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Proteins and enzymes the focus of Prof Dirk Opperman’s inaugural lecture

27 May 2024 | Story Leonie Bolleurs | Photo Supplied

Prof Dirk Opperman, a distinguished biochemist in the Department of Microbiology and Biochemistry, recently (21 May 2024) delivered his inaugural lecture on the Bloemfontein Campus of the University of the Free State (UFS).

The title of his lecture was: Exploring, Exploiting, and Evolving Life at the Atomic Level.

Prof Vasu Reddy, Deputy Vice-Chancellor: Research and Internationalisation at the UFS, welcomed guests, stating, "An inaugural lecture is a major milestone, celebrating a life’s work that culminates in the title of professor. It marks an important chapter in an academic career, with much more to be achieved in the journey of producing important knowledge.”

He believes that an event such as this highlights the university’s pride in the achievements of its academic staff and aligns with Vision 130. “The UFS is proud to host such lectures, as they are significant moments to reveal and showcase the value of excellence in our knowledge pool in research, teaching, and innovation. As a university, we strive to make a difference through groundbreaking work, particularly in addressing society's challenges,” said Prof Reddy, emphasising that this topic truly speaks to the university’s commitment to impactful work in the hard sciences.

Deciphering the unknown

The topic of the lecture captures the essence of Prof Opperman’s research. He explains that ‘exploring’ refers to the determination of the three-dimensional structures of proteins and enzymes. ‘Exploiting’ involves the use of these enzymes to convert substrates into products of value, and ‘evolving’ pertains to mutating the DNA to change the protein, giving it different functions, activities, selectivity, or specificities.

In his lecture, he remarked that if we know the structures of these proteins and enzymes, we can explore what to do with them and how to change them. According to him, there are the unknown knowns, the unknown unknowns, and the known unknowns. “We may know of specific activities and reactions by microorganisms, but we don’t know which enzyme is responsible; similarly, we can know the reactivity of an enzyme, but not necessarily their true physiological functions. I am trying to figure out all these unknowns,” he said.

In his lecture, he also raised the question of whether AI could replace experimental determination of protein structures. "No, not yet; it is only predictions," he believes, commenting that navigating the unknown unknowns is a dangerous place in science.

Establishing the field of structural biology

Prof Opperman, born and raised in the Free State, completed his undergraduate studies at the UFS. Later, in 2008, he obtained his PhD in Biochemistry from the same university. Following his doctoral studies, he conducted postdoctoral research on directed evolution under the guidance of Prof Manfred T Reetz at the Max Planck Institute for Coal Research in Germany, one of the world’s top institutions.

In 2010, he was appointed to the Department of Microbiology and Biochemistry at the UFS, where he has since established the field of structural biology, setting up the infrastructure essential for the advancement thereof. This includes equipment, techniques, and methods for determining the three-dimensional structure of proteins. “It is done using protein crystallisation and then X-ray diffraction,” he explains. Most of these X-ray diffraction experiments are then performed at particle accelerators called synchrotrons, such as Diamond Light Source (UK), which can produce intense X-rays.

His current research explores the interface of evolutionary and structure-function relationships of biocatalysts, with a particular focus on their application in green chemistry. Prof Opperman says that understanding both the structure and the function of an enzyme allows one to manipulate it to perform other functions.

Contributing to the broader goals of sustainable development

One of the projects he is working on highlights the potential for sustainable practices in waste management. Prof Opperman is currently part of a European Research Area Network Cofund partnership on Food Systems and Climate (FOSC), which focuses on developing biocatalysts for upcycling waste. An aspect of this work involves studying enzymes that degrade feathers, thereby converting feather waste into useful products such as fertiliser.

Regarding the contribution of his research to the broader goals of sustainable development and environmental protection, he says that enzymes are the base for biotechnology and the bioeconomy. “They can be sustainably produced, the reactions are environmentally friendly, and the resulting products can be classified as natural. There’s no need to use sources that are not sustainable to extract some of these molecules from,” he explains.

His significant contributions to the field are reflected in more than 50 authored and co-authored papers, some of which are published in prestigious journals such as Science, Nature Communications, and Angewandte Chemie. As an NRF B-rated researcher, his work has received funding from various local and international organisations, including industries such as Sasol and the Global Challenges Research Fund.

News Archive

UFS to investigate implementation of quality-monitoring system for SA food industry

2006-02-07

Some of the guests who attended the workshop were from the left Prof James du Preez (Chairperson: Department of Biotechnology at the UFS); Prof Lodewyk Kock (Head: South African Fryer Oil Initiative (SAFOI) at the UFS)); Mrs Ina Wilken (Chairperson: South African National Consumer Union (SANCU)); Prof Herman van Schalkwyk (Dean: Faculty of Natural and Agricultural Sciences at the UFS) and Mr Joe Hanekom (Managing Director of Agri Inspec).
Photo: Stephen Collet

UFS to investigate implementation of quality-monitoring system for SA food industry

The University of the Free State (UFS) will be investigating the implementation of a quality-monitoring service for the South African food industry.

This was decided during a workshop to discuss the external quality monitoring in the edible oil industry of South Africa, which was recently held at the UFS.

Major role players in the fast-food sector like Nando's, Spur, Captain
Dorego's, King Pie Holdings, Black Steer Holdings, etc and various oil
distributors like Felda Bridge Africa, Refill Oils, PSS Oils and Ilanga Oils attended
the workshop. Also present was Mrs Ina Wilken, Chairperson of the South African National Consumer Union (SANCU) and key-note speaker of this workshop. She represented the consumer.

These role players all pledged their support to the implementation of this quality- monitoring system for the whole food industry.

The decision to implement this system follows the various malpractices reported in the press and on TV concerning food adulteration (eg the recent Sudan Red Scare), misrepresentation (eg olive oil scandal exposed in 2001) and the misuse of edible frying oils by the fast-food sector.

“One of the basic rights of consumers is the right to safe food. Consumers must be protected against foods and food production processes which are hazardous to their health. Sufficient guarantee of the safety of all food products and food production processes should be implemented. It does not help to have adequate food standards and legislation and there is no manpower to do the necessary investigation or monitoring,” said Mrs Wilken.

The South African Fryer Oil Initiative (SAFOI), under the auspices of the UFS Department of Microbial, Biochemical and Food Biotechnology, currently monitors edible oils in the food industry and makes a seal of quality available to food distributors.

“Last week’s decision to implement the quality-monitoring system implies that we will now be involving also other departments in the UFS Faculty of Natural and Agricultural Sciences who are involved in various aspects of the food chain in an endeavor to implement this quality monitoring system,” said Prof Herman van Schalkwyk, Dean: Faculty of Natural and Agricultural Sciences at the UFS and one of the main speakers at the workshop.

Prof van Schalkwyk said that the main aim of such a system will be to improve the competitiveness of the South African food industry. “It is clear that the role players attending the workshop are serious about consumer service and that they agree that fraudulent practice should be monitored and corrected as far as possible. Although some of the food outlets have the capacity to monitor the quality of their food, it may not seem to the consumer that this is an objective process. The proposed external monitoring system would counteract this perception amongst consumers,” said Prof van Schalkwyk.

The workshop was also attended by representatives from SAFOI and Agri Inspec, a forensic investigation company collaborating with inter-state and government structures to combat fraud and international trade irregularities.

Agri Inspec has been working closely with SAFOI for a number of years to test the content of edible oils and fats. “Extensive monitoring and control actions have been executed in the edible oil industry during the past four years to ensure that the content and labeling of oil products are correct. Four years ago almost 90% of the samples taken indicated that the content differed from what is indicated on the label. This has changed and the test results currently show that 90% of the products tested are in order. However, to maintain this quality standard, it is necessary that quality monitoring and educational campaigns are continuously performed,” said Mr Joe Hanekom, Managing Director of Agri Inspec.

“The seal of quality presented by SAFOI should also be extended to include all the smaller oil containers used by households,” Mrs Wilken said.

The SAFOI seal of quality is currently displayed mainly on some oil brands packed in bigger 20 liter containers, which include sunflower oil, cottonseed oil, palm oil etc which are used by restaurants and fast food outlets. “Any oil type is eligible to display the seal when meeting certain standards of authenticity. In order to display the seal, the distributor must send a sample of each oil batch they receive from the manufacturer to SAFOI for testing for authenticity, ie that the container’s content matches the oil type described on the label. This is again double checked by Agri Inspec, which also draws samples countrywide from these certified brands from the end-user (restaurant or fast food outlets). If in breach, the seal must be removed from the faulty containers,” said Prof Lodewyk Kock, Head of SAFOI.

“It should however be taken into account that oils without a seal of quality from the UFS can still be of high quality and authentic. Other external laboratories equipped to perform effective authenticity tests may also be used in this respect,” said Prof Kock.

“It is also important to realise that any oil type of quality such as sunflower oil, cottonseed oil, palm oil etc can be used with great success in well controlled frying processes,” he said.

Further discussions will also be held with the Department of Health, the SA National Consumer Union and Agri Inspec to determine priority areas and to develop the most effective low-cost monitoring system.

More information on the UFS oil seal of quality and oil use can be obtained at www.uovs.ac.za/myoilguide

Media release
Issued by: Lacea Loader
Media Representative
Tel: (051) 401-2584
Cell: 083 645 2454
E-mail: loaderl.stg@mail.uovs.ac.za
6 February 2006

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