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25 July 2019 | Story Leonie Bolleurs | Photo Leonie Bolleurs
Plant Sciences Congress
At a public seminar, Understanding human evolution through the study of past environments in the Free State, presented by the UFS Department of Plant Sciences, were, from the left, front: Kristen Wroth, Britt Bousman; back: Prof Louis Scott, mentor in the UFS Department of Plant Sciences, and Michael Toffolo.

Florisbad, a thermal spring situated 45 km northwest of Bloemfontein on the edge of a large dry saltpan, is a well-known fossil site that used to be a large lake where giant buffalo, blue wildebeest, and hippos roamed thousands of years ago. Today, this fossil-bearing spring is not only a tourist attraction and a venue for weddings, but also an established quaternary research station that has attracted several palaeo-scientists since the 1930s, following the discovery of a remarkable human cranium and other fauna. 

Studies of past environments 

Three international researchers studying different aspects of archaeology at this and other sites in the Free State, recently presented lectures at the UFS to a multidisciplinary group of academics in plant sciences, geology, geography, and environmental management.

These lectures are part of the ongoing collaboration regarding fossil plant (pollen), fauna, and archaeological studies between the University of the Free State (UFS), the National Museum, and universities abroad.

Florisbad, a key site for understanding the appearance of modern environments as well as modern humans in Southern Africa, is the focus of the investigations of all three visiting scientists, aiming to provide a better understanding of past Free State environments where human evolution has taken place.

Michael Toffolo, a junior research chair from the University of Bordeaux Montaigne in France, focuses on the reconstruction of site-formation processes, palaeo-environments, and ancient human activities based on the study of the micro-morphology of archaeological deposits. He has been working in Southern Africa since 2013. The title of his lecture was: Reconstructing Pleistocene environments in the Free State by looking at the microscopic sedimentary record. 

Fluoride-preserved bones

Florisbad is widely known for the discovery of an archaic modern human skull of c. 260 000 years old. According to Toffolo, the human probably died, and the remains was left at the spring by the hyenas. The bones consequently absorbed fluoride from the spring water, which counteracted decomposition and helped to preserve it. 

Britt Bousman talked about middle and late Pleistocene terraces and archaeology in the Modder River Valley. He has worked in Southern Africa for the past 43 years and started his collaboration with researchers from the UFS and the National Museum in 1985. They have worked together at several sites, investigating palaeo-environments. Bousman teaches Archaeology in the Department of Anthropology at the Texas State University. 

While most scientists study early human records in rock shelters, especially those near the coast where seafood was harvested by prehistoric people, he is one of only a few researchers who studies the evolution of early human behaviour in central South Africa in the context of their activities in the open environment. 

“Rock shelters are good spaces for human behaviour,” says Bousman. He believes, however, that the Modder River area is a better space to study how humans have survived on the land under changing climatic conditions in the long term; for example, how they hunted and slaughtered animals. This can be seen from the many artefacts they left, such as spearheads, scrapers, etc. Interesting animal remains were also found, such as the bones of an extinct giant zebra at the Erfkroon site along the Modder River, with a head measuring 63 cm compared to that of a current zebra, which measures 54 cm. The only complete horn core of an extinct giant wildebeest was also found at the site. 

The first chemists

According to Bousman, technology changed in the Stone Age and included the production of more grinding stones, indicating that humans collected plants and grinded them. Observations of modern plant-collecting activities suggested that not many plant foods needed grinding. Bousman proposes that different plant components may have been grinded for medicinal mixtures, therefore these ancestors may have assumed the role of chemists. 

Kristen Wroth, a postdoctoral researcher in the Geoarchaeology Working Group at the University of Tübingen, Germany, presented a lecture on early human-environment interactions and ancient pyro technology. She uses a suite of micro-archaeological techniques such as phytolith (microscopic plant silica) analysis, micromorphology, and FTIR to understand both human and Neanderthal behaviour and to reconstruct how local environments have changed in space and through time.


News Archive

Nanotechnology breakthrough at UFS
2010-08-19

 Ph.D students, Chantel Swart and Ntsoaki Leeuw


Scientists at the University of the Free State (UFS) made an important breakthrough in the use of nanotechnology in medical and biological research. The UFS team’s research has been accepted for publication by the internationally accredited Canadian Journal of Microbiology.

The UFS study dissected yeast cells exposed to over-used cooking oil by peeling microscopically thin layers off the yeast cells through the use of nanotechnology.

The yeast cells were enlarged thousands of times to study what was going on inside the cells, whilst at the same time establishing the chemical elements the cells are composed of. This was done by making microscopically small surgical incisions into the cell walls.

This groundbreaking research opens up a host of new uses for nanotechnology, as it was the first study ever in which biological cells were surgically manipulated and at the same time elemental analysis performed through nanotechnology. According to Prof. Lodewyk Kock, head of the Division Lipid Biotechnology at the UFS, the study has far reaching implications for biological and medical research.

The research was the result of collaboration between the Department of Microbial, Biochemical and Food Biotechnology, the Department of Physics (under the leadership of Prof. Hendrik Swart) and the Centre for Microscopy (under the leadership of Prof.Pieter van Wyk).

Two Ph.D. students, Chantel Swart and Ntsoaki Leeuw, overseen by professors Kock and Van Wyk, managed to successfully prepare yeast that was exposed to over-used cooking oil (used for deep frying of food) for this first ever method of nanotechnological research.

According to Prof. Kock, a single yeast cell is approximately 5 micrometres long. “A micrometre is one millionth of a metre – in laymen’s terms, even less than the diameter of a single hair – and completely invisible to the human eye.”

Through the use of nanotechnology, the chemical composition of the surface of the yeast cells could be established by making a surgical incision into the surface. The cells could be peeled off in layers of approximately three (3) nanometres at a time to establish the effect of the oil on the yeast cell’s composition. A nanometre is one thousandth of a micrometre.

Each cell was enlarged by between 40 000 and 50 000 times. This was done by using the Department of Physics’ PHI700 Scanning Auger Nanoprobe linked to a Scanning Electron Microscope and Argon-etching. Under the guidance of Prof. Swart, Mss. Swart en Leeuw could dissect the surfaces of yeast cells exposed to over-used cooking oil. 

The study noted wart like outgrowths - some only a few nanometres in diameter – on the cell surfaces. Research concluded that these outgrowths were caused by the oil. The exposure to the oil also drastically hampered the growth of the yeast cells. (See figure 1)  

Researchers worldwide have warned about the over-usage of cooking oil for deep frying of food, as it can be linked to the cause of diseases like cancer. The over-usage of cooking oil in the preparation of food is therefore strictly regulated by laws worldwide.

The UFS-research doesn’t only show that over-used cooking oil is harmful to micro-organisms like yeast, but also suggests how nanotechnology can be used in biological and medical research on, amongst others, cancer cells.

 

Figure 1. Yeast cells exposed to over-used cooking oil. Wart like protuberances/ outgrowths (WP) is clearly visible on the surfaces of the elongated yeast cells. With the use of nanotechnology, it is possible to peel off the warts – some with a diameter of only a few nanometres – in layers only a few nanometres thick. At the same time, the 3D-structure of the warts as well as its chemical composition can be established.  

Media Release
Issued by: Mangaliso Radebe
Assistant Director: Media Liaison
Tel: 051 401 2828
Cell: 078 460 3320
E-mail: radebemt@ufs.ac.za  
18 August 2010
 

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