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01 October 2021 | Story Lunga Luthuli and Vicky Simpson | Photo Supplied
Anton Engelbrecht _ Farmovs researcher
Anton Engelbrecht, FARMOVS Bioanalysis Specialist.

“At FARMOVS, we have the opportunity to work with the world’s top pharmaceutical companies, where we form part of the evaluation of labelled and/or conjugated antigens and antibodies that are developed for accurate quantification of endogenous and pharmaceutical compounds. Alternatively, commercially available kits and reagents are also used for the same purpose if the sponsor cannot supply the customised antigens/antibodies. The developed assay methods are put through a rigorous validation assessment to confirm the selectivity, sensitivity, and robustness of the assay,” says Anton Engelbrecht, FARMOVS Bioanalysis Specialist. 

FARMOVS, affiliated to the University of the Free State and operating from the Bloemfontein Campus, is a leading clinical research organisation (CRO) with a unique advantage. As the only on-site ISO15189-accredited and GLP-certified pharmacokinetic laboratory on the African continent, with numerous successful inspections by leading international regulators, it offers the highest quality bioanalytical services in a variety of biological matrices for the development of pharmaceutical products.

Engelbrecht says: “The team of bioanalytical experts thrives on the excitement generated by new discoveries that lead to better treatment of a variety of physiological diseases.”

Advanced technology backed by 47 years of bioanalytical experience

The clinical research organisation prides itself on advanced technology, backed by 47 years of bioanalytical experience. It has developed more than 580 validated analytical methods that adhere to the International Council for Harmonisation and the US Food and Drug Administration (FDA) guidelines. FARMOVS’ analytical methods have been used in more than 3 000 pre-clinical and clinical trials, contributing to the manufacturing of pharmaceutical drugs that are now used by households across the globe.

At FARMOVS, Engelbrecht says, it is a “world filled with novel methods of analysis and subsequent technological integration that expands the horizons of clinical research forming an important part of the discovery and production of new life-saving medicines that is constantly improving the quality of life of people all over the world”.

Engelbrecht says: “New technology and innovation should be the building blocks of any laboratory, and among these are the three fastest sample production members of our Immunochemistry Laboratory team – the STARLet pipettors.”

“We chose the Microlab® STARLet apparatus by Hamilton, because of its ability to perform sample analysis in large quantities at a greater speed by means of robotic pipetting and robotic automated microplate reading, which is a semi-automated process.”

He shared his excitement about improvements in the field of immunoassay development for the purposes of pharmaceutical analysis. This involves the preparation of unique immunoanalytical reagents, analysis of new categories of compounds, methodology, and instrumentation. The most important examples in this field are the continuous development of bead-based immunoassays.

Staying competitive in the industry

Immunoassay methods, such as radioimmunoassay (RIA) and enzyme immunoassay (EIA), among others, are also used at FARMOVS to analyse macromolecules for clients. “The RIA method is used for the determination of several pharmaceutically important compounds in biological fluids. RIA requires a sample containing the antigen of interest, a complementary antibody, and a radiolabelled version of the antigen. To increase the selectivity of an assay, all samples are pre-treated to eliminate high molecular weight endogenous matrix components, including anti-drug antibodies,” explains Engelbrecht.

Although FARMOVS has adequate technology to provide market-related results, the plan is to expand the team to include a multiplex platform that is a sensitive, fully automated immunoassay platform with multiplexing and custom assay capability. “This will pave the way to use an even more sensitive method to quantify biomarkers in the fields of oncology, neurology, cardiology, inflammation, and infectious disease. We aim to remain competitive in our industry, so naturally we must recruit the brightest and most evolved to join the team,” he says.

News Archive

Researcher uses NRF funding for studies to conserve plant and animal life
2017-04-18

Description: Butterfly Tags: Butterfly

It is difficult to survey all different types of
plants and animals and is therefore necessary to
choose one representative group. Butterflies are
relatively cheap and easy to sample. They are
known to be linked to specific habitats and to
respond to human pressures, such as farming.
Photo: Dr Falko Buschke


Earth is the only planet we know of that contains life. The variety of different plants and animals is remarkable: from the giant whales that swim our oceans, to the tiny mosses that grow on the shaded sides of rocks.  Many of these plants and animals are important to humans. For example, trees provide us with oxygen to breathe, bees pollinate our crops and owls control pests. More importantly though, we can tell a lot about society from the way it cares for nature. Humans are the custodians of the planet and the way we care for nature reflects the way we value life.

Dr Falko Buschke, Lecturer at the Centre for Environmental Management at the University of the Free State, is interested in understanding how the distribution of biodiversity [the variety of living things in nature] in time and space influences the way we should conserve and manage nature.

Earth is losing biodiversity faster than at any time in human history

The planet is losing biodiversity faster than at any time in human history. “There is an urgency to conserve plants and animals before they are lost forever. Nature is complex, so the way we study it should embrace this complexity. We should not rely on limited data on one type of species from one place and assume that it will also apply elsewhere. Instead, it is important that biodiversity research is comprehensive in the types of plants and animals while also considering that ecological and evolutionary processes vary through time and across geographic space,” he said.

To conduct his research, Dr Buschke uses a variety of research tools, including biological data surveyed directly from nature, spatial data from satellite remote sensing and geographic information systems databases, and data generated though custom-built computer simulations.

"There is an urgency to conserve
plants and animals before they
are lost forever."

Field work in the eastern Free State
Although parts of the eastern Free State are considered a global priority for biodiversity conservation, it is mainly privately owned commercial farmland. This means that it is important that plants and animals can survive despite living side by side with agricultural production.

“My project investigates whether the sandstone outcrops, known as inselbergs (island-mountains), are safe havens for plants and animals. Because it is difficult to survey all the different types of plants and animals, it is necessary to choose one representative group. That is where butterflies come in. Butterflies are relatively cheap and easy to sample. They are known to be linked to specific habitats and to respond to human pressures, such as farming,” he said. “Once this butterfly data is collected, it can be linked to satellite information on plant growth patterns. This will provide a clearer picture of whether plants and animals can persist side-by-side with commercial agriculture”.

Dr Buschke has just begun surveys that will carry on until the end of this year. “This 12-month project is funded under the Foundational Biodiversity Information Programme through the South African National Biodiversity Institute (SANBI) and the National Research Foundation (NRF).

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