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SADoCoL
Betsie Human and Elandré Williams, analysts at the South African Doping Control Laboratory (SADoCoL) at the University of the Free State (UFS), will be involved in sample preparation, analysis and data processing at the upcoming Olympic and Paralympic Games in Paris, France.

Two staff members from the South African Doping Control Laboratory (SADoCoL) which is housed at the University of the Free State (UFS), have been selected to work at the upcoming Olympic and Paralympic Games, in Paris, France.

Elandré Williams and Betsie Human will support the Paris laboratory during both games. The Olympic Games will take place from 26 July to 11 August 2024 and the Paralympic Games from 28 August to 8 September 2024.

Williams will be involved in steroid profile analysis, which includes sample preparation, analysis and data processing by Gas Chromatography (GC) and Isotope Ratio Mass Spectrometry (GC-C-IRMS).

Part of the fight against doping 

“I am excited, optimistic and privileged to have been given this opportunity, but I have to say that I am also quite nervous as this is most probably the biggest sporting event of the year. Being a part of the fight against doping in sport remains a great responsibility as what we do directly impacts the athletes,” says Williams.

She says is looking forward to the entire experience, from doing what she loves on an international level, meeting other analysts in the field and being part of the fight against doping in sport on an Olympic level.

This is her first big international sporting event.

“I am also looking forward to learning from other experts in the field who have more experience and to witness the procedures and the manner in which the laboratory operates at this time where the sample numbers are extremely high with the added pressure to finalise results in short turn-around times. This is a great opportunity for growth, both individually and in my field of expertise, in the scientific and the doping control field.

“It will definitely be an advantage for me as an analyst to get exposure to how the entire analytical procedure is executed in another laboratory, as well as insight into possible new techniques and advancements that I will be able to apply back at SADoCoL. I also think this is a great way to improve my ideas, perspectives and level of expertise as I will be working and witnessing other scientific experts in the doping control field.”

Managing workflow and logistics at the Games

Human, who was an analyst at the 2010 Soccer World Cup in South Africa, says she is both nervous and excited for this experience. 

“I was a junior analyst at SADoCoL during the 2010 Soccer World Cup, but you cannot compare a single-sport discipline with a multisport discipline like the Olympic Games – The Games will be exponentially bigger.

“In the past 14 years doping control as a whole has grown significantly. New technologies, updated requirements, more sensitive testing methods have emerged – this will be a new experience,” says Human.

She will also be involved with sample preparation/analysis/data processing and says she is looking forward to seeing how the work-flow and logistics associated with the Games (massive amounts of samples/tight deadlines etc) is managed in a high through-put laboratory.

“I am of course also looking forward to meeting analysts from other labs – we are a bit secluded here at the southern tip of Africa. Collaboration between labs is tricky when your closest neighbour is in Europe.

“It is always eye-opening to see how other labs manage similar situations (even though an Olympics is quite different from normal routine days) – exposure to new techniques and alternative thinking has a way of elevating your own thought processes and it promotes growth – both as an individual and as a doping control analyst.”

Immensely proud

Hanno du Preez, Director of SADoCoL, says the laboratory personnel are immensely proud that two of their staff members were chosen to participate in this international event, which for many scientists is the peak of their career. Similarly, this provides acknowledgement to the staff members for the area in which they have been working.

“It is only a select few who are requested to provide service at the Olympic Games. The work conducted in an Olympic laboratory provides experience which cannot be gained elsewhere. The workload and fast-paced analysis is something which the personnel are used to, but the Olympics will bring a different dimension to the processes. 

“We are excited to see what Betsie and Elandré bring back, with regards to new viewpoints on processes which are similar in all anti-doping laboratories. Individual experiences uplift everyone in a regulated business unit such as SADoCoL and also ensures improved relationships between laboratories, as other anti-doping laboratories will be represented at the Games as well. We wish them all the best for the experience, and we thank them for being dedicated ambassadors for SADoCoL and the UFS.”

News Archive

UFS study on cell development in top international science journal
2008-09-16

A study from the University of the Free State (UFS) on how the change in the packaging of DNA with cell development influenced the expression of genes, will be published in this week’s early edition of the prestigious international, peer-reviewed science journal, the Proceeding of the National Academy of Sciences of the USA (PNAS).

The PNAS journal has an impact factor of 10, which means that studies published in the journal are, on average, referred to by ten other scientific studies in a two year period. The South African Journal of Science, by comparison, has an impact factor of 0.7.

The UFS study, funded by the Wellcome Trust and the National Research Foundation (NRF), looked at how the change in the packaging of DNA with cell development influenced the expression of genes. It is very relevant to research on stem cells, an area of medicine that studies the possible use of undifferentiated cells to replace damaged tissue.

Prof. Hugh Patterton, of the Department of Microbial, Biochemical and Food Biotechnology at the UFS, who led the study, said: "We are extremely proud of this study. It was conceived in South Africa, it was performed in South Africa, the data were analysed in South Africa, and it was published from South Africa."

When a gene is expressed, the information encoded in the gene is used to manufacture a specific protein. In eukaryotes, which include humans, there is approximately 1m of DNA, containing the genes, in every cell. This length of DNA has to fit into a cell nucleus with a diameter of only about 10 micrometer. In order to fit the DNA into such a small volume, eukaryotic cells wrap their DNA onto successive protein balls, termed nucleosomes. Strings of nucleosomes, resembling a bead of pearls, is folded into a helix to form a chromatin fiber. The study from the UFS investigated how the binding of a specific protein, termed a linker histone, that binds to the length of DNA between nucleosomes, influenced the formation of the chromatin fiber and also the activity of genes.

"We found that the linker histone bound to chromatin in yeast, which we use as a model eukaryote, under conditions where virtually all the genes in the organism were inactive. It was widely believed that the binding of the linker histone caused the inactivation of genes. We studied the relationship between the amount of linker histone bound in the vicinity of each gene and the expression of that gene for all the genes in yeast, using genomic techniques. We made the surprising discovery that even through the linker histone preferentially bound to genes under conditions where the genes were shut off, this inactivation of genes was not caused by the binding of the linker histone and folding of the chromatin,” said Prof. Patterton.

He said: “Instead our data strongly suggested that the observed anti-correlation was due to the movement of enzymes along the DNA molecule, involved in processing the information in genes for the eventual manufacture of proteins. This movement of enzymes displaced the linker histones from the DNA. This finding now requires a rethink on aspects of how packaging of DNA influences gene activity."

Prof. Patterton said that his research group, using the Facility for Genomics and Proteomics as well as the Bioinformatics Node at the UFS, was currently busy with follow-up studies to understand how other proteins in nucleosomes affected the activities of genes, as well as with projects to understand how chemicals found in red wine and in green tea extended lifespan. "We are certainly having a marvelous time trying to understand the fundamental mechanisms of life, and the UFS is an exciting place to be if one was interested in studying life at the level of molecules," he said.


Media Release
Issued by: Lacea Loader
Assistant Director: Media Liaison
Tel: 051 401 2584
Cell: 083 645 2454
E-mail: loaderl.stg@ufs.ac.za  
18 September 2008
 

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