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A tale of sheer resistance and of never giving up

12 May 2021 | Story Nonsindiso Qwabe | Photo Supplied

A tale of sheer resistance and of never giving up, is what best describes University of the Free State student Puseletso Moqomo’s academic journey.

From changing studies three times, losing NSFAS funding, and not being able to pay her fees, to working as a cashier at a Bloemfontein filling station to fund her education, Moqomo has seen and done it all, and she says she wouldn’t change a single thing about her journey.

She received her Bachelor of Science degree in Microbiology and Genetics in the Faculty of Natural and Agricultural Sciences during the 2021 April virtual graduation ceremony. When asked what kept her going, she said, “I told myself that I would study hard and obtain my degree; no matter what came my way, I wouldn’t give up. I would be tired and unable to study, but I told my mind that I had to do what I had to do to advance.”

Moqomo first encountered financial exclusion when her application for NSFAS funding was not approved in 2016. She did not have the R6 830 that was required for registration, and therefore had to pause her studies indefinitely. She decided to look for a job to pay her fees, and in June of that year she was employed as a temporary cashier at the Engen filling station at Northridge Mall in Noordhoek. “I was embarrassed and ashamed when I lost my NSFAS funding but giving up was not one of the things on my mind. When I started working, I made it very clear that I didn’t want to be a permanent employee; I simply wanted to work enough to have money to pay my fees.”

Juggling work and school paid off

She saved enough to be able to register again in January 2017, but she had to change degree programmes along the way. “After writing my November exams, I would go back to Engen so that I could save money for the following year’s registration. I would fail my modules but still try again,” she said.

NSFAS continued to pay for the rest of her fees, but in 2020, during her final year, she was told that she had exceeded the number of years she could receive funding. “I began working full time because I knew I might not get NSFAS funding even after appealing, so I would work night shifts from Friday to Sunday, then take a bath at work and go to class on Monday mornings. Through all of this, I told myself that I would pass, and I would pass well.”

Fortunately, after relating her whole story to NSFAS during her appeal, she received funding for her final year – which came on time too, as she had to be laid off work temporarily due to the COVID-19 pandemic. She went back to work again in November 2020 and saved enough money to register for a Postgraduate Certificate in Education (PGCE), which she is currently pursuing. She is also currently completing her teaching practical at Ikaelelo Senior Secondary School, where she matriculated in 2013. “I knew I wanted to continue with my studies, so I worked hard.”

“Giving up is not an option; some things do not come easily – not even a degree. For some it might be easy, but for others there will be hurdles that they will have to overcome, but you have to keep going.”

News Archive

Nanotechnology breakthrough at UFS

2010-08-19

Ph.D students, Chantel Swart and Ntsoaki Leeuw

Scientists at the University of the Free State (UFS) made an important breakthrough in the use of nanotechnology in medical and biological research. The UFS team’s research has been accepted for publication by the internationally accredited Canadian Journal of Microbiology.

The UFS study dissected yeast cells exposed to over-used cooking oil by peeling microscopically thin layers off the yeast cells through the use of nanotechnology.

The yeast cells were enlarged thousands of times to study what was going on inside the cells, whilst at the same time establishing the chemical elements the cells are composed of. This was done by making microscopically small surgical incisions into the cell walls.

This groundbreaking research opens up a host of new uses for nanotechnology, as it was the first study ever in which biological cells were surgically manipulated and at the same time elemental analysis performed through nanotechnology. According to Prof. Lodewyk Kock, head of the Division Lipid Biotechnology at the UFS, the study has far reaching implications for biological and medical research.

The research was the result of collaboration between the Department of Microbial, Biochemical and Food Biotechnology, the Department of Physics (under the leadership of Prof. Hendrik Swart) and the Centre for Microscopy (under the leadership of Prof.Pieter van Wyk).

Two Ph.D. students, Chantel Swart and Ntsoaki Leeuw, overseen by professors Kock and Van Wyk, managed to successfully prepare yeast that was exposed to over-used cooking oil (used for deep frying of food) for this first ever method of nanotechnological research.

According to Prof. Kock, a single yeast cell is approximately 5 micrometres long. “A micrometre is one millionth of a metre – in laymen’s terms, even less than the diameter of a single hair – and completely invisible to the human eye.”

Through the use of nanotechnology, the chemical composition of the surface of the yeast cells could be established by making a surgical incision into the surface. The cells could be peeled off in layers of approximately three (3) nanometres at a time to establish the effect of the oil on the yeast cell’s composition. A nanometre is one thousandth of a micrometre.

Each cell was enlarged by between 40 000 and 50 000 times. This was done by using the Department of Physics’ PHI700 Scanning Auger Nanoprobe linked to a Scanning Electron Microscope and Argon-etching. Under the guidance of Prof. Swart, Mss. Swart en Leeuw could dissect the surfaces of yeast cells exposed to over-used cooking oil.

The study noted wart like outgrowths - some only a few nanometres in diameter – on the cell surfaces. Research concluded that these outgrowths were caused by the oil. The exposure to the oil also drastically hampered the growth of the yeast cells. (See figure 1)

Researchers worldwide have warned about the over-usage of cooking oil for deep frying of food, as it can be linked to the cause of diseases like cancer. The over-usage of cooking oil in the preparation of food is therefore strictly regulated by laws worldwide.

The UFS-research doesn’t only show that over-used cooking oil is harmful to micro-organisms like yeast, but also suggests how nanotechnology can be used in biological and medical research on, amongst others, cancer cells.

Figure 1. Yeast cells exposed to over-used cooking oil. Wart like protuberances/ outgrowths (WP) is clearly visible on the surfaces of the elongated yeast cells. With the use of nanotechnology, it is possible to peel off the warts – some with a diameter of only a few nanometres – in layers only a few nanometres thick. At the same time, the 3D-structure of the warts as well as its chemical composition can be established.

Media Release
Issued by: Mangaliso Radebe
Assistant Director: Media Liaison
Tel: 051 401 2828
Cell: 078 460 3320
E-mail: radebemt@ufs.ac.za
18 August 2010

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