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22 February 2023 | Story Leonie Bolleurs | Photo Supplied
Rodney Moffett
Prof Rodney Moffett was presented with the Silver Medal Award. At the ceremony were, from the left: Prof Glynis Goodman-Cron, SAAB President, Prof Rodney Moffett, and Prof Sandy-Lynn Steenhuisen.

Prof Rodney Moffett, Honorary Research Fellow in the Department of Plant Sciences at the University of the Free State (UFS) and Top Research Fellow in the Afromontane Research Unit (2018-2019) on the UFS Qwaqwa Campus, was recently awarded a Silver Medal from the South African Association of Botanists (SAAB) at their 48th annual conference that took place in Polokwane.

Prof Sandy-Lynn Steenhuisen, Subject Head and Associate Professor in the Department of Plant Sciences on the UFS Qwaqwa Campus, says, “It was an absolute joy and honour to be able to nominate and present a SAAB Silver Medal to Prof Moffett, who is an iconic figure and friend in our department. I was so happy that he could receive his award in person, with his daughter visiting from the United Kingdom. He inspires my research group so much with his knowledge of plants of the Eastern Free State and through the wealth of specimens he’s contributed to the QWA herbarium.”

The conference with the theme, ‘Plants, Health and Prosperity’, was hosted by the University of Limpopo’s Faculty of Science and Agriculture under the School of Molecular and Life Sciences in the Department of Biodiversity.

Outstanding research contributions 

According to Prof Steenhuisen, Prof Moffett received the Silver Medal Award for his outstanding research that contributed to the advancement of botany and plant sciences in South Africa.

Since his retirement, Prof Moffett has published a number of book chapters, books, reports, and articles on various aspects of fauna and flora. 

He sole-authored eight books and authored/co-authored more than 36 peer-reviewed articles in scientific journals covering mainly the taxonomy of plant genera such as Sarcocaulon, Rhus, and Searsia, and checklists of vascular plants and their medicinal uses for the Qwaqwa and Lesotho regions. 

Around 2006, Prof Moffett pursued his passion to document and describe the ethnobotanical uses of plants used by the Basotho people of Lesotho and South Africa. His books focused, for instance, on the natural history and Sesotho names of the plants used by the Basotho of the Eastern Free State and their ethnobotanical uses, the taxonomic works on the grasses of the Eastern Free State (still the most detailed grass identification guide for the area to date), and the flora and fauna of the Clarens Village Conservancy (a field guide). 

Prof Moffett has also published a scientific bibliography of the Drakensberg, Malotis and adjacent lowlands, which Prof Steenhuisen describes as “a mammoth task and one which he is continually updating and expanding, with another volume in preparation”.

Prof Steenhuisen, who nominated Prof Moffett for the Silver Medal Award, says that as a highly respected naturalist in the Qwaqwa region, he is continually consulted by conservation authorities, researchers, and postgraduate students on the flora of the area. He also regularly presents his research at garden and botanical societies. 

Among the books he has published are Sesotho names of plants and animals and plants used by the Basotho (2010), A biographical dictionary of contributors to the natural history of the Free State and Lesotho (2014), and Meriana ya dimela tsa Basotho – Basotho medicinal plants (2016 and 2020). In 2020, he also published A Scientific Bibliography of the Drakensberg, Maloti and adjacent Lowlands.

Prof Steenhuisen states that Prof Moffett’s books are treasured resources for anyone working in the Maloti-Drakensberg area. 

Best poster presentation

Joining researchers, postgraduate students, and experts from other universities and organisations at the conference, was the Plant Ecology Research Group of Prof Steenhuisen, her postgraduate students, and a postdoctoral fellow involved in invasive species and climate change research. Postgraduates from three other research groups (Plant Biotechnology, Proteomics, and Ethnobotany supervised by Dr Arun Gokul, Dr Rudo Ngara, and Prof Anofi Ashafa) on the Qwaqwa Campus also attended the conference. 

Dr Makoena Moloi, Senior Lecturer in the Department of Plant Sciences on the Bloemfontein Campus, and her postgraduate students researching aspects of plant ecophysiology, were also present at the conference, together with master’s candidate Orateng Sedimo, who is supervised by Dr Lize Joubert, Senior Lecturer in the department. Sedimo presented his honours research and won a prize for presenting the best Honours poster in the category Molecular Systematics. 

Besides being well-represented at the 48th SAAB annual conference and being awarded for its outstanding contributions to botany and plant sciences, academics from the UFS had the opportunity to learn from and network with some of the best scientists in the field. This included A1-rated scientist, Prof Steven Johnson from the University of KwaZulu-Natal, (Prof Steenhuisen’s supervisor for her PhD), and B2-rated Prof Anton Pauw from Stellenbosch University. Prof Johnson and Prof Pauw were respectively awarded a Gold and second Silver Medal from SAAB. 

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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