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31 March 2020 | Story Leonie Bolleurs | Photo Gerhard de Jager
Prof Linda Basson recently returned from a three-month research expedition in Antarctica. Here she is, relaxing on the ice with the ever-inquisitive Adelie penguins having a gander at these strangers in their snowy world.
Prof Linda Basson recently returned from a three-month research expedition in Antarctica. Here she is, relaxing on the ice with the ever-inquisitive Adelie penguins having a gander at these strangers in their snowy world.

Very little is known about the parasites of Antarctica, which is a highly productive part of the oceans. These small organisms can be used very successfully to determine the health of this fragile ecosystem.

“Our research data can make significant contributions to the biodiversity of parasites, for a start. The data can also be very valuable to indicate the overall health of this large ecosystem – an ecosystem that drives many of the life-giving processes on our planet.” This is the belief of Prof Linda Basson from the Department of Zoology and Entomology at the University of the Free State (UFS). 

She is an aquatic parasitologist who concentrates on various parasites from a wide range of hosts, including vertebrates (fish and amphibians) and invertebrates (plankton, urchins, starfish, sea cucumbers and red-bait).

Prof Basson, together with PhD student Gerhard de Jager, was invited by Prof Isabelle Ansorge, Head of the School of Oceanography, University of Cape Town, to join her research team on the South African National Antarctic Programme (SANAE) Voyage 59 to Antarctica. 

Widen the scope of research 
“Our aim on this trip was to determine how we can collaborate with the various oceanographers to widen the scope of research normally performed on these voyages, in order to also include parasitological aspects. Once we arrived on the continent of Antarctica, we worked to collect a range of hosts from the Southern Ocean to screen these for the whole array of parasites,” she explains. 

No research of any kind on aquatic parasites has ever been done in Penguin Bukta and Akta Bukta, the specific areas where Prof Basson was based with other scientists and the rest of the Agulhas crew.

She adds: “Our research will contribute to the wider knowledge of parasites in marine environments, but specifically in this area where little to nothing is known.”

A chance of a lifetime 
“Antarctica was literally one of the top research destinations on my bucket list. Travelling to and working in Antarctica is a lifelong dream of mine. It was a chance of a lifetime that I could not miss out on,” says Prof Basson. 

Sharing her experience, she says a typical day on board the SA Agulhas II in Antarctica will start with a cup of good, quality coffee and a look at the prevailing weather on the stern of the ship. 

“One would always be amazed by the beautiful, ceaselessly changing water, the restless sea ice and the impressive ancient ice shelf in very invigorating temperatures, while an ethereal Snow Petrel swirls past and the occasional Adelie penguin comes to gaze and contemplate the presence of this large red structure floating in their habitat and obscuring their view. After tearing yourself away from this, the rest of the day would be spent either in the well-equipped laboratory working through collected samples, or else planning the next exciting collection in the intensely cold water.”

Remarkable journey 
To eternalise memories of this unique experience of almost three months, Prof Basson says that, “One cannot go without a fully charged camera with a large SD card, ready to capture the many facets of this exceedingly fragile but enchanting world of ice and sky, ever changing and all in innumerable shades of white”.

“This truly remarkable journey will forever be associated with a myriad of brilliant highlights.”

Finding it extremely difficult to single out a specific highlight, she listed a long list of memorable events, but as a scientist she will always remember “realising the wealth and cornucopia of microscopic life present in the southernmost of our oceans and seeing this first-hand under the microscope”.

News Archive

Nanotechnology breakthrough at UFS
2010-08-19

 Ph.D students, Chantel Swart and Ntsoaki Leeuw


Scientists at the University of the Free State (UFS) made an important breakthrough in the use of nanotechnology in medical and biological research. The UFS team’s research has been accepted for publication by the internationally accredited Canadian Journal of Microbiology.

The UFS study dissected yeast cells exposed to over-used cooking oil by peeling microscopically thin layers off the yeast cells through the use of nanotechnology.

The yeast cells were enlarged thousands of times to study what was going on inside the cells, whilst at the same time establishing the chemical elements the cells are composed of. This was done by making microscopically small surgical incisions into the cell walls.

This groundbreaking research opens up a host of new uses for nanotechnology, as it was the first study ever in which biological cells were surgically manipulated and at the same time elemental analysis performed through nanotechnology. According to Prof. Lodewyk Kock, head of the Division Lipid Biotechnology at the UFS, the study has far reaching implications for biological and medical research.

The research was the result of collaboration between the Department of Microbial, Biochemical and Food Biotechnology, the Department of Physics (under the leadership of Prof. Hendrik Swart) and the Centre for Microscopy (under the leadership of Prof.Pieter van Wyk).

Two Ph.D. students, Chantel Swart and Ntsoaki Leeuw, overseen by professors Kock and Van Wyk, managed to successfully prepare yeast that was exposed to over-used cooking oil (used for deep frying of food) for this first ever method of nanotechnological research.

According to Prof. Kock, a single yeast cell is approximately 5 micrometres long. “A micrometre is one millionth of a metre – in laymen’s terms, even less than the diameter of a single hair – and completely invisible to the human eye.”

Through the use of nanotechnology, the chemical composition of the surface of the yeast cells could be established by making a surgical incision into the surface. The cells could be peeled off in layers of approximately three (3) nanometres at a time to establish the effect of the oil on the yeast cell’s composition. A nanometre is one thousandth of a micrometre.

Each cell was enlarged by between 40 000 and 50 000 times. This was done by using the Department of Physics’ PHI700 Scanning Auger Nanoprobe linked to a Scanning Electron Microscope and Argon-etching. Under the guidance of Prof. Swart, Mss. Swart en Leeuw could dissect the surfaces of yeast cells exposed to over-used cooking oil. 

The study noted wart like outgrowths - some only a few nanometres in diameter – on the cell surfaces. Research concluded that these outgrowths were caused by the oil. The exposure to the oil also drastically hampered the growth of the yeast cells. (See figure 1)  

Researchers worldwide have warned about the over-usage of cooking oil for deep frying of food, as it can be linked to the cause of diseases like cancer. The over-usage of cooking oil in the preparation of food is therefore strictly regulated by laws worldwide.

The UFS-research doesn’t only show that over-used cooking oil is harmful to micro-organisms like yeast, but also suggests how nanotechnology can be used in biological and medical research on, amongst others, cancer cells.

 

Figure 1. Yeast cells exposed to over-used cooking oil. Wart like protuberances/ outgrowths (WP) is clearly visible on the surfaces of the elongated yeast cells. With the use of nanotechnology, it is possible to peel off the warts – some with a diameter of only a few nanometres – in layers only a few nanometres thick. At the same time, the 3D-structure of the warts as well as its chemical composition can be established.  

Media Release
Issued by: Mangaliso Radebe
Assistant Director: Media Liaison
Tel: 051 401 2828
Cell: 078 460 3320
E-mail: radebemt@ufs.ac.za  
18 August 2010
 

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