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02 January 2025 | Story Gerda-Marie van Rooyen | Photo Supplied
Prof Linus Franke
Leading the research in South Africa is Prof Linus Franke from the Department of Soil, Crop and Climate Sciences.

Scientists are actively pursuing the successful breeding of diploid hybrid potatoes from inbred lines. This is expected to revolutionise potato breeding as it holds the key to rapid genetic progress. It will introduce new varieties for commercialisation through seed. Currently, existing potato variants have a gene that renders self-pollinated seeds infertile.

Prof Linus Franke, an academic in the Department of Soil, Crop and Climate Sciences at the UFS, is leading the research in South Africa. “This technology allows the production of genetically uniform potato seed that is easy to transport and largely disease-free.” He says this differs from conventional breeding whereby only vegetative propagation is possible due to tetraploid varieties in potatoes. It also risks carrying pests and diseases from one generation to the next – leading to the accumulation of pests and diseases with each round of multiplication.

Seed innovation

Prof Franke explains that Solynta BV, a seed company based in the Netherlands that produces potato varieties that can be grown from seed, has included South Africa in their research efforts because it is one of Africa’s largest producers and exporters. Through his academic relationship with Wageningen University and Research, a Dutch institution renowned for its agricultural endeavours and food production, the UFS became involved in researching hybrid potatoes grown from seed.

Diploid seeds containing two sets of chromosomes allow easier gene manipulation to increase predictability and speedier genetic progress. The breeding approach enables the incorporation of tolerance to pests, diseases, abiotic stresses (cold, heat, drought) and other desired genetic traits.

Although Prof Franke is optimistic about this research, he is not blind to disadvantages. “Potato seeds are tiny and have little energy reserves, making it harder to grow potatoes from seed than from tubers.” He says potatoes from seed will take longer to cultivate than tubers, as farmers need to grow plantlets from seeds first, adding six weeks to the growing period. “It is possible that commercial farmers can grow potatoes directly from seed. Alternatively, perhaps more likely, specialised growers will produce tubers of potatoes from seed; these tubers are then sold as seed tubers to other potato farmers, who then continue their normal practices of producing potatoes for the market from tubers.”

Financial benefits

Prof Franke says farmers have reason to get excited. “Seed potatoes will reduce input costs, as varieties with enhanced tolerance to pests and diseases require less pesticides. Planting one hectare of potatoes requires three to four tonnes of potato tubers, but only one 25 g packet of potato seeds.” Since potatoes are a more valuable commodity than maize, this technology might also increase farmers’ income potential.

News Archive

Newly operational sequencing unit in genomics at UFS
2016-09-09

Description: Next Generation Sequencing  Tags: Next Generation Sequencing

Dr Martin Nyaga and his research assistant,
Tshidiso Mogotsi in the Next Generation
Sequencing Laboratory.
Photo: Charl Devenish

The Next Generation Sequencing (NGS) unit at the UFS was established as an interdisciplinary facility under the Directorate for Research Development, Faculty of Health Sciences and Faculty of Natural and Agricultural Sciences.

The aim of the NGS facility is to aid internal and external investigators undertaking studies on Deoxyribonucleic acid (DNA) sequencing, assembly and bioinformatics approaches using the more advanced Illumina MiSeq NGS platform.

The NGS unit became operational in 2016 and is managed by Dr Martin Nyaga and administered through the office of the Dean, Faculty of Health Sciences, under the leadership of Prof Gert Van Zyl. Dr Nyaga has vast experience in microbial genomics, having done his PhD in Molecular Virology.

He has worked and collaborated with globally recognised centres of excellence in Prokaryotic and Eukaryotic genomics, namely the J. Craig Venter Institute and the Laboratory of Viral Metagenomics, Rega Institute, among others.

The unit has undertaken several projects and successfully generated data on bacterial, viral and human genomes. Currently, work is ongoing on bacterial and fungal metagenomics studies through 16S rRNA sequencing.

In addition, the unit is also working on plasmid/insert sequencing and whole genome sequencing of animal and human rotaviruses. The unit has capacity to undertake other kinds of panels like the HLA, Pan-cancer and Tumor 15 sequencing, among others.

Several investigators from the UFS including but not limited to Prof Felicity Burt, Prof Wijnand Swart, Dr Frans O’Neil, Dr Trudi O'Neill, Dr Charlotte Boucher, Dr Marieka Gryzenhout and Dr Kamaldeen Baba are actively in collaboration with the NGS unit.

The unit has also invested in other specialised equipment such as the M220 Focused-ultrasonicator (Covaris), 2100 Bioanalyzer system (Agilent) and the real-time PCR cycler, the Rotor-Gene Q (Qiagen), which both the UFS and external investigators can use for their research.

Investigators working on molecular and related studies are encouraged to engage with Dr Nyaga on how they would like to approach their genomics projects at the UFS NGS unit. 

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