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09 February 2024 | Story EDZANI NEPHALELA | Photo SUPPLIED
Jerry Dlamini
Dr Jerry Dlamini, lecturer and researcher specialising in agronomy within the Department of Soil, Crop, and Climate Sciences at the University of the Free State (UFS), is at the forefront of pioneering research in this field.

Greenhouse gas emissions represent a significant global concern, driving climate change on a massive scale. This concern is particularly pronounced in rainfed agriculture, where understanding and addressing these emissions are crucial for ensuring sustainable agricultural practices. 

In South Africa, rainfed agriculture is vital in food production, contributing substantially to the nation's agricultural output. However, this sector also stands as a notable contributor to greenhouse gas emissions, primarily through activities such as livestock farming, fertiliser use, and changes in land use.

Dr Jerry Dlamini, a distinguished lecturer and researcher specialising in agronomy within the Department of Soil, Crop, and Climate Sciences at the University of the Free State (UFS), is leading pioneering research in this field. His current project, @CROPGas on X, funded by the European Joint Programme (EPJ), with a budget of R22 million, focuses on investigating the impact of various conservation agriculture interventions on greenhouse gas emissions, primarily targeting nitrous oxide (N2O), methane (CH4), and carbon dioxide (CO2).

This two-year project, which commenced in December 2022 and concludes in December 2024, is a collaborative effort between European and African universities and institutions, including Rothamsted Research (UK), University College Dublin (Ireland), University of Nottingham (UK), University of Poznan (Poland), British Geological Surveys (BGS), University of Zambia (Zambia), University of Zimbabwe, and Lilongwe University of Agriculture and Natural Resources (Malawi). 

Dr Dlamini’s preliminary findings from the UFS Kenilworth Experimental Farm indicate that climate-smart agriculture interventions, such as legume rotation and no-till practices, have the potential to reduce the intensity of greenhouse gas emissions, particularly highly radiative gases like N2O.

“This is a significant finding,” Dr Dlamini noted, “as N2O has a global warming potential 100 times greater than CO2 over a 100-year horizon, meaning its impact on ozone depletion persists far longer despite being emitted in smaller quantities.”

Looking ahead, Dr Dlamini advocates for increased research efforts to quantify greenhouse gas emissions from South African croplands. He emphasises the importance of field-based measurements, akin to methodologies employed by other nations, to enhance the accuracy and effectiveness of South Africa's greenhouse gas inventories submitted annually to the United Nations Framework Convention on Climate Change (UNFCCC) and to devise effective mitigation strategies. 

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Newly operational sequencing unit in genomics at UFS
2016-09-09

Description: Next Generation Sequencing  Tags: Next Generation Sequencing

Dr Martin Nyaga and his research assistant,
Tshidiso Mogotsi in the Next Generation
Sequencing Laboratory.
Photo: Charl Devenish

The Next Generation Sequencing (NGS) unit at the UFS was established as an interdisciplinary facility under the Directorate for Research Development, Faculty of Health Sciences and Faculty of Natural and Agricultural Sciences.

The aim of the NGS facility is to aid internal and external investigators undertaking studies on Deoxyribonucleic acid (DNA) sequencing, assembly and bioinformatics approaches using the more advanced Illumina MiSeq NGS platform.

The NGS unit became operational in 2016 and is managed by Dr Martin Nyaga and administered through the office of the Dean, Faculty of Health Sciences, under the leadership of Prof Gert Van Zyl. Dr Nyaga has vast experience in microbial genomics, having done his PhD in Molecular Virology.

He has worked and collaborated with globally recognised centres of excellence in Prokaryotic and Eukaryotic genomics, namely the J. Craig Venter Institute and the Laboratory of Viral Metagenomics, Rega Institute, among others.

The unit has undertaken several projects and successfully generated data on bacterial, viral and human genomes. Currently, work is ongoing on bacterial and fungal metagenomics studies through 16S rRNA sequencing.

In addition, the unit is also working on plasmid/insert sequencing and whole genome sequencing of animal and human rotaviruses. The unit has capacity to undertake other kinds of panels like the HLA, Pan-cancer and Tumor 15 sequencing, among others.

Several investigators from the UFS including but not limited to Prof Felicity Burt, Prof Wijnand Swart, Dr Frans O’Neil, Dr Trudi O'Neill, Dr Charlotte Boucher, Dr Marieka Gryzenhout and Dr Kamaldeen Baba are actively in collaboration with the NGS unit.

The unit has also invested in other specialised equipment such as the M220 Focused-ultrasonicator (Covaris), 2100 Bioanalyzer system (Agilent) and the real-time PCR cycler, the Rotor-Gene Q (Qiagen), which both the UFS and external investigators can use for their research.

Investigators working on molecular and related studies are encouraged to engage with Dr Nyaga on how they would like to approach their genomics projects at the UFS NGS unit. 

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