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10 May 2022 | Story Anthony Mthembu | Photo Supplied
Alina Ntsiapane
Alina Ntsiapane obtained second place in the partners division of the ILRI CapDev Grand Challenge research pitching contest.

Alina Ntsiapane, a PhD student at the University of the Free State, obtained second place in the partners category of the International Livestock Research Institute’s (ILRI) CapDev Grand Challenge research pitching contest, which took place on 13 April 2022. The pitching contest is the first part of the CapDev Grand Challenge, which is a 10-month process aimed at equipping scientists with the necessary skills to contribute to new research. 

Presenting Research to a Tough Panel of Judges 

Ntsiapane was one of 30 contestants who presented their research virtually to a panel of esteemed judges. “It was not easy, it was very challenging for me because it was my first time presenting my PhD study and I had to do it live on an international platform,” expressed Ntsiapane. Although each contestant is thoroughly prepared for their respective presentations, Ntsiapane argues that some of the questions asked by the judges can be quite daunting. “Some of their questions were very challenging and I did not know how to respond to them, but they made me aware of ways in which I needed to improve my research,” she stated. However, regardless of the intensity of the pitching contest, Ntsiapane’s research allowed her to progress to the next stage of the CapDev Grand Challenge. She will be part of the rigorous 10-month training process that will begin in June 2022.

Ntsiapane’s Research Project

Ntsiapane’s PhD research focuses on the production of smallholder wool as a means to improve livelihoods in both Thaba ’Nchu and Botshabelo in the Free State. In fact, in the research Ntsiapane highlights that there has been a significant decline in the production of wool within the last three decades. As such, Ntsiapane believes it is imperative to create spaces that allow for the training of small-scale farmers, so that the production of wool can still be a possibility.
Consequently, Ntsiapane hopes that the 10-month training she will receive from the CapDev Grand Challenge will not only allow her to grow but will assist in opening doors for her. “I’m hoping to get exposure and to make connections with policy makers and the donors as well. This will assist me in achieving my goals,” she explained. 

Future Endeavours After the Training Course

Subsequent to the training course, Ntsiapane would like to utilise that knowledge by continuing to make her most recent project a reality. Ntsiapane is currently working on developing a television show aimed at providing adequate training to small-scale farmers, so that they are equipped with the necessary knowledge and understanding of the industry in which they find themselves. As such, being part of the CapDev Grand Challenge will allow her to learn some of the necessary ways in which this dream could become a reality. 

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Dr Charlotte Boucher and Lindi Heyns examine possible anti-microbial activity in the skin of Western olive toad species
2014-12-22

 

Researchers Lindi Heyns and Dr Charlotte Boucher are working together on an interdisciplinary project between the Departments of Zoology and Entomology and Veterinary Biotechnology at the University of the Free State (UFS). The focus of their research is on the preliminary biochemical description of skin secretions in some South African toads.

The project forms part of an Honours study executed by Dwayne Pike under Heyns’ supervision. He is co-supervised by Dr Boucher who is assisting with the biochemical and microbiological assays.

Dr Boucher said, “Amphibians are characterised by the presence of cutaneous glands spread over the skin. There are two types of glands, namely mucous and granular (poison), located on the inner surface of the epidermis. Mucous glands are widely dispersed over the skin, while granular glands can be grouped and enlarged in specific regions. Mucous glands are generally associated with maintenance of humidity and cutaneous respiration, whereas granular glands function in chemical defence against predators and/or microbial infection. Studies indicate that the compounds produced by the granular glands belong to numerous chemical classes with diverse pharmacological activities.”

The products secreted by granular glands are rich in low molecular weight constituents of varied molecular types, including proteins, peptides and toxins. These secretions make the toad foul-tasting to predators and even toxic to other frog species. In addition, amphibians offer an attractive source of novel antimicrobials. Studies indicate that as a response to inhabiting microorganism-rich environments they synthesise and secrete a diverse array of antimicrobial peptides (AMPs) as an innate form of defence. Extensive research by various other research groups has been carried out on antimicrobial peptides of the genus Rana; however, hardly any studies have investigated the antimicrobial activity of African frog species.

The focus of this preliminary project is to determine the protein composition of the glandular secretions of the Western olive toad (Amietophrynus poweri), using biochemical tests, such as SDS-PAGE also known as protein gel electrophoresis combined with mass-spectrometry used to identify unknown peptides and proteins. This will give us an overview of the composition of the glandular secretions. Furthermore, we are also looking at microbiological tests, which include assays that test for possible anti-microbial activity against various bacterial and fungal species.

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