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08 April 2025 | Story Precious Shamase | Photo Kaleidoscope Studios
Sellwane Moloi
Sellwane Jeanette Moloi celebrates graduating with a PhD in plant stress biology, contributing vital research toward global food security through drought-tolerant wheat studies.

Embarking on a PhD is a transformative journey, one filled with intellectual challenges, personal growth, and the potential to contribute meaningfully to one's field. For Sellwane Jeanette Moloi – who has graduated with her PhD on Friday 4 April during the afternoon session – the path was illuminated by a deep fascination with plants and a determination to address the critical issue of global food security.

 

A spark of curiosity

"The initial spark came from a deep curiosity about plants," Moloi shared. "I was intrigued by how plants function and the key mechanisms that help them survive when exposed to various environmental stress factors. Learning different techniques used to study and analyse plant responses to environmental factors made me even more intrigued." This curiosity fuelled a rigorous exploration into plant stress biology, focusing on how wheat thrives under drought conditions.

 

Navigating the challenges

Reflecting on the experience, however, the PhD journey was not without its hurdles. "One of the most challenging aspects was staying motivated and managing pressure," Moloi confessed. "Setbacks and slow progress can also be overwhelming." Overcoming these obstacles required a strategic approach: "I had to break down larger tasks into manageable tasks and also seek support from my supervisors."

Doubts inevitably arose, especially when experiments did not go as planned. "There were moments when I asked myself what I had actually gotten myself into," she admitted. However, the unwavering support of supervisors, colleagues, friends, and family provided the necessary reassurance. "They always assured me things would work out; I needed to hang in there."

 

Key discoveries and achievements

Amid the challenges, significant discoveries were made. Notably, Moloi found that a drought-tolerant wheat variety suppressed photosynthesis as a potential strategy to avert oxidative stress damage, maintaining cell membrane integrity during stressful conditions. This unexpected turn stressed the complexity of plant survival mechanisms.

Moloi’s dedication and hard work culminated in significant achievements, including winning the best PhD poster in plant physiology at the 46th South Africa Association of Botanists Conference in 2020, 3rd Prize at the University of Johannesburg Postgraduate Symposium in 2023 and publishing two research articles in high-impact journals. These accomplishments are a testament to her research prowess and contribution to the field.

 

Developing essential skills

Beyond the research itself, the PhD journey fostered critical skills vital for future success. ‘Critical thinking, logical reasoning, and problem-solving’ were highlighted as key takeaways. "As a PhD student, your mind is constantly engaged in critically analysing results and providing logical reasoning. I believe these skills are valuable in tackling certain situations and providing solutions to problems."

 

Balancing life and research

Balancing research with other aspects of life proved challenging. "That was challenging, but I created a schedule that worked for me," the graduand explained. "This included dedicating certain days (weekends or holidays) or hours to specific tasks, such as lab work or writing. One thing I would advise a PhD student is that they should make time to rest to avoid burnout."


Contribution to food security

Moloi's research holds significant implications for global food security, a pressing issue in the face of climate change and a growing population. "I hope my research will provide insight into how wheat as a cereal crop thrives under drought stress conditions, and the information can be used by plant breeding initiatives in developing more drought-tolerant crops," she stated. "The opportunity to contribute to the field of plant stress biology – my research focuses on understanding plant response mechanisms under drought, and acquiring such information can address issues related to global food security."


Advice for future PhD candidates

For students embarking on their PhD journey, Moloi offered valuable advice: "This degree requires one to be highly motivated and ask for help whenever there are setbacks. There will always be setbacks in research; you need to go back to the drawing board and look for solutions." She also emphasised the importance of seeking support for mental and emotional well-being. "It is okay to feel overwhelmed; the work can be too much at times. Students need to ask for help. We have professionals on campus who can assist with mental or emotional issues."

Furthermore, Moloi shared personal lessons: "Time management and prioritising important tasks. I also learned that it is important to allow yourself to rest. This degree can affect your mental or emotional health." And a candid reflection: "I did not have much of a social life, and I spent most of my time in the lab."

 

Looking ahead

With a PhD in hand, Moloi aspires to become an expert in her field, collaborating with other researchers and mentoring future students. Her journey exemplifies the dedication, resilience, and intellectual curiosity required to excel in academia and contribute to addressing pressing global challenges.

The university provided essential support throughout her studies, ensuring access to facilities even during the COVID-19 pandemic. "I always had access to the facilities on campus to do my research, even during the COVID-19 pandemic."

Celebrating milestones along the way was crucial, with fellow postgraduates sharing in each other's achievements. "My fellow postgraduates and I often went out for lunch to celebrate everyone's achievements." And the support system was extensive: "Everyone – family, supervisors, friends, and colleagues.”

News Archive

Nuclear Medicine on the forefront of cancer research
2017-07-10

Description: Nuclear Medicine on the forefront of cancer research Tags: Nuclear Medicine, cancer research, Dr Je’nine Horn-Lodewyk’s, tumour detection method, cancer, Department of Nuclear Medicine 

Dr Je’nine Horn-Lodewyk’s tumour detection method
could be the cost-effective breakthrough needed to decrease
the mortality rate in breast cancer patients.
Photo: Anja Aucamp

The field of Nuclear Medicine in South Africa and the rest of the world are expanding rapidly due to the development of hybrid cameras and new radiopharmaceuticals. These developments have a huge impact on the diagnosis and therapy of cancer.

The most advanced of these cameras, Positron emission tomography combined with normal CTs (PETCT), are not yet widely available in South Africa due to the cost of the cameras and the radiopharmaceuticals. A more cost-effective alternative can be of great benefit. To achieve this, the focus should be on developing new radiopharmaceuticals that can be used with the current cost-effective gamma cameras, according to University of the Free State researcher, Dr Je’nine Horn-Lodewyk from the Department of Nuclear Medicine.

Fluorodeoxyglucose (18F-FDG), a radiolabelled glucose analogue, is currently the radiopharmaceutical most commonly used in PET/CT imaging for mainly oncology indications. Although it is considered the gold standard for imaging in several malignancies, it does have certain disadvantages. An 18F-FDG PET/CT diagnostic imaging study can cost between R25 000 and R35 000 for a single patient in the private sector. The 18F-FDG is also more radioactive, which requires much stricter handling and shielding to avoid high radiation dosages to staff and patients.

Successful research potential innovative solution
In the search for the ideal radiopharmaceutical for tumour detection, the South African National Nuclear Energy Corporation (Necsa) developed a local synthesis process for ethylenedicysteine-deoxyglucose (EC-DG). EC-DG is also a glucose analogue similar to FDG. They succeeded in labelling the compound with Technetium-99-metastable-pertechnetate (99mTcO4-), the most common nuclear medicine isotope used for approximately 95% of nuclear medicine procedures, creating 99mTc-EC-DG.

In partnership with Dr Horn-Lodewyk, this compound was successfully used in various animal models and clinical scenarios, resulting in approval by the Medicine Control Council to use it in a human study. Research is also planned in order to investigate diagnostic accuracy in other cancers like lymphoma.  The end result of this research can produce a radiopharmaceutical that is cost effective, does not require the use of costly specialised equipment, has no significant side-effects, no special patient preparation, renders late imaging possible, and has decreased radiation risks.

Dr Horn-Lodewyk is grateful for the support of her mentor, Prof Anton Otto, as well as Dr Gert Engelbrecht, Head of the Department of Nuclear Medicine, Prof Jan Rijn Zeevaart from North-West University’s Preclinical Drug Development Platform and Necsa, and Judith Wagener from Necsa. This innovative research would also not have been possible without the financial assistance of Dr Glen Taylor and Eleanor van der Westhuizen in the Directorate of Research Development.

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