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11 July 2022 | Story Andre Damons | Photo Supplied
Prof Martie Smith and Prof Drik Opperman
Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.

Flavours and fragrances have a wide application in the food, feed, cosmetic, chemical and pharmaceutical sectors. Many flavour compounds are still produced via chemical synthesis or via extraction from plant or animal sources. However, there is increasing interest in their bio-production or the use of flavour compounds of (micro) biological origin. 

One reason for this shift is that chemical synthesis often uses environmentally unfriendly processes. Chemical synthesis usually also produces racemic mixtures with the second enantiomer, mirror image of the looked-for compound, often having undesirable organoleptic properties. Furthermore, the consumer has developed a “chemophobia”-attitude towards synthetic chemical compounds, especially when related to food and home-care products.  This applies even to nature-identical compounds – products that occur in nature but are produced via a non-natural chemical process. Products produced with the use of enzymes or microbes from “natural” substrates can be labelled “natural”. The flavour and fragrance industry thus pay higher prices for such products labelled as “natural”.  

The invention

A University of the Free State (UFS) team, led by Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry are conducting exciting research in this area. They filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.  

The invention relates to a process for the enzymatic in-chain hydroxylation of C12 to C16 fatty acids, alcohols, and alkanes. Hydroxylation of C12 fatty acid and alcohol provides routes for the synthesis of “natural” δ-dodecalactone. The advantage of these routes is that they do not rely on massoia lactones. Massoia lactones are derived from the bark of Massoia trees which grow in Indonesia. Harvesting of the bark kills the trees.  

The cytochrome P450 enzymes (P450s) claimed in this patent are to the inventors’ knowledge the most regioselective enzymes described thus far that can be used for the synthesis of δ-dodecalactone from lauric acid or 1-dodecanol. The approach that the technology takes is to claim cytochrome P450 enzymes that share 70 % amino acid identity to a set of selected P450s for the regioselective hydroxylation of lauric acid and 1-dodecanol to synthesise δ-dodecalactone.

Still in early stage

The current state of development is early stage with the technology only demonstrated in the laboratory on a small scale (100-200 ml). Before the technology can be commercialised the team would need to further improve the regioselectivity and stability of the P450s and proof that the reactions can be scaled up in bioreactors. The technology will probably be delivered as an enzyme (amino acid sequence) with the desired properties. 

There are other research groups working on a synthetic biology approach for the de novo synthesis of δ-dodecalactone from glucose by genetically engineered microbes. It is still unclear how such a process will compare in terms of product yields, economics and environmental impact with the processes proposed by the UFS patent.

If the team had to partner with a commercial company, their first choice would be to work with an established flavour and fragrance company. Another possibility would be the small French flavour and fragrance company that Dr Alizé Pennec, the post-doc and co-inventor who initially discovered the unique P450 activity, is working for.

Please view the videos for more information on patents.

The Vice-Rector: Research and Internationalisation has released two new calls for applications for funding. Academic staff and researchers are encouraged to submit applications for these funds. At this stage we are not accepting projects from Research Fellows. 

The two funds are: 

1.  The Industrial Engagement Fund 
2.  The Intellectual Property Commercialisation Fund

Each fund has its own guidelines and application process. The guidelines are attached. The applications must be filled in on RIMS.

The RIMS application forms can be found through this link

For more information please click the documents below:



News Archive

Researcher takes home gold at international Famelab competition
2017-06-26

Description: Famelab competition Tags: Famelab competition

UFS researcher nabbed a top international award for
her ground-breaking metallurgical research in the UK.
Photo: Supplied

Recently, University of the Free State (UFS) Centre for Environmental Management master’s student, Tshiamo Legoale, was announced the FameLab International champion at the Cheltenham Science Festival in the United Kingdom. She is probing methods to use wheat as a gold hyper-accumulator – or, as she puts it, “grow gold from wheat”. The young researcher made South Africa proud by winning both the audience’s and the judges’ vote.

Coming back home a hero
“Winning was a surprise to me, because all 31 contestants had wonderful research. They all had really good presentations. I’m very grateful for all the support that I received from home. Social media showed me a lot of love and support. When I felt unconfident, they gave me ‘likes’ and that boosted my confidence a bit,” said Legoale about her win.

As South Africa celebrates Youth Month in June, Tshiamo represents hope for thousands of young South Africans to overcome difficult circumstances and follow careers in science.

The human impact is crucial, because Legoale’s win is not only scientific. It is also social and political. As a young female scientist in South Africa, she represented one of three African countries making it to the finals of FameLab, which has grown to one of the largest science communication competitions internationally.

With this in mind, Legoale says it may, in the end, be necessary to balance the needs of communities with the desire to increase yield. “Are we looking to make a fortune or are we looking to put food on the table?” she asks. “These are all things we consider when we conduct such research.”

World-class research from Africa
In South Africa, an estimated 17.7 million tons of gold is wasted. “All this gold was mined out previously, but tiny amounts remain in the dumps,” Legoale explains.

Her research focuses on the uses of wheat as a gold hyper-accumulator, which essentially means wheat plants are used to harvest gold from mine dumps. Simply put, the wheat is planted in the dumps, where enzymes found in the roots react with the gold and the plant absorbs it. The gold is then absorbed by every part of the plant, except the seeds, which means the next harvest can be used for food if need be.

“South Africa's world-champion young scientist, Tshiamo, represents all that is good about this country – brilliant, bright, and set for a fine future. I'm so proud that British Council SA, together with our partners SAASTA and Jive Media Africa, can help her along the way. Huge congratulations to her from all of us – it is a big win for Africa on the world stage,” said Colm McGivern, British Council South Africa Country Director.

The research represents a win on multiple levels. First, there are the obvious potential socio-economic benefits: food production, job creation, and phytomining is more economical than other contemporary mining methods.

Then there is safety. It is a more environmentally friendly practice than methods like heap leaching, carbon-in-leach or carbon-in-pulp. It is also safer for miners themselves, who will not be exposed to dangerous chemicals like mercury, which has been responsible for a great deal of toxicity in mine dumps. And it is safer for those living in the surrounds.

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