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13 November 2020 | Story Leonie Bolleurs | Photo Dr Beanelri Janecke
Five of the multidisciplinary team of researchers and some postgraduate students are determining the depth of soil on the underlying rock layer of the sodic site in the Kruger National Park.

When the Vice-Rector: Research, Prof Corli Witthuhn, invited researchers to apply for funding towards multidisciplinary and interdepartmental projects in 2015, Prof Piet le Roux from the Institute for Groundwater Studies, and the late Dr Fred Kruger from the Centre for Environmental Management took the opportunity and proposed a project to study a catenal ecosystem in the Kruger National Park

According to Dr Beanélri Janecke from the Department of Animal, Wildlife and Grassland Sciences, who led the research team on this project, the team of researchers worked for four years, finding links between the catenal ecosystem (which can be described as a hillslope with different zones forming an environmental gradient from crest to foothill) and processes behind some of its abiotic (non-living) and biotic (living) components. 

Large-scale multidisciplinary research project

All their research on this multidisciplinary project was published in one Special Issue of the Koedoe journal at the end of October 2020. Dr Janecke and Prof Johan van Tol from the Department of Soil, Crop and Climate Sciences were guest editors of this special issue. Dr Llewellyn Foxcroft from SANParks is the Editor-in-Chief of the journal. Other UFS departments involved in this project include the Departments of Genetics; Microbial, Biochemical and Food Biotechnology; Plant Sciences; and Zoology and Entomology.

Dr Janecke says there are 12 articles in this issue and, together with the principal researchers (but excluding postgraduate students), there were 12 authors from the UFS (eight departments and divisions in the Faculty of Natural and Agricultural Sciences) and two from SANParks in associated research collaboration with authors from 14 other institutions. 

This special issue of the Koedoe journal covered research on hydrology, flowpaths, and ground water, including the soil types and properties of different zones on the catena. As stated by Dr Janecke, there are also articles on micro-organisms, fungi, and Fusarium in the soil of the root zone of plants (rhizobiome). 

The environment is degrading fast due to human activities, and there is a dire need for research to look at the bigger picture to find solutions on how to conserve ecosystems and not only smaller parts thereof. – Dr Beanélri Janecke

The journal also comprised articles on the vegetation communities and vegetation structure in the different zones. “Research on how the vegetation recovered post-drought of 2016-2017 and on large and small mammals present on the catena and at the closest waterholes was also published. All of these topics were linked in a discussion article on the catenal ecosystem,” says Dr Janecke.

Multidisciplinary research is not conducted very often on this scale. Many researchers will rather focus on one or a few specific aspects of the ecosystem in a specialist research field. 

SANParks introduced supersite concept

Dr Janecke says scientists from SANParks initiated this supersite concept, where research can be focused on specific areas with similar geology and landscapes in the Kruger National Park to generate multidisciplinary data from separate specialist research fields. 

“Our project went one step further and combined different research fields into one project done on a supersite over the same period. This multidisciplinary project created the opportunity for specialist research fields to be published separately in one special issue, but also to combine the expertise in one project that was summarised in a discussion article.”

She believes that the environment is degrading fast due to human activities, and that there is a dire need for research to look at the bigger picture to find solutions on how to conserve ecosystems and not only smaller parts thereof. “There is a need for a more holistic approach to research, and this special issue provides a framework and basis for similar multidisciplinary studies in future,” states Dr Janecke.

This issue is currently widely marketed on all social platforms of the Koedoe journal and AOSIS Publishers, while a podcast interview is also available at:  https://soundcloud.com/aosis-za/koedoe-interview-podcast-2020 (with permission from Louw Lombaard from AOSIS).

News Archive

UFS study on cell development in top international science journal
2008-09-16

A study from the University of the Free State (UFS) on how the change in the packaging of DNA with cell development influenced the expression of genes, will be published in this week’s early edition of the prestigious international, peer-reviewed science journal, the Proceeding of the National Academy of Sciences of the USA (PNAS).

The PNAS journal has an impact factor of 10, which means that studies published in the journal are, on average, referred to by ten other scientific studies in a two year period. The South African Journal of Science, by comparison, has an impact factor of 0.7.

The UFS study, funded by the Wellcome Trust and the National Research Foundation (NRF), looked at how the change in the packaging of DNA with cell development influenced the expression of genes. It is very relevant to research on stem cells, an area of medicine that studies the possible use of undifferentiated cells to replace damaged tissue.

Prof. Hugh Patterton, of the Department of Microbial, Biochemical and Food Biotechnology at the UFS, who led the study, said: "We are extremely proud of this study. It was conceived in South Africa, it was performed in South Africa, the data were analysed in South Africa, and it was published from South Africa."

When a gene is expressed, the information encoded in the gene is used to manufacture a specific protein. In eukaryotes, which include humans, there is approximately 1m of DNA, containing the genes, in every cell. This length of DNA has to fit into a cell nucleus with a diameter of only about 10 micrometer. In order to fit the DNA into such a small volume, eukaryotic cells wrap their DNA onto successive protein balls, termed nucleosomes. Strings of nucleosomes, resembling a bead of pearls, is folded into a helix to form a chromatin fiber. The study from the UFS investigated how the binding of a specific protein, termed a linker histone, that binds to the length of DNA between nucleosomes, influenced the formation of the chromatin fiber and also the activity of genes.

"We found that the linker histone bound to chromatin in yeast, which we use as a model eukaryote, under conditions where virtually all the genes in the organism were inactive. It was widely believed that the binding of the linker histone caused the inactivation of genes. We studied the relationship between the amount of linker histone bound in the vicinity of each gene and the expression of that gene for all the genes in yeast, using genomic techniques. We made the surprising discovery that even through the linker histone preferentially bound to genes under conditions where the genes were shut off, this inactivation of genes was not caused by the binding of the linker histone and folding of the chromatin,” said Prof. Patterton.

He said: “Instead our data strongly suggested that the observed anti-correlation was due to the movement of enzymes along the DNA molecule, involved in processing the information in genes for the eventual manufacture of proteins. This movement of enzymes displaced the linker histones from the DNA. This finding now requires a rethink on aspects of how packaging of DNA influences gene activity."

Prof. Patterton said that his research group, using the Facility for Genomics and Proteomics as well as the Bioinformatics Node at the UFS, was currently busy with follow-up studies to understand how other proteins in nucleosomes affected the activities of genes, as well as with projects to understand how chemicals found in red wine and in green tea extended lifespan. "We are certainly having a marvelous time trying to understand the fundamental mechanisms of life, and the UFS is an exciting place to be if one was interested in studying life at the level of molecules," he said.


Media Release
Issued by: Lacea Loader
Assistant Director: Media Liaison
Tel: 051 401 2584
Cell: 083 645 2454
E-mail: loaderl.stg@ufs.ac.za  
18 September 2008
 

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