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25 July 2019 | Story Leonie Bolleurs | Photo Leonie Bolleurs
Plant Sciences Congress
At a public seminar, Understanding human evolution through the study of past environments in the Free State, presented by the UFS Department of Plant Sciences, were, from the left, front: Kristen Wroth, Britt Bousman; back: Prof Louis Scott, mentor in the UFS Department of Plant Sciences, and Michael Toffolo.

Florisbad, a thermal spring situated 45 km northwest of Bloemfontein on the edge of a large dry saltpan, is a well-known fossil site that used to be a large lake where giant buffalo, blue wildebeest, and hippos roamed thousands of years ago. Today, this fossil-bearing spring is not only a tourist attraction and a venue for weddings, but also an established quaternary research station that has attracted several palaeo-scientists since the 1930s, following the discovery of a remarkable human cranium and other fauna. 

Studies of past environments 

Three international researchers studying different aspects of archaeology at this and other sites in the Free State, recently presented lectures at the UFS to a multidisciplinary group of academics in plant sciences, geology, geography, and environmental management.

These lectures are part of the ongoing collaboration regarding fossil plant (pollen), fauna, and archaeological studies between the University of the Free State (UFS), the National Museum, and universities abroad.

Florisbad, a key site for understanding the appearance of modern environments as well as modern humans in Southern Africa, is the focus of the investigations of all three visiting scientists, aiming to provide a better understanding of past Free State environments where human evolution has taken place.

Michael Toffolo, a junior research chair from the University of Bordeaux Montaigne in France, focuses on the reconstruction of site-formation processes, palaeo-environments, and ancient human activities based on the study of the micro-morphology of archaeological deposits. He has been working in Southern Africa since 2013. The title of his lecture was: Reconstructing Pleistocene environments in the Free State by looking at the microscopic sedimentary record. 

Fluoride-preserved bones

Florisbad is widely known for the discovery of an archaic modern human skull of c. 260 000 years old. According to Toffolo, the human probably died, and the remains was left at the spring by the hyenas. The bones consequently absorbed fluoride from the spring water, which counteracted decomposition and helped to preserve it. 

Britt Bousman talked about middle and late Pleistocene terraces and archaeology in the Modder River Valley. He has worked in Southern Africa for the past 43 years and started his collaboration with researchers from the UFS and the National Museum in 1985. They have worked together at several sites, investigating palaeo-environments. Bousman teaches Archaeology in the Department of Anthropology at the Texas State University. 

While most scientists study early human records in rock shelters, especially those near the coast where seafood was harvested by prehistoric people, he is one of only a few researchers who studies the evolution of early human behaviour in central South Africa in the context of their activities in the open environment. 

“Rock shelters are good spaces for human behaviour,” says Bousman. He believes, however, that the Modder River area is a better space to study how humans have survived on the land under changing climatic conditions in the long term; for example, how they hunted and slaughtered animals. This can be seen from the many artefacts they left, such as spearheads, scrapers, etc. Interesting animal remains were also found, such as the bones of an extinct giant zebra at the Erfkroon site along the Modder River, with a head measuring 63 cm compared to that of a current zebra, which measures 54 cm. The only complete horn core of an extinct giant wildebeest was also found at the site. 

The first chemists

According to Bousman, technology changed in the Stone Age and included the production of more grinding stones, indicating that humans collected plants and grinded them. Observations of modern plant-collecting activities suggested that not many plant foods needed grinding. Bousman proposes that different plant components may have been grinded for medicinal mixtures, therefore these ancestors may have assumed the role of chemists. 

Kristen Wroth, a postdoctoral researcher in the Geoarchaeology Working Group at the University of Tübingen, Germany, presented a lecture on early human-environment interactions and ancient pyro technology. She uses a suite of micro-archaeological techniques such as phytolith (microscopic plant silica) analysis, micromorphology, and FTIR to understand both human and Neanderthal behaviour and to reconstruct how local environments have changed in space and through time.


News Archive

Researcher part of project aimed at producing third-generation biofuels from microalgae in Germany
2016-05-09

Description: Novagreen bioreactor  Tags: Novagreen bioreactor

Some of the researchers and technicians among the tubes of the Novagreen bioreactor (Prof Grobbelaar on left)

A researcher from the University of the Free State (UFS), Prof Johan Grobbelaar, was invited to join a group of scientists recently at the Institute for Bio- and Geo-Sciences of the Research Centre Jülich, in Germany, where microalgae are used for lipid (oil) production, and then converted to kerosene for the aviation industry.

The project is probably the first of its kind to address bio-fuel production from microalgae on such a large scale.  

“The potential of algae as a fuel source is undisputed, because it was these photoautotrophic micro-organisms that were fixing sunlight energy into lipids for millions of years, generating the petroleum reserves that modern human civilisation uses today.  However, these reserves are finite, so the challenge is marrying biology with technology to produce economically-competitive fuels without harming the environment and compromising our food security.  The fundamental ability that microalgae have to produce energy-rich biomass from CO2, nutrients, and sunlight through photosynthesis for biofuels, is commonly referred to as the Third-Generation Biofuels (3G),” said Prof Grobbelaar.

The key compounds used for bio-diesel and kerosene production are the lipids and, more particularly, the triacylglyserols commonly referred to as TAGs.  These lipids, once extracted, need to be trans-esterified for biodiesel, while a further “cracking” step is required to produce kerosene.  Microalgae can store energy as lipids and/or carbohydrates. However, for biofuels, microalgae with high TAG contents are required.  A number of such algae have been isolated, and lipid contents of up to 60% have been achieved.

According to Prof Grobbelaar, the challenge is large-scale, high-volume production, since it is easy to manipulate growth conditions in the laboratory for experimental purposes.  

The AUFWIND project (AUFWIND, a German term for up-current, or new impetus) in Germany consists of three different commercially-available photobioreactor types, which are being compared for lipid production.

Description: Lipid rich chlorella Tags: Lipid rich chlorella

Manipulated Chlorella with high lipid contents (yellow) in the Novagreen bioreactor

The photobioreactors each occupies 500 m2 of land surface area, are situated next to one another, and can be monitored continuously.  The three systems are from Novagreen, IGV, and Phytolutions.  The Novagreen photobioreactor is housed in a glass house, and consist of interconnected vertical plastic tubes roughly 150 mm in diameter. The Phytolutions system is outdoors, and consists of curtains of vertical plastic tubes with a diameter of about 90 mm.  The most ambitious photobioreactor is from IGV, and consists of horizontally-layered nets housed in a plastic growth hall, where the algae are sprayed over the nets, and allowed to grow while dripping from one net to the next.

Prof Grobbelaar’s main task was to manipulate growth conditions in such a way that the microalgae converted their stored energy into lipids, and to establish protocols to run the various photobioreactors. This was accomplished in just over two months of intensive experimentation, and included modifications to the designs of the photobioreactors, the microalgal strain selection, and the replacement of the nutrient broth with a so-called balanced one.

Prof Grobbelaar has no illusions regarding the economic feasibility of the project.  However, with continued research, optimisation, and utilisation of waste resources, it is highly likely that the first long-haul flights using microalgal-derived kerosene will be possible in the not-too-distant future.

Prof Grobbelaar from the Department of Plant Sciences, although partly retired, still serves on the editorial boards of several journals. He is also involved with the examining of PhDs, many of them from abroad.  In addition, he assisted the Technology Innovation Agency of South Africa in the formulation of an algae-biotechnology and training centre.  “The chances are good that such a centre will be established in Upington, in the Northern Cape,” Prof Grobbelaar said.

 

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