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11 July 2022 | Story Andre Damons | Photo Supplied
Prof Martie Smith and Prof Drik Opperman
Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.

Flavours and fragrances have a wide application in the food, feed, cosmetic, chemical and pharmaceutical sectors. Many flavour compounds are still produced via chemical synthesis or via extraction from plant or animal sources. However, there is increasing interest in their bio-production or the use of flavour compounds of (micro) biological origin. 

One reason for this shift is that chemical synthesis often uses environmentally unfriendly processes. Chemical synthesis usually also produces racemic mixtures with the second enantiomer, mirror image of the looked-for compound, often having undesirable organoleptic properties. Furthermore, the consumer has developed a “chemophobia”-attitude towards synthetic chemical compounds, especially when related to food and home-care products.  This applies even to nature-identical compounds – products that occur in nature but are produced via a non-natural chemical process. Products produced with the use of enzymes or microbes from “natural” substrates can be labelled “natural”. The flavour and fragrance industry thus pay higher prices for such products labelled as “natural”.  

The invention

A University of the Free State (UFS) team, led by Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry are conducting exciting research in this area. They filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.  

The invention relates to a process for the enzymatic in-chain hydroxylation of C12 to C16 fatty acids, alcohols, and alkanes. Hydroxylation of C12 fatty acid and alcohol provides routes for the synthesis of “natural” δ-dodecalactone. The advantage of these routes is that they do not rely on massoia lactones. Massoia lactones are derived from the bark of Massoia trees which grow in Indonesia. Harvesting of the bark kills the trees.  

The cytochrome P450 enzymes (P450s) claimed in this patent are to the inventors’ knowledge the most regioselective enzymes described thus far that can be used for the synthesis of δ-dodecalactone from lauric acid or 1-dodecanol. The approach that the technology takes is to claim cytochrome P450 enzymes that share 70 % amino acid identity to a set of selected P450s for the regioselective hydroxylation of lauric acid and 1-dodecanol to synthesise δ-dodecalactone.

Still in early stage

The current state of development is early stage with the technology only demonstrated in the laboratory on a small scale (100-200 ml). Before the technology can be commercialised the team would need to further improve the regioselectivity and stability of the P450s and proof that the reactions can be scaled up in bioreactors. The technology will probably be delivered as an enzyme (amino acid sequence) with the desired properties. 

There are other research groups working on a synthetic biology approach for the de novo synthesis of δ-dodecalactone from glucose by genetically engineered microbes. It is still unclear how such a process will compare in terms of product yields, economics and environmental impact with the processes proposed by the UFS patent.

If the team had to partner with a commercial company, their first choice would be to work with an established flavour and fragrance company. Another possibility would be the small French flavour and fragrance company that Dr Alizé Pennec, the post-doc and co-inventor who initially discovered the unique P450 activity, is working for.

Please view the videos for more information on patents.

The Vice-Rector: Research and Internationalisation has released two new calls for applications for funding. Academic staff and researchers are encouraged to submit applications for these funds. At this stage we are not accepting projects from Research Fellows. 

The two funds are: 

1.  The Industrial Engagement Fund 
2.  The Intellectual Property Commercialisation Fund

Each fund has its own guidelines and application process. The guidelines are attached. The applications must be filled in on RIMS.

The RIMS application forms can be found through this link

For more information please click the documents below:



News Archive

Students receive hands-on crime scene investigation training
2016-09-02

Description: Crime scene investigation training Tags: Crime scene investigation training

Ntau Mafisa, a forensic science honours student
at the UFS, and Captain Samuel Sethunya from
the SAPS Crime Scene Management in
Bloemfontein.
Photo: Leonie Bolleurs

With murder and robbery rates on the rise, the Forensic Science Programme of the Department of Genetics at the University of the Free State is playing a key role in training South Africa’s future crime scene investigators and forensic laboratory analysts.

According to the Institute for Security Studies (ISS), murder and aggravated robbery rates for 2014/2015, as recorded by the South African Police Services (SAPS) have increased. Incidents of murder increased by 4.6% in the period from 2013/2014 to 2014/2015 and aggravated robbery increased by 8.5 % in the same period. The ISS is an African organisation thant enhances human security by providing independent and authoritative research, expert policy advice and capacity building.

Dr Ellen Mwenesongole, a forensic science lecturer at the Department of Genetics, said the university was one of a few universities in South Africa that actually had a forensic science programme, especially starting from undergraduate level.

Crime scene evaluation component incorporated in curriculum
As part of its Forensic Science Honours Programme, the department has, for the first time, incorporated a mock crime scene evaluation component in its curriculum. Students process a mock crime scene and are assessed based on how closely they follow standard operating procedures related to crime scenes and subsequent laboratory analysis of items of possible evidential value.

The mock crime scene forms part of a research project data collection of the honours students. In these projects students utilise different analytical methods to analyse and distinguish between different types of evidence such as hair fibres, cigarette butts, illicit drugs and dyes extracted from questioned documents and lipsticks.

Students utilise different analytical methods to analyse
and distinguish between different types of evidence.

This year, the department trained the first group of nine students in the Forensic Science Honours Programme. Dr Mwenesongole, who received her training in the UK at the University of Strathclyde in Glasgow, Scotland, and Anglia Ruskin University in Cambridge, England, said incorporating a crime scene evaluation component into the curriculum was a global trend at universities that were offering forensic science programmes.

Department of Genetics and SAPS collaborate
It is important to add this component to the student’s curriculum. In this way the university is equipping students not only with theoretical knowledge but practical knowledge on the importance of following proper protocol when collecting evidence at crime scenes and analysing it in the laboratory to reduce the risk of it becoming inadmissible in a court of law.

The Genetics Department has a good working relationship with the Forensic Science Laboratory and Free State Crime Scene Management of the Division Forensic Services of the SAPS. The mock crime scene was set up and assessed in collaboration with the Crime Scene Management Division of the SAPS. Although the SAPS provides specialist advanced training to its staff members, the university hopes to improve employability for students through such programmes.

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