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13 September 2021 | Story Dr Nitha Ramnath

As a public higher-education institution in South Africa with a responsibility to contribute to public discourse, the University of the Free State (UFS) will be presenting the webinar as part of the Free State Literature Festival’s online initiative, VrySpraak-digitaal. 

The aim of the webinar series is to discuss issues facing South Africa by engaging experts at the university and in South Africa. Some of the topics for 2021 include, among others, reimagining universities for student success; corruption in South Africa – the endemic pandemic; South African politics and the local government elections; and Is South Africa falling apart. In 2020, the webinar series saw the successful participation of leading experts discussing COVID-19 and the crisis facing the country socially, economically, and politically. 

This year, in lieu of the Free State Arts Festival, the UFS will present the webinar virtually over a period of six months. 

Fifth webinar presented on 28 September 2021

A number of surveys have found some degree of vaccine hesitancy among the public. This webinar will clarify why we need to vaccinate against COVID-19 and why vaccines are safe. A major development in the COVID-19 pandemic has been the arrival and distribution of safe and effective vaccines. As the Delta variant of SARS-CoV-2 spreads around the world, the vaccine has proven to be safe and effective enough to prevent severe life-threatening COVID-19 complications. Although vaccines do not fully protect everyone who is vaccinated, nor guarantee zero transmission, a great deal of adherence to other measures is still required. Returning to a new normal routine of life can only happen as more people are vaccinated.


Date: Tuesday, 28 September 2021
Topic: Why vaccinate?
Time: 12:30-14:00
RSVP: Alicia Pienaar, pienaaran1@ufs.ac.za by 24 September 2021 

Facilitator:

Prof Francis Petersen
Rector and Vice-Chancellor, UFS

Panellists:

Prof Adrian Puren
Acting Executive Director
National Institute for Communicable Diseases (NICD)

Dr Nicholas Pearce

Head of Department: Surgery
Faculty of Health Sciences, UFS


Prof Glenda Gray
President and CEO
South African Medical Research Council (SAMRC)

Dr Angelique Coetzee
Chairperson
South African Medical Association (SAMA)


Bios of speakers:

Prof Puren is the newly appointed Acting Executive Director of the NICD since December 2020. He was trained and held a lectureship at the University of the Witwatersrand, before taking on various positions at the NICD. Prof Puren was appointed as Deputy Director and Head of Virology in 1999, and as Head of the Centre for HIV and STIs in 2017.  As Head of Virology, he focused on developing and implementing a range of viral diagnostic platforms in support of the NICD’s EPI surveillance programmes and diagnostic support.

His main interest is in the development of HIV surveillance programmes, with a particular focus on HIV incidence and the use of ‘big data’ to inform surveillance, monitoring, and evaluation. Prof Puren heads the regional and national endpoint diagnostics laboratory for HVTN-supported vaccine and antibody-mediated preventions trials, and he serves as the quality assurance technical manager for the NICD. In this capacity, he has provided support to the National Department of Health’s implementation and quality assurance of HIV rapid testing. Prof Puren serves on various expert bodies, the most recent of which is the South African Lancet Commission on High-Quality Health in the era of Sustainable Development Goals.

Dr Nicholas Pearce

Dr Pearce graduated from the University of the Witwatersrand in 2002, after which he completed his internship at the Universitas Academic Hospital in 2003 and has been in the Free State ever since. He completed his postgraduate training at the University of the Free State and obtained a master’s degree in General Surgery as well as a Fellowship in General Surgery from the College of Surgeons to qualify as a subspecialist in vascular surgery.

Over the years, Dr Pearce has been a consultant in general surgery, a vascular fellow and head of vascular surgery, and is currently the Head of General Surgery at the University of the Free State as well as in the Free State province. He serves on the national Association of Surgeons of South Africa (ASSA), is a member of the Vascular Society of Southern Africa and is an examiner for the College of Surgeons. He also serves on the board of the College of Surgeons as an executive member, is a member of the European Society for Vascular Surgery, and an executive member of the Surgical Research Society of South Africa.

He is responsible for undergraduate, postgraduate, and subspecialist training at the University of the Free State, as well as nationally, and is often an examiner at other institutions throughout South Africa. His publications over the years have been in the surgical field on diverse topics covering the ambit of surgery in South Africa. 

Since the beginning of the COVID-19 pandemic in 2020, he has been instrumental in setting up multiple field and surge facilities throughout the province, as well as several vaccination sites. Dr Pearce has also been involved in multiple studies on COVID-19 over the past year, is currently serving as a provincial task team member for COVID-19 and is also the Universitas COVID-19 task team chair.

Prof Glenda Gray is the President and CEO of the South African Medical Research Council (SAMRC) and former Chair of the Research Committee on COVID-19, providing scientific evidence and experience to the Minister of Health and the National Coronavirus Command Council. 

Prof Gray studied medicine and paediatrics at Wits University, where she remains Full Professor: Research in the School of Clinical Medicine. She is a National Research Foundation A1-rated scientist and is world-renowned for her research on HIV vaccines and interventions to prevent mother to child transmission of HIV. Prof Gray, together with James McIntyre, co-founded and led the globally eminent Perinatal HIV Research Unit at Chris Hani Baragwanath Hospital in Soweto, for which she and McIntyre received the Nelson Mandela Health and Human Rights Award in 2002.

She is co-principal investigator of the National Institutes of Health-funded HIV Vaccine Trials Network (HVTN) and directs the programme in Africa. 

Prof Gray’s accolades include, among others, the Hero of Medicine Award from the International Association of Physicians in AIDS Care, and the Outstanding Africa Scientist Award from the European and Developing Countries Clinical Trials Partnership.

She was named one of Africa’s 50 Most Powerful Women by Forbes, and by TIME as one of the world’s 100 Most Influential People. In 2013, Prof Gray was awarded South Africa’s highest honour, the Order of Mapungubwe. Her qualifications include MBBCh (Wits), FCPaeds (SA), DSc (honoris causa Simon Fraser University), DSc (honoris causa Stellenbosch University), and LLD (honoris causa Rhodes University).


Dr Angelique Coetzee is the National Chair of the South African Medical Association (SAMA) and is leading Pillar 5 on health service delivery of the Presidential Health Summit. She has extensive knowledge of private practice and is a member of various initiatives driving primary healthcare. Over the years, Dr Coetzee held numerous chair and vice chair positions in the SAMA on national and branch level. Dr Coetzee was a member of the National Ministerial Task Team on Military Hospitals in 2013; Chairperson Ministerial Medical Task Team on Internal and External Deployment SANDF 2014, and was elected as Vice Chair of the Medical Parole Advisory Board 2011.
Her credentials include BMedSci and MBChB (University of Pretoria), Post graduate Certificate in Advanced Health Management (CUM LAUDE)  FPD, Post graduate Higher Certificate in Criminal Justice and Forensic Investigations at the Faculty of Law from the University of Johannesburg , . She is currently completing her fraud examiners certificate with the Association of Certified Fraud Examiners (ACFE).


News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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