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29 October 2024 | Story Leonie Bolleurs | Photo Supplied
Thandi Mazibuko
Thandi Mazibuko, with her presentation: LED there be light, was the overall institutional winner in the PhD category and the runner-up in the national competition of this year’s 3MT competition.

The Centre for Graduate Support (CGS) recently (11 October 2024) hosted the annual institutional Three-Minute Thesis Competition (3MT), which was followed by the national competition (25 October). This year, the nationals took place on the UFS Bloemfontein Campus. According to Tshepiso Mokoena, responsible for Research Capacity Development in CGS, the participating master’s and PhD students gave well-prepared presentations. The competition aims to equip postgraduate students with valuable communication and presentation skills.

She says that postgraduate students are encouraged to do research that will benefit the community. “To do this, students should be able to communicate and present their research to a non-specialist audience. The 3MT competition trains and equips them with skills that they will use in their community and workplace,” she noted.

Overall PhD winner

The overall winner in the PhD category of the UFS competition was Thandi Mazibuko with her presentation: LED there be light. Thandi was also announced as the first runner-up at the national competition.

Growing up in Qwaqwa, Thandi’s passion for mathematics and the natural sciences led her to pursue a BSc Physics degree at the UFS in 2013, followed by an honours at the UFS. She then completed her MSc at the University of the Western Cape and worked as a science engagement intern at iThemba LABS in Cape Town, which inspired her to start a YouTube channel with more than 4 800 subscribers, called Thandisayensi. On this channel she uploads Physical Sciences videos for learners in grades 10-12.

Thandi states that she loves learning and being in learning environments; in 2022, she registered for a PhD in Solid State Physics under the supervision of Prof Hendrik Swart and Prof David Motaung.

Her research focuses on synthesising a phosphor material capable of emitting red, green, and blue light, which, when combined, creates the perception of white light. Thandi compared the research process to cooking, explaining how the preparation of phosphors resembles food preparation. She believes that relatable language, analogies, and storytelling are important tools in science communication.

Thandi says that this competition was a valuable platform to improve her science communication skills. “It is an interesting challenge to explain your work in 180 seconds to an audience with different backgrounds,” she said, adding that she is excited to represent the UFS at the national competition.

The other winners

Each department hosts its own 3MT competition, and the winners and runners-up in both the master’s and PhD categories then represent their faculty in the institutional competition.

The master’s category winners from other faculties were:

  • Faculty of Economic and Management Sciences: Evodia Mohoanyane with Does SI/tutoring work and what about it works? Evodia was also the overall winner in the institutional competition in the master’s category.
  • The Humanities: Yonwaba Matshobotiyana with Of Speaking and Visibility: Black Women Poets' Voices in South Africa
  • Health Sciences: Viwe Fokazi with Establishing a novel 3D doxorubicin-resistant triple-negative breast cancer spheroid model

In the PhD category, the winners were:

  • Economic and Management Sciences: Chrizaan Grobbelaar with The use of gamification to enhance retirement preparedness of millennials
  • The Humanities: Sheree Pretorius with The Psychometric Properties of the Prison Adjustment Questionnaire (PAQ) among South African Male Incarcerated Offenders

With Thandi, first runner-up of the institutional competition, Chrizaan, participated in the national 3MT competition. Universities such as the Nelson Mandela University, UNISA, University of KwaZulu-Natal, University of the Western Cape, University of Johannesburg, and the Central University of Technology were also present. 

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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