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07 June 2023 | Story André Damons | Photo Supplied
Dr Michael Pienaar and Dr Edward C Netherlands
Dr Michael Pienaar, Senior Lecturer and specialist in the UFS Department of Paediatrics and Child Health, is a finalist in two categories of this year’s NSTF-South32 Awards. Dr Edward C Netherlands, Senior Lecturer in the UFS Department of Zoology and Entomology, is also in the running for his first NSTF-South32 Award.

Dr Michael Pienaar, Senior Lecturer and Specialist in the University of the Free State (UFS) Department of Paediatrics and Child Health, is a finalist in two categories of the 2023 NSTF-South32 Awards, popularly known as the ‘Science Oscars’ of South Africa.

Dr Pienaar, who has been working in paediatric critical care since 2019 and sees the care of critically ill children as his mission and calling in life, has been named a finalist in the TW Kambule-NSTF Award: Emerging Researcher and the NSTF-SAMRC Clinician-Scientist Award categories.

Another UFS staff member, Dr Edward C Netherlands, Senior Lecturer in the Department of Zoology and Entomology, is also a finalist in the TW Kambule-NSTF Award: Emerging Researcher category.

The NSTF-SAMRC Clinician-Scientist Award, sponsored by the South African Medical Research Council (SAMRC), is a new award for an outstanding contribution by a clinician-scientist, with a focus on work to enhance life and improve community health. This new category is modelled on the Emerging Researcher category.

‘Validating to have research recognised’

Dr Pienaar says it is humbling to be considered for these awards alongside formidable, accomplished, and innovative clinicians and scientists. “This is all somewhat surreal, as I was not anticipating being shortlisted as a finalist. It is validating to have had my research, which I am committed to, recognised on this platform. I am very grateful to the School of Clinical Medicine and the Faculty of Health Sciences and University of the Free State for nominating me for these awards.”

He was nominated by Dr Claire Armour Barrett, Clinical Research Director and Specialist Physician in the UFS School of Clinical Medicine.

Dr Pienaar says his current research focusses on applied machine learning in paediatric critical care. He has been working on the development of machine learning models that can be used in clinical practice to improve patient care.

“The work leading to these nominations focused on triage and identification of critically ill children in South Africa. Currently, I am working towards machine learning models implemented in point-of-care ultrasonography and mechanical ventilation.

“I am pleased just to be shortlisted for these awards. I think this provides important visibility for my work, which I feel has major long-term implications for clinical practice. I am also very happy to represent the research work being done in the Faculty of Health Sciences and the UFS on this platform.”

On what it will mean to win a ‘Science Oscar’, Dr Pienaar says, “While this would advance my career, I am very aware that all the candidates would be worthy winners. As it is, the opportunity to be a scholar is reward unto itself, and I am very privileged to be able to do the research I do. As ever, I am very grateful to the University and the Faculty of Health Sciences as well as the National Research Foundation for supporting my work.”

‘Honour and privilege’

Dr Netherlands, who was nominated by Prof Corli Witthuhn, former UFS Vice-Rector for Research and Internationalisation, says, “It is a great honour and privilege to represent the UFS.”

His work as a parasitologist and herpetologist involves specialised research in the study of blood parasites. His current research focuses on the diversity, evolution, and ecological implications of blood parasites in herpetofauna (reptiles and amphibians of a particular region).

“Being shortlisted as a finalist for this award will be a humbling achievement in my career, that may lead to new opportunities and new collaborations,” Dr Netherlands says. “Furthermore, being shortlisted provides a sense of validation and affirmation for the effort that has gone into conducting my research.”

The announcement of the winners will take place at the NSTF-South32 Awards Gala Event on 13 July 2023.

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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