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14 December 2021 Photo Supplied
For Dr Katleho Tsilo, one of the latest graduates from the University of the Free State (UFS) Faculty of Health Sciences says graduating from medical school is a dream come true for her and her family.

For Dr Katleho Tsilo, one of the newest graduates from the University of the Free State (UFS) Faculty of Health Sciences, graduating from medical school does not only represent the answered prayers, persistence and sheer grace, it is also a dream come true for her and her family.

Dr Tsilo graduated on Monday 13 December 2021 during the UFS’ year-end graduating ceremonies. 

“It still feels unreal that I was able to overcome the greatest challenge that I was presented with. In my entire medical school career, I have faced many great challenges, from not having any kind of funding at all, to initially not being accepted into medicine to really having to fight for my position each and every semester while also fighting with and for my fellow students in the various leadership positions aforementioned. 

“There were moments of weakness where I wished to just quit it all, but with support from the faculty and my family, I managed to hold on. My dream was and still is bigger that any challenge that I have ever encountered,” says Dr Tsilo. 

Had various leadership positions

During her student years, this Botshabelo native engaged in various leadership positions. She also had to overcome numerous obstacles including the imposter syndrome. According to her, coming from a poor background, with very little resources, she sometimes felt insecure about what she could offer and what she is capable of. Being a health worker during the COVID-19 pandemic was also very challenging because of the uncertainty of everything and how dangerous the work environment in the hospital was proving to be.

“I was privileged enough to be elected as part of the MSA (Medical Students Association) in the Academics portfolio, this is where I then came up with the idea of collecting textbook donations and gifting the less privileged students. I was also elected to be part of the Residence Committee of the new Health Sciences Residence, in the Finance Portfolio.”

Can’t imagine doing anything else

Though she did not always want to study medicine, she ultimately decided on this field as she could not imagine doing anything else. She believes it is a calling, as she always wanted to live a life full of purpose and helping other people. Helping put a smile on someone’s face when they are going through illness is enough to change one’s life, explains Dr Tsilo about her decision to study medicine. She will be doing her internship at the Leratong Hospital in Krugersdorp, Gauteng.

Dr Tsilo, who is hoping to go into Health Science Education or Public Health, says her motivation comes from her family background. “Having been surrounded by poverty for the greater part of my life pushed me to become the difference. I was and still am determined to not be another statistic. Another thing that really motivates me is being of some help to someone. I cannot change the whole world at one time but I can surely start with one person at a time. I believe so much in the power of giving, whether knowledge, time or resources So much that I started my own foundation: the Open Hands Foundation.”

According to Dr Tsilo, her journey to graduating was intimidating – having to adjust to being a student was already tough, however adjusting to being a medical student proved to be a whole new level of challenging. It came with a lot of late nights and very early mornings, months without seeing her family and having almost no luxurious pastimes. 

But she persevered because she always had a goal and was determined to reach it. She had a vision to complete this degree and do it in record time and that’s exactly what she did. “Your vision should always fuel your mission,” says Dr Tsilo. 

Besides medicine, her other passions include charity work – something she has been doing right through her student years. 

Passion for charity work 

“I love charity work and if you did not find me studying, that’s what you would find me, doing. Open Hands is where I exercise this passion. I collect clothes, shoes and food items for the less privileged in our communities.
To students struggling and also facing difficulties, I have this simple message: Don’t stop. Don’t stop working hard, don’t stop hoping and dreaming and if you need help of any kind, it is always available. Know that you are not struggling alone, struggle is part of the journey – it always seems impossible but as long as you don’t stop you will eventually reach your destination.”

Prof Lynette van der Merwe, who took up her new position as Academic Head in the Division Health Sciences Education, Faculty of Health Sciences on 1 December 2021, congratulated the new cohort of UFS doctors and reminded them of the three Cs in the MBChB programme in 2021 – courage, conviction and compassion. She also proposed a new “C” for the year ahead: Character.  

“Character is seen in how you treat those who can do nothing for you. It takes time to develop, and only grows through suffering and failing; no shortcuts, no life hacks here. Character makes you seek to be worth knowing, rather than well known. Adam Grant says: ‘Meaning is about making a difference, not having an audience. Your legacy is not how many footprints you leave.  It’s how long they last.’ Wherever life takes you from here, whatever happens to you or through you, do what is right, not easy. Be a person of value, not success.”

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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