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16 February 2022 | Story Leonie Bolleurs | Photo Supplied
Unique PhD Journeys
Prof Liezel Lues and her two doctoral students on graduation day. On the left is Dr Modeni Sibande, who is looking forward to ensuring that Public Administration and Management remains relevant to contemporary evolving issues in society. On the right is Dr Maréve Biljohn, who as a student has always shown commitment to do her best in every aspect of her PhD journey.

In nature, one often comes across cool and surreal phenomena. Experiencing rare happenings in the academia is an altogether different encounter. One that Prof Liezel Lues, Professor in the Department of Public Administration and Management at the University of the Free State (UFS), explains as winning the lottery.

Two of Prof Lues’ doctoral students – representing two different institutions – graduated in 2018. Four years later, on the exact same date, 1 March 2022, Drs Maréve Biljohn and Modeni Sibanda will take up their new positions, respectively as Head of the Department of Public Administration and Management at the UFS and Head of the Department of Public Administration at the University of Fort Hare.

 

Social innovation and service delivery

Dr Biljohn, currently Senior Lecturer in the department, did her thesis on the topic: Social innovation and service delivery by local government: a comparative perspective. With work experience in local government, Dr Biljohn had a good idea of the problems that underpin poor service delivery in this sphere of government.

Public participation in integrated development planning: a case study of Buffalo City Metropolitan Municipality, was the title of Dr Sibanda’s thesis. The study revealed how individuals and communities navigate forms of power and raise the critical consciousness of municipal residents, communities, and public officials.

According to Dr Sibanda, his study was motivated by the need to explore how public participation power dynamics influence Integrated Development Planning outcomes.

He believes by doing so, the complexity of how individuals and communities navigate forms of power in public participation platforms and spaces would be unravelled. Unravelling such public participation power dynamics, he says, would raise critical consciousness and address and challenge visible, hidden, and invisible forms of power on these public platforms and spaces. “Often public participation platforms and spaces neglect and ignore the capacity of such spaces to manage the pervasive, complex power dynamics among stakeholders in municipal strategic development planning processes. This focus to my PhD therefore sought to fill that knowledge gap,” adds Dr Sibanda.

Prof Lues says the value link to their research is buoyed in the South African Local Government. “They have both established a niche area that addresses the challenges South African municipalities face,” she adds.


“There is no doubt that they are suitable for the position of head of department at this point.”


Achieving a coveted status in their careers

On experiencing this unique journey, Prof Lues says: “Of all the relations, a relation between a promoter and a student is the most inspiring and admirable one. Any promoter takes the utmost pride when his/her taught students achieve coveted status in their respective careers. To me, it feels like winning the lottery – twice.”

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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