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11 July 2022 | Story Andre Damons | Photo Supplied
Prof Martie Smith and Prof Drik Opperman
Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.

Flavours and fragrances have a wide application in the food, feed, cosmetic, chemical and pharmaceutical sectors. Many flavour compounds are still produced via chemical synthesis or via extraction from plant or animal sources. However, there is increasing interest in their bio-production or the use of flavour compounds of (micro) biological origin. 

One reason for this shift is that chemical synthesis often uses environmentally unfriendly processes. Chemical synthesis usually also produces racemic mixtures with the second enantiomer, mirror image of the looked-for compound, often having undesirable organoleptic properties. Furthermore, the consumer has developed a “chemophobia”-attitude towards synthetic chemical compounds, especially when related to food and home-care products.  This applies even to nature-identical compounds – products that occur in nature but are produced via a non-natural chemical process. Products produced with the use of enzymes or microbes from “natural” substrates can be labelled “natural”. The flavour and fragrance industry thus pay higher prices for such products labelled as “natural”.  

The invention

A University of the Free State (UFS) team, led by Prof Martie Smit and Prof Dirk Opperman in the Department of Microbiology and Biochemistry are conducting exciting research in this area. They filed a patent entitled “Process for the chemical modification of alkanes, fatty acids and fatty alcohols”.  

The invention relates to a process for the enzymatic in-chain hydroxylation of C12 to C16 fatty acids, alcohols, and alkanes. Hydroxylation of C12 fatty acid and alcohol provides routes for the synthesis of “natural” δ-dodecalactone. The advantage of these routes is that they do not rely on massoia lactones. Massoia lactones are derived from the bark of Massoia trees which grow in Indonesia. Harvesting of the bark kills the trees.  

The cytochrome P450 enzymes (P450s) claimed in this patent are to the inventors’ knowledge the most regioselective enzymes described thus far that can be used for the synthesis of δ-dodecalactone from lauric acid or 1-dodecanol. The approach that the technology takes is to claim cytochrome P450 enzymes that share 70 % amino acid identity to a set of selected P450s for the regioselective hydroxylation of lauric acid and 1-dodecanol to synthesise δ-dodecalactone.

Still in early stage

The current state of development is early stage with the technology only demonstrated in the laboratory on a small scale (100-200 ml). Before the technology can be commercialised the team would need to further improve the regioselectivity and stability of the P450s and proof that the reactions can be scaled up in bioreactors. The technology will probably be delivered as an enzyme (amino acid sequence) with the desired properties. 

There are other research groups working on a synthetic biology approach for the de novo synthesis of δ-dodecalactone from glucose by genetically engineered microbes. It is still unclear how such a process will compare in terms of product yields, economics and environmental impact with the processes proposed by the UFS patent.

If the team had to partner with a commercial company, their first choice would be to work with an established flavour and fragrance company. Another possibility would be the small French flavour and fragrance company that Dr Alizé Pennec, the post-doc and co-inventor who initially discovered the unique P450 activity, is working for.

Please view the videos for more information on patents.

The Vice-Rector: Research and Internationalisation has released two new calls for applications for funding. Academic staff and researchers are encouraged to submit applications for these funds. At this stage we are not accepting projects from Research Fellows. 

The two funds are: 

1.  The Industrial Engagement Fund 
2.  The Intellectual Property Commercialisation Fund

Each fund has its own guidelines and application process. The guidelines are attached. The applications must be filled in on RIMS.

The RIMS application forms can be found through this link

For more information please click the documents below:



News Archive

Researcher uses NRF funding for studies to conserve plant and animal life
2017-04-18

Description: Butterfly Tags: Butterfly

It is difficult to survey all different types of
plants and animals and is therefore necessary to
choose one representative group. Butterflies are
relatively cheap and easy to sample. They are
known to be linked to specific habitats and to
respond to human pressures, such as farming.
Photo: Dr Falko Buschke


Earth is the only planet we know of that contains life. The variety of different plants and animals is remarkable: from the giant whales that swim our oceans, to the tiny mosses that grow on the shaded sides of rocks.  Many of these plants and animals are important to humans. For example, trees provide us with oxygen to breathe, bees pollinate our crops and owls control pests. More importantly though, we can tell a lot about society from the way it cares for nature. Humans are the custodians of the planet and the way we care for nature reflects the way we value life.

Dr Falko Buschke, Lecturer at the Centre for Environmental Management at the University of the Free State, is interested in understanding how the distribution of biodiversity [the variety of living things in nature] in time and space influences the way we should conserve and manage nature.

Earth is losing biodiversity faster than at any time in human history

The planet is losing biodiversity faster than at any time in human history. “There is an urgency to conserve plants and animals before they are lost forever. Nature is complex, so the way we study it should embrace this complexity. We should not rely on limited data on one type of species from one place and assume that it will also apply elsewhere. Instead, it is important that biodiversity research is comprehensive in the types of plants and animals while also considering that ecological and evolutionary processes vary through time and across geographic space,” he said.

To conduct his research, Dr Buschke uses a variety of research tools, including biological data surveyed directly from nature, spatial data from satellite remote sensing and geographic information systems databases, and data generated though custom-built computer simulations.

"There is an urgency to conserve
plants and animals before they
are lost forever."

Field work in the eastern Free State
Although parts of the eastern Free State are considered a global priority for biodiversity conservation, it is mainly privately owned commercial farmland. This means that it is important that plants and animals can survive despite living side by side with agricultural production.

“My project investigates whether the sandstone outcrops, known as inselbergs (island-mountains), are safe havens for plants and animals. Because it is difficult to survey all the different types of plants and animals, it is necessary to choose one representative group. That is where butterflies come in. Butterflies are relatively cheap and easy to sample. They are known to be linked to specific habitats and to respond to human pressures, such as farming,” he said. “Once this butterfly data is collected, it can be linked to satellite information on plant growth patterns. This will provide a clearer picture of whether plants and animals can persist side-by-side with commercial agriculture”.

Dr Buschke has just begun surveys that will carry on until the end of this year. “This 12-month project is funded under the Foundational Biodiversity Information Programme through the South African National Biodiversity Institute (SANBI) and the National Research Foundation (NRF).

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