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27 December 2021 | Story Jóhann Thormählen | Photo Supplied
Annerie
The Kovsie Annerie Dercksen is one of South Africa’s most promising youngsters and climbing the cricketing ladder.

She enjoyed every second of playing with some of her heroes and believes the exposure to international cricket will help her become a better player.

Annerie Dercksen is one of South Africa’s most promising youngsters and climbing the cricketing ladder.

Star from Beaufort West

This second-year Education student from the University of the Free State (UFS), who dreams of playing for the Momentum Proteas, represented the South African Emerging Women’s team three times in 2021.

The star from Beaufort West toured with the side to Bangladesh and also played against Zimbabwe and Thailand in One Day and T20 matches.

According to Dercksen, it is an incredible honour and privilege to be a part of a side.

She soaked up the experience and says everyone was willing to share their knowledge.

“I would have to say, sharing the field with some of my heroes and getting to work with some of the best coaches in the country are some of the highlights.”

She says each tour brought its own challenges and this helped her grow in the way she views and approaches the game.

“In Bangladesh we played against a well-established team in foreign conditions while facing a lot of spinners in spin friendly conditions. Personally, it was quite a challenge and I had to come back and work on some options, especially against spin.”

“Each tour brought its own challenges and this helped me grow in the way I view and approach the game.” - Annerie Dercksen

Coming through the ranks

The all-rounder has come through the ranks. She represented South-Western Districts at school level, played for the South African U19 side and is currently representing the Free State.

But Dercksen didn’t always dream cricket, especially not when playing ‘backyard’ cricket with her brother on the farm.

She didn’t even play for a team at school. “Until a boy from our primary school’s team got sick before a game. A teacher came to class and asked, ‘who can play cricket’, and I put up my hand.”

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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