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11 July 2022 | Story Andre Damons | Photo Supplied
Prof Martie Smith and Prof Drik Opperman
Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.

Flavours and fragrances have a wide application in the food, feed, cosmetic, chemical and pharmaceutical sectors. Many flavour compounds are still produced via chemical synthesis or via extraction from plant or animal sources. However, there is increasing interest in their bio-production or the use of flavour compounds of (micro) biological origin. 

One reason for this shift is that chemical synthesis often uses environmentally unfriendly processes. Chemical synthesis usually also produces racemic mixtures with the second enantiomer, mirror image of the looked-for compound, often having undesirable organoleptic properties. Furthermore, the consumer has developed a “chemophobia”-attitude towards synthetic chemical compounds, especially when related to food and home-care products.  This applies even to nature-identical compounds – products that occur in nature but are produced via a non-natural chemical process. Products produced with the use of enzymes or microbes from “natural” substrates can be labelled “natural”. The flavour and fragrance industry thus pay higher prices for such products labelled as “natural”.  

The invention

A University of the Free State (UFS) team, led by Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry are conducting exciting research in this area. They filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.  

The invention relates to a process for the enzymatic in-chain hydroxylation of C12 to C16 fatty acids, alcohols, and alkanes. Hydroxylation of C12 fatty acid and alcohol provides routes for the synthesis of “natural” δ-dodecalactone. The advantage of these routes is that they do not rely on massoia lactones. Massoia lactones are derived from the bark of Massoia trees which grow in Indonesia. Harvesting of the bark kills the trees.  

The cytochrome P450 enzymes (P450s) claimed in this patent are to the inventors’ knowledge the most regioselective enzymes described thus far that can be used for the synthesis of δ-dodecalactone from lauric acid or 1-dodecanol. The approach that the technology takes is to claim cytochrome P450 enzymes that share 70 % amino acid identity to a set of selected P450s for the regioselective hydroxylation of lauric acid and 1-dodecanol to synthesise δ-dodecalactone.

Still in early stage

The current state of development is early stage with the technology only demonstrated in the laboratory on a small scale (100-200 ml). Before the technology can be commercialised the team would need to further improve the regioselectivity and stability of the P450s and proof that the reactions can be scaled up in bioreactors. The technology will probably be delivered as an enzyme (amino acid sequence) with the desired properties. 

There are other research groups working on a synthetic biology approach for the de novo synthesis of δ-dodecalactone from glucose by genetically engineered microbes. It is still unclear how such a process will compare in terms of product yields, economics and environmental impact with the processes proposed by the UFS patent.

If the team had to partner with a commercial company, their first choice would be to work with an established flavour and fragrance company. Another possibility would be the small French flavour and fragrance company that Dr Alizé Pennec, the post-doc and co-inventor who initially discovered the unique P450 activity, is working for.

Please view the videos for more information on patents.

The Vice-Rector: Research and Internationalisation has released two new calls for applications for funding. Academic staff and researchers are encouraged to submit applications for these funds. At this stage we are not accepting projects from Research Fellows. 

The two funds are: 

1.  The Industrial Engagement Fund 
2.  The Intellectual Property Commercialisation Fund

Each fund has its own guidelines and application process. The guidelines are attached. The applications must be filled in on RIMS.

The RIMS application forms can be found through this link

For more information please click the documents below:



News Archive

UFS research could light up South African homes
2016-01-21

Reitumetse Maloa, postgraduate student and researcher at the UFS Department of Microbial, Biochemical and Food Biotechnology, is using her research to provide solutions to the energy crises in South Africa.

A young researcher at the university is searching for the solution to South Africa’s energy and electricity problems from a rather unlikely source: cow dung.

“Cow dung could help us power South Africa,” explains Reitumetse Maloa, postgraduate student and researcher at the UFS Department of Microbial, Biochemical and Food Biotechnology.

Reitumetse’s research is trying to understand how the bacteria works that is responsible for producing biogas.

“Biogas can be used for cooking, heating, lighting and powering generators and turbines to make electricity. The remaining liquid effluent can fertilise crops, as it is high in nitrogen, phosphorus and potassium.”

By using cow dung and food waste to produce biogas, we will be able to lower greenhouse gases.

Biogas is produced in a digester - an oxygen-free space in which bacteria break down or digest organic material fed into the system. This process naturally produces biogas, which is mainly a mixture of methane and carbon dioxide.

“Many countries, such as Germany and the United States, have begun generating electricity from cow dung and food waste, through a process known as biogas production. In South Africa, a number of industries, including waste-water treatment facilities and farms, have caught on to this technology, using it to generate heat and to power machines.”

Until recently the world has relied heavily on electricity derived from fossil fuels such as coal, natural gas and oil. Once these fuels have been extracted from underground reservoirs, they are treated or cleaned, transported to power plants and transformed into the electricity that will reach your house. Fossil fuels are considered a ‘dirty’ energy source which gives off greenhouse gases when burned. Those gases are the major contributing factor to climate change.

“We know very little about the interaction of the bacteria inside the biogas digester. To use biogas as a sustainable fuel source, we need to understand and describe the bacteria population and growth dynamics inside the digester to produce biogas optimally. Currently we are testing a variety of feedstock, including bran, maize and molasses, for biogas production potential, as well as optimising the conditions leading to maximum biogas production. We are also exploring the potential to use the effluent as fertiliser on local farms. The ultimate goal is to have biogas systems that will supply our university with clean energy.”


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