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24 May 2021 | Story Xolisa Mnukwa
The 2021 Kovsie ACT Eco-vehicle race puts students’ sustainable energy and critical thinking skills to the test.

The University of the Free State (UFS) Division of Student Affairs’ (DSA) Director of Student Life, Dr WP Wahl, believes the knowledge and skills that students have gained through participating in the 2021 Eco-vehicle project will position them more optimally in the future world of work. “We are also tremendously grateful for the project funding received from merSETA; without their support, none of this would have been possible,” he remarked.

The Kovsie ACT Eco-vehicle race, in conjunction with the overall programme, was established to encourage students to learn more about the technology and logic behind sustainable energy sources and how this can influence the future global society.

This year’s events witnessed students competing according to their UFS residence teams, with Sonnedou, Legatum, Kestell (SonLeTell); Soetdoring, Beyers Naude, Arista (Soetbeyrista); and Roosmaryn, Kagiso, Karee (Kar-is-myn) ending in first, second, and third place respectively, obtaining the highest scores for the races they competed in.

Anton Calitz, Electrical Engineer in University Estates who was the announcer on the day, described the event as one that exceeded his wildest expectations. “From a sustainable energy point of view, the eco-vehicle race results really turned the tables, with lower energy usage per lap being successfully recorded – as anticipated,” he further added.

Andre van Wyk, Client Liaison Officer of merSETA (Manufacturing, Engineering and Related Services Seta) for the Free State and Northern Cape – as one of the sponsors of the innovative programme – extended warm congratulations to the UFS for hosting an outstanding event. He further applauded the university for its resourcefulness in virtually adding electronic media broadcasts to extend the event to the entire UFS community.

“The Kovsie ACT Eco-vehicle programme was eye-opening and exposed me to the broad field of electronics. It definitely came as a challenge – one I had not anticipated on that level, because at times I couldn’t even see what all the building was leading to, but I just had to put my mind and hands to work – it pushed me to think critically and creatively. I was honoured to have been part of this entire experience and I’m grateful to Anton and his team, as well as the Kovsie ACT office, for being a constant support structure throughout the process, as it was not easy.” 

These were the humble words of Sinegugu Sibisi, a University of the Free State (UFS) student who was part of the 2021 Kovsie ACT Eco-vehicle race, where sustainable energy was at the order of the day.

For more information about the Kovsie ACT eco-vehicle skills programme, email ACT at ACT@ufs.ac.za
 

 

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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