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08 December 2023 | Story Leonie Bolleurs | Photo Charl Devenish
Thabo Dithebe
When Thabo Dithebe walked across the stage in the Callie Human Centre, a childhood dream came true, despite several difficulties he had to deal with during his studies.

Thabo Dithebe received his Bachelor of Management Leadership (BML) qualification during the University of the Free State’s December graduation ceremonies. His life journey of resilience, adaptability, and a willingness to embrace unexpected opportunities is an inspiring story that needs to be told. 

Born in the farming community of Bothaville, Thabo initially wanted to pursue a career in engineering, and he chose maths, science, and technical subjects in high school. However, a temporary job in retail near home and experiencing the toll of retrenchment redirected his path to sales and marketing and pushed him to apply for a BML in the UFS Business School.

“When I enrolled for the BML, my childhood dream of going to university became a reality. Although the journey was not easy, it was worth pursuing,” says Thabo, who is employed as an area sales manager at Marltons Pet Care.

Balancing studies and responsibilities at home

In addition to managing the pressure of being stretched to the limit in class, Thabo also had to balance his studies with the responsibilities at home – taking care of his sick and disabled daughter.

“Balancing these commitments was always a challenge, but I was fortunate to have a supportive caregiver who understood the needs of children living with disabilities. There were tough times, especially during submission seasons when my daughter would fall ill. In those moments, I had to prioritise her health, temporarily setting aside my studies until she received proper care at the hospital,” he states.

Besides caring for his daughter and being retrenched, he also went through a breakup. Thabo believes that things could have turned out differently if he had stopped imagining and dreaming, because he had every reason to give up. He describes reaching this significant milestone in his life as ‘unreal’. 

He says that he was motivated to persevere, because from the first day he enrolled, he wanted to make his family proud of him. “There were times when I asked myself if I had what it takes to complete the programme. Should I choose not to complete the course, what would I say to my son when life knocks him down, when he sees me giving up? How do I face my mother if I came back defeated? I had good people on my side who wanted me to succeed,” he says.

Ready to take on the next challenge

Having reached this significant milestone in his life, Thabo is ready for the next challenge. “I am hoping to be accepted into the PGDip in Business Administration programme for the 2024 intake,” he says. 

He advises others who face challenges while pursuing their educational goals to understand that their situation is not permanent and that there is strength in adversity. “It is important to celebrate the small victories often and to associate yourself with people who work harder than you,” he adds.

On 7 December 2023, as Thabo walked across the stage, he looked for the face of his mother in the audience – a lady who made several sacrifices to raise him and his five siblings. “I hope that when she saw me, she was very proud of me,” he concludes. 

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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