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06 August 2021 | Story Leonie Bolleurs | Photo Supplied
Prof Carlien Pohl-Albertyn, a successful scientist, is living her dream job, as she gets to pursue her passion for microbiology; a career she wanted to pursue ever since Grade 10.

Prof Carlien Pohl-Albertyn, a professor of Microbiology in the Department of Microbiology and Biochemistry, is the holder of the SARChI Chair in Pathogenic Yeasts at the University of the Free State (UFS). She is leading the Pathogenic Yeast Research Group, studying pathogenic yeast infections and necessary treatment options and bringing hope to many immunosuppressed patients battling HIV/Aids, cancer, diabetics, and other diseases. She also recently co-authored an article on the incidence of fungal infections in COVID-19 patients. 

“This is my dream job, as I get to pursue my passion for microbiology,” says Prof Pohl-Albertyn, who already knew as early as Grade 10 that she wanted to become a microbiologist. “I have always been fascinated by the natural world and have known since childhood that I would become a scientist,” she says. 

An interview with Prof Pohl-Albertyn reveals more about the persons who inspired her, her view on the development of women, and how she approaches her work. 

Is there a woman who inspires you and who you would like to celebrate this Women’s Month, and why?

“The first woman who instilled a love for the biological sciences in me was Miss Steyn, my high school Biology teacher. She had a passion for teaching that inspired me to become a microbiologist.”

Prof Pohl-Albertyn, however, states that there were many other women who constantly inspired her to be a better person. 

“My mother inspires me to take responsibility for my choices; my mother-in-law inspires me to be kinder to others; and my best friends’ mothers inspire me to persevere, even when things are difficult.”

“I am also inspired by my female friends, Ezelle van den Heever, who has shown me that there is always a plan to be made to solve a problem; Trudi O’Neill, who manages to balance high-level research, teaching, and administration with her home life; Alicia Sherriff, from whom I am learning to be more emotionally intelligent; and Janine Allen, who has expanded my horizons and regularly shows me how to look at the world in a completely different way.”

What is your response to current challenges faced by women and available platforms for women development?

“Coming from a fairly male-dominated cultural background, I realise that I have been very privileged to not experience challenges just because I am a woman. I have been able to study and work in a field that values women and men equally and have chosen a very capable and extremely supportive husband, who sees me as an equal.”

She, however, realises that this is not the case for many women, and understands that there are women who were not able to follow their dreams and make their own life choices.

Prof Pohl-Albertyn believes that any opportunity for women to overcome challenges should be encouraged, as a society will ultimately be better if everyone is able to fulfil their greatest potential.

What advice would you give to the 15-year-old you?

This successful scientist, spouse, mother of two sons, daughter, friend, and mentor to many, says that she will advise her 15-year-old self to do everything the way that she has been doing it. “I do not regret anything and would not change my life in any way,” she says. 

What would you say makes you a woman of quality, impact, and care?

“I am a person of quality because of my integrity, work ethic, and commitment to my responsibilities – something that is easy if you enjoy what you do every day.”

“I am a person of impact because of the influence I have on my colleagues, who I always treat with respect, and my students, who I teach to the best of my abilities, and which I hope will serve them well in their careers.”

“I am a person of care because I value the contributions of my colleagues and students to my own growth and development,” remarks Prof Pohl-Albertyn.

News Archive

Nanotechnology breakthrough at UFS
2010-08-19

 Ph.D students, Chantel Swart and Ntsoaki Leeuw


Scientists at the University of the Free State (UFS) made an important breakthrough in the use of nanotechnology in medical and biological research. The UFS team’s research has been accepted for publication by the internationally accredited Canadian Journal of Microbiology.

The UFS study dissected yeast cells exposed to over-used cooking oil by peeling microscopically thin layers off the yeast cells through the use of nanotechnology.

The yeast cells were enlarged thousands of times to study what was going on inside the cells, whilst at the same time establishing the chemical elements the cells are composed of. This was done by making microscopically small surgical incisions into the cell walls.

This groundbreaking research opens up a host of new uses for nanotechnology, as it was the first study ever in which biological cells were surgically manipulated and at the same time elemental analysis performed through nanotechnology. According to Prof. Lodewyk Kock, head of the Division Lipid Biotechnology at the UFS, the study has far reaching implications for biological and medical research.

The research was the result of collaboration between the Department of Microbial, Biochemical and Food Biotechnology, the Department of Physics (under the leadership of Prof. Hendrik Swart) and the Centre for Microscopy (under the leadership of Prof.Pieter van Wyk).

Two Ph.D. students, Chantel Swart and Ntsoaki Leeuw, overseen by professors Kock and Van Wyk, managed to successfully prepare yeast that was exposed to over-used cooking oil (used for deep frying of food) for this first ever method of nanotechnological research.

According to Prof. Kock, a single yeast cell is approximately 5 micrometres long. “A micrometre is one millionth of a metre – in laymen’s terms, even less than the diameter of a single hair – and completely invisible to the human eye.”

Through the use of nanotechnology, the chemical composition of the surface of the yeast cells could be established by making a surgical incision into the surface. The cells could be peeled off in layers of approximately three (3) nanometres at a time to establish the effect of the oil on the yeast cell’s composition. A nanometre is one thousandth of a micrometre.

Each cell was enlarged by between 40 000 and 50 000 times. This was done by using the Department of Physics’ PHI700 Scanning Auger Nanoprobe linked to a Scanning Electron Microscope and Argon-etching. Under the guidance of Prof. Swart, Mss. Swart en Leeuw could dissect the surfaces of yeast cells exposed to over-used cooking oil. 

The study noted wart like outgrowths - some only a few nanometres in diameter – on the cell surfaces. Research concluded that these outgrowths were caused by the oil. The exposure to the oil also drastically hampered the growth of the yeast cells. (See figure 1)  

Researchers worldwide have warned about the over-usage of cooking oil for deep frying of food, as it can be linked to the cause of diseases like cancer. The over-usage of cooking oil in the preparation of food is therefore strictly regulated by laws worldwide.

The UFS-research doesn’t only show that over-used cooking oil is harmful to micro-organisms like yeast, but also suggests how nanotechnology can be used in biological and medical research on, amongst others, cancer cells.

 

Figure 1. Yeast cells exposed to over-used cooking oil. Wart like protuberances/ outgrowths (WP) is clearly visible on the surfaces of the elongated yeast cells. With the use of nanotechnology, it is possible to peel off the warts – some with a diameter of only a few nanometres – in layers only a few nanometres thick. At the same time, the 3D-structure of the warts as well as its chemical composition can be established.  

Media Release
Issued by: Mangaliso Radebe
Assistant Director: Media Liaison
Tel: 051 401 2828
Cell: 078 460 3320
E-mail: radebemt@ufs.ac.za  
18 August 2010
 

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