantly in accuracy regarding the estimation of bread volume, mainly as a result of environmental factors,” says Lindeque.

Results relevant to the wheat industry

In 2012, application was made to the Winter Cereal Trust for funding of the project. After funding was approved – thus making the Winter Cereal Trust the main partner – seed samples were collected from the 2012 and 2013 national cultivar adaptation trials.

“After this, the seed underwent protein and flour analyses, which added a third year to the study, with the fourth year consisting of statistical processing and documenting of the results,” says Lindeque.

Funding by the Winter Cereals Trust contributed to the fact that the study constantly attempted to keep issues and results as relevant as possible to the wheat industry.

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