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13 November 2020 | Story Leonie Bolleurs | Photo Dr Beanelri Janecke
Five of the multidisciplinary team of researchers and some postgraduate students are determining the depth of soil on the underlying rock layer of the sodic site in the Kruger National Park.

When the Vice-Rector: Research, Prof Corli Witthuhn, invited researchers to apply for funding towards multidisciplinary and interdepartmental projects in 2015, Prof Piet le Roux from the Institute for Groundwater Studies, and the late Dr Fred Kruger from the Centre for Environmental Management took the opportunity and proposed a project to study a catenal ecosystem in the Kruger National Park

According to Dr Beanélri Janecke from the Department of Animal, Wildlife and Grassland Sciences, who led the research team on this project, the team of researchers worked for four years, finding links between the catenal ecosystem (which can be described as a hillslope with different zones forming an environmental gradient from crest to foothill) and processes behind some of its abiotic (non-living) and biotic (living) components. 

Large-scale multidisciplinary research project

All their research on this multidisciplinary project was published in one Special Issue of the Koedoe journal at the end of October 2020. Dr Janecke and Prof Johan van Tol from the Department of Soil, Crop and Climate Sciences were guest editors of this special issue. Dr Llewellyn Foxcroft from SANParks is the Editor-in-Chief of the journal. Other UFS departments involved in this project include the Departments of Genetics; Microbial, Biochemical and Food Biotechnology; Plant Sciences; and Zoology and Entomology.

Dr Janecke says there are 12 articles in this issue and, together with the principal researchers (but excluding postgraduate students), there were 12 authors from the UFS (eight departments and divisions in the Faculty of Natural and Agricultural Sciences) and two from SANParks in associated research collaboration with authors from 14 other institutions. 

This special issue of the Koedoe journal covered research on hydrology, flowpaths, and ground water, including the soil types and properties of different zones on the catena. As stated by Dr Janecke, there are also articles on micro-organisms, fungi, and Fusarium in the soil of the root zone of plants (rhizobiome). 

The environment is degrading fast due to human activities, and there is a dire need for research to look at the bigger picture to find solutions on how to conserve ecosystems and not only smaller parts thereof. – Dr Beanélri Janecke

The journal also comprised articles on the vegetation communities and vegetation structure in the different zones. “Research on how the vegetation recovered post-drought of 2016-2017 and on large and small mammals present on the catena and at the closest waterholes was also published. All of these topics were linked in a discussion article on the catenal ecosystem,” says Dr Janecke.

Multidisciplinary research is not conducted very often on this scale. Many researchers will rather focus on one or a few specific aspects of the ecosystem in a specialist research field. 

SANParks introduced supersite concept

Dr Janecke says scientists from SANParks initiated this supersite concept, where research can be focused on specific areas with similar geology and landscapes in the Kruger National Park to generate multidisciplinary data from separate specialist research fields. 

“Our project went one step further and combined different research fields into one project done on a supersite over the same period. This multidisciplinary project created the opportunity for specialist research fields to be published separately in one special issue, but also to combine the expertise in one project that was summarised in a discussion article.”

She believes that the environment is degrading fast due to human activities, and that there is a dire need for research to look at the bigger picture to find solutions on how to conserve ecosystems and not only smaller parts thereof. “There is a need for a more holistic approach to research, and this special issue provides a framework and basis for similar multidisciplinary studies in future,” states Dr Janecke.

This issue is currently widely marketed on all social platforms of the Koedoe journal and AOSIS Publishers, while a podcast interview is also available at:  https://soundcloud.com/aosis-za/koedoe-interview-podcast-2020 (with permission from Louw Lombaard from AOSIS).

News Archive

Dr Charlotte Boucher and Lindi Heyns examine possible anti-microbial activity in the skin of Western olive toad species
2014-12-22

 

Researchers Lindi Heyns and Dr Charlotte Boucher are working together on an interdisciplinary project between the Departments of Zoology and Entomology and Veterinary Biotechnology at the University of the Free State (UFS). The focus of their research is on the preliminary biochemical description of skin secretions in some South African toads.

The project forms part of an Honours study executed by Dwayne Pike under Heyns’ supervision. He is co-supervised by Dr Boucher who is assisting with the biochemical and microbiological assays.

Dr Boucher said, “Amphibians are characterised by the presence of cutaneous glands spread over the skin. There are two types of glands, namely mucous and granular (poison), located on the inner surface of the epidermis. Mucous glands are widely dispersed over the skin, while granular glands can be grouped and enlarged in specific regions. Mucous glands are generally associated with maintenance of humidity and cutaneous respiration, whereas granular glands function in chemical defence against predators and/or microbial infection. Studies indicate that the compounds produced by the granular glands belong to numerous chemical classes with diverse pharmacological activities.”

The products secreted by granular glands are rich in low molecular weight constituents of varied molecular types, including proteins, peptides and toxins. These secretions make the toad foul-tasting to predators and even toxic to other frog species. In addition, amphibians offer an attractive source of novel antimicrobials. Studies indicate that as a response to inhabiting microorganism-rich environments they synthesise and secrete a diverse array of antimicrobial peptides (AMPs) as an innate form of defence. Extensive research by various other research groups has been carried out on antimicrobial peptides of the genus Rana; however, hardly any studies have investigated the antimicrobial activity of African frog species.

The focus of this preliminary project is to determine the protein composition of the glandular secretions of the Western olive toad (Amietophrynus poweri), using biochemical tests, such as SDS-PAGE also known as protein gel electrophoresis combined with mass-spectrometry used to identify unknown peptides and proteins. This will give us an overview of the composition of the glandular secretions. Furthermore, we are also looking at microbiological tests, which include assays that test for possible anti-microbial activity against various bacterial and fungal species.

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