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01 October 2021 | Story Lunga Luthuli and Vicky Simpson | Photo Supplied
Anton Engelbrecht _ Farmovs researcher
Anton Engelbrecht, FARMOVS Bioanalysis Specialist.

“At FARMOVS, we have the opportunity to work with the world’s top pharmaceutical companies, where we form part of the evaluation of labelled and/or conjugated antigens and antibodies that are developed for accurate quantification of endogenous and pharmaceutical compounds. Alternatively, commercially available kits and reagents are also used for the same purpose if the sponsor cannot supply the customised antigens/antibodies. The developed assay methods are put through a rigorous validation assessment to confirm the selectivity, sensitivity, and robustness of the assay,” says Anton Engelbrecht, FARMOVS Bioanalysis Specialist. 

FARMOVS, affiliated to the University of the Free State and operating from the Bloemfontein Campus, is a leading clinical research organisation (CRO) with a unique advantage. As the only on-site ISO15189-accredited and GLP-certified pharmacokinetic laboratory on the African continent, with numerous successful inspections by leading international regulators, it offers the highest quality bioanalytical services in a variety of biological matrices for the development of pharmaceutical products.

Engelbrecht says: “The team of bioanalytical experts thrives on the excitement generated by new discoveries that lead to better treatment of a variety of physiological diseases.”

Advanced technology backed by 47 years of bioanalytical experience

The clinical research organisation prides itself on advanced technology, backed by 47 years of bioanalytical experience. It has developed more than 580 validated analytical methods that adhere to the International Council for Harmonisation and the US Food and Drug Administration (FDA) guidelines. FARMOVS’ analytical methods have been used in more than 3 000 pre-clinical and clinical trials, contributing to the manufacturing of pharmaceutical drugs that are now used by households across the globe.

At FARMOVS, Engelbrecht says, it is a “world filled with novel methods of analysis and subsequent technological integration that expands the horizons of clinical research forming an important part of the discovery and production of new life-saving medicines that is constantly improving the quality of life of people all over the world”.

Engelbrecht says: “New technology and innovation should be the building blocks of any laboratory, and among these are the three fastest sample production members of our Immunochemistry Laboratory team – the STARLet pipettors.”

“We chose the Microlab® STARLet apparatus by Hamilton, because of its ability to perform sample analysis in large quantities at a greater speed by means of robotic pipetting and robotic automated microplate reading, which is a semi-automated process.”

He shared his excitement about improvements in the field of immunoassay development for the purposes of pharmaceutical analysis. This involves the preparation of unique immunoanalytical reagents, analysis of new categories of compounds, methodology, and instrumentation. The most important examples in this field are the continuous development of bead-based immunoassays.

Staying competitive in the industry

Immunoassay methods, such as radioimmunoassay (RIA) and enzyme immunoassay (EIA), among others, are also used at FARMOVS to analyse macromolecules for clients. “The RIA method is used for the determination of several pharmaceutically important compounds in biological fluids. RIA requires a sample containing the antigen of interest, a complementary antibody, and a radiolabelled version of the antigen. To increase the selectivity of an assay, all samples are pre-treated to eliminate high molecular weight endogenous matrix components, including anti-drug antibodies,” explains Engelbrecht.

Although FARMOVS has adequate technology to provide market-related results, the plan is to expand the team to include a multiplex platform that is a sensitive, fully automated immunoassay platform with multiplexing and custom assay capability. “This will pave the way to use an even more sensitive method to quantify biomarkers in the fields of oncology, neurology, cardiology, inflammation, and infectious disease. We aim to remain competitive in our industry, so naturally we must recruit the brightest and most evolved to join the team,” he says.

News Archive

Young researchers are equipped to participate in projects relevant in global context
2017-09-05

 Description: Wheat genomics Tags: bioinformatics, Dr Renée Prins, Department of Plant Sciences, DNA and RNA, data sets 

This group of early career researchers received bioinformatics
training in Worcester in the UK from Dr Diane Saunders of the
John Innes Centre in the UK.
Photo: Supplied

The interdisciplinary field that develops methods and software tools to understand biological data is known as bioinformatics. According to Dr Renée Prins, a research fellow in the Department of Plant Sciences at the University of the Free State, there are few tertiary institutions in South Africa that offer a postgraduate degree in Bioinformatics.

“Most institutions focus either on humans, human diseases, forest trees and their pathogens.  They usually do not have spare capacity to assist researchers, for instance, those working on crops in the agricultural sector,” Dr Prins said.

Big data sets need significant skills

With the advancements made in genomics such as high throughput DNA marker platforms and next-generation sequencing technologies, the data sets biologists have to deal with have grown massively big and cannot be dealt with unless you have significant computer skills.

Dr Prins believes that all young researchers need some level of training in this field to be effective in future. The British Council Researcher Links, being run by the Newton Fund, gives early career researchers across selected partner countries the opportunity to form international connections through fully funded workshops and travel grants. Dr Prins made use of this opportunity and with the assistance of the Department of Research Development at the UFS, she arranged for Dr Diane Saunders of the John Innes Centre in the UK, a bioinformatician of note, to present training to a group of 20 early career researchers in Worcester in the UK.

Providing training with Dr Saunders were two other bioinformaticians from the UK, Dr Burkhard Steuernagel (John Innes Centre) and Dr Robert Davey (Earlham Institute). From the UFS side, Eleanor van der Westhuizen and Dr Henriëtte van den Berg (former UFS academic) acted as mentors, providing guidance on funding opportunities and career development skills.

Participating in projects in a global context
The researchers attending the training came from research institutions or academia, and they work involving plants (predominantly wheat) or plant pathogens. A limited number of participants from the commercial sector, including private South African companies focusing on plant breeding and molecular genetics lab work on agriculturally important crops also benefited from the training. 

“Tertiary institutions in South Africa have the obligation to ensure that young scientists are equipped with bioinformatics skills. If they are not equipped with the necessary skills, they will not be able to participate in research projects that are relevant in a global context,” said Dr Prins. 

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