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11 July 2022 | Story Andre Damons | Photo Supplied
Prof Martie Smith and Prof Drik Opperman
Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.

Flavours and fragrances have a wide application in the food, feed, cosmetic, chemical and pharmaceutical sectors. Many flavour compounds are still produced via chemical synthesis or via extraction from plant or animal sources. However, there is increasing interest in their bio-production or the use of flavour compounds of (micro) biological origin. 

One reason for this shift is that chemical synthesis often uses environmentally unfriendly processes. Chemical synthesis usually also produces racemic mixtures with the second enantiomer, mirror image of the looked-for compound, often having undesirable organoleptic properties. Furthermore, the consumer has developed a “chemophobia”-attitude towards synthetic chemical compounds, especially when related to food and home-care products.  This applies even to nature-identical compounds – products that occur in nature but are produced via a non-natural chemical process. Products produced with the use of enzymes or microbes from “natural” substrates can be labelled “natural”. The flavour and fragrance industry thus pay higher prices for such products labelled as “natural”.  

The invention

A University of the Free State (UFS) team, led by Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry are conducting exciting research in this area. They filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.  

The invention relates to a process for the enzymatic in-chain hydroxylation of C12 to C16 fatty acids, alcohols, and alkanes. Hydroxylation of C12 fatty acid and alcohol provides routes for the synthesis of “natural” δ-dodecalactone. The advantage of these routes is that they do not rely on massoia lactones. Massoia lactones are derived from the bark of Massoia trees which grow in Indonesia. Harvesting of the bark kills the trees.  

The cytochrome P450 enzymes (P450s) claimed in this patent are to the inventors’ knowledge the most regioselective enzymes described thus far that can be used for the synthesis of δ-dodecalactone from lauric acid or 1-dodecanol. The approach that the technology takes is to claim cytochrome P450 enzymes that share 70 % amino acid identity to a set of selected P450s for the regioselective hydroxylation of lauric acid and 1-dodecanol to synthesise δ-dodecalactone.

Still in early stage

The current state of development is early stage with the technology only demonstrated in the laboratory on a small scale (100-200 ml). Before the technology can be commercialised the team would need to further improve the regioselectivity and stability of the P450s and proof that the reactions can be scaled up in bioreactors. The technology will probably be delivered as an enzyme (amino acid sequence) with the desired properties. 

There are other research groups working on a synthetic biology approach for the de novo synthesis of δ-dodecalactone from glucose by genetically engineered microbes. It is still unclear how such a process will compare in terms of product yields, economics and environmental impact with the processes proposed by the UFS patent.

If the team had to partner with a commercial company, their first choice would be to work with an established flavour and fragrance company. Another possibility would be the small French flavour and fragrance company that Dr Alizé Pennec, the post-doc and co-inventor who initially discovered the unique P450 activity, is working for.

Please view the videos for more information on patents.

The Vice-Rector: Research and Internationalisation has released two new calls for applications for funding. Academic staff and researchers are encouraged to submit applications for these funds. At this stage we are not accepting projects from Research Fellows. 

The two funds are: 

1.  The Industrial Engagement Fund 
2.  The Intellectual Property Commercialisation Fund

Each fund has its own guidelines and application process. The guidelines are attached. The applications must be filled in on RIMS.

The RIMS application forms can be found through this link

For more information please click the documents below:



News Archive

From music to theology: Stats Unit valuable in research process
2017-02-23

Description: Prof Robert Schall Tags: Prof Robert Schall

Prof Schall, head of the UFS Statistical Consultation Unit
Photo: Leonie Bolleurs

Whether it is analysing data on church attendance, climate change in the Northern Cape or injuries among elite female hockey players, the Statistical Consultation Unit at the University of the Free State (UFS) can assist researchers from the planning of research to publication therof.

Many students and researchers think that the time to consult a statistician is after their research data has been collected. According to Prof Robert Schall, head of the unit, the most significant contribution a statistician can make to a research project is often during its planning. Preferably all researchers should consult the unit early in the research process.

Statistical consultation service free for postgraduates

The consultation unit, established in 2014 in the Department of Mathematical Statistics and Actuarial Science, provides support to all UFS researchers. This service is rendered to postgraduate students at no charge.

“The unit can make a contribution throughout the research process, from the planning of the research project, through the analysis of research data, up to the publication of the findings. I have been involved in projects where, for example, a few very simple changes to the design of a questionnaire would have saved the researcher and the statistician a lot of trouble. It will be beneficial for researchers to have their questionnaires and study proposals (where relevant), reviewed by a statistician,” Prof Schall said.

“The unit can make a contribution
throughout the research process,
from the planning of the research
project, through the analysis of
research data, up to the publication
of the findings.”

Fascinating research topics deliver fascinating data
The professor assisted in a study for the Department of Soil, Crop and Climate Sciences to determine whether rainfall in the Northern Cape had changed over the past 90 years, potentially indicating climate change.

Other interesting projects he has worked on came from the Department of Exercise and Sport Sciences. “Who will not be fascinated by data sets on aspects of rugby, cricket or even netball? One significant finding was a predictor of injury in elite female hockey players. The PhD student identified a pre-season test which predicted the occurrence of an in-season injury with 100% specificity and 100% sensitivity. The finding was quite surprising, and, if the results can be replicated, obviously would be useful in the prevention of injuries,” he said.

This is, of course, not an exhaustive list of projects the unit has worked on. “Not in my wildest dreams would I have expected to be involved in projects coming from the Faculty of Theology, or from the Odeion School of Music,” Prof Schall said.

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