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13 November 2020 | Story Leonie Bolleurs | Photo Dr Beanelri Janecke
Five of the multidisciplinary team of researchers and some postgraduate students are determining the depth of soil on the underlying rock layer of the sodic site in the Kruger National Park.

When the Vice-Rector: Research, Prof Corli Witthuhn, invited researchers to apply for funding towards multidisciplinary and interdepartmental projects in 2015, Prof Piet le Roux from the Institute for Groundwater Studies, and the late Dr Fred Kruger from the Centre for Environmental Management took the opportunity and proposed a project to study a catenal ecosystem in the Kruger National Park

According to Dr Beanélri Janecke from the Department of Animal, Wildlife and Grassland Sciences, who led the research team on this project, the team of researchers worked for four years, finding links between the catenal ecosystem (which can be described as a hillslope with different zones forming an environmental gradient from crest to foothill) and processes behind some of its abiotic (non-living) and biotic (living) components. 

Large-scale multidisciplinary research project

All their research on this multidisciplinary project was published in one Special Issue of the Koedoe journal at the end of October 2020. Dr Janecke and Prof Johan van Tol from the Department of Soil, Crop and Climate Sciences were guest editors of this special issue. Dr Llewellyn Foxcroft from SANParks is the Editor-in-Chief of the journal. Other UFS departments involved in this project include the Departments of Genetics; Microbial, Biochemical and Food Biotechnology; Plant Sciences; and Zoology and Entomology.

Dr Janecke says there are 12 articles in this issue and, together with the principal researchers (but excluding postgraduate students), there were 12 authors from the UFS (eight departments and divisions in the Faculty of Natural and Agricultural Sciences) and two from SANParks in associated research collaboration with authors from 14 other institutions. 

This special issue of the Koedoe journal covered research on hydrology, flowpaths, and ground water, including the soil types and properties of different zones on the catena. As stated by Dr Janecke, there are also articles on micro-organisms, fungi, and Fusarium in the soil of the root zone of plants (rhizobiome). 

The environment is degrading fast due to human activities, and there is a dire need for research to look at the bigger picture to find solutions on how to conserve ecosystems and not only smaller parts thereof. – Dr Beanélri Janecke

The journal also comprised articles on the vegetation communities and vegetation structure in the different zones. “Research on how the vegetation recovered post-drought of 2016-2017 and on large and small mammals present on the catena and at the closest waterholes was also published. All of these topics were linked in a discussion article on the catenal ecosystem,” says Dr Janecke.

Multidisciplinary research is not conducted very often on this scale. Many researchers will rather focus on one or a few specific aspects of the ecosystem in a specialist research field. 

SANParks introduced supersite concept

Dr Janecke says scientists from SANParks initiated this supersite concept, where research can be focused on specific areas with similar geology and landscapes in the Kruger National Park to generate multidisciplinary data from separate specialist research fields. 

“Our project went one step further and combined different research fields into one project done on a supersite over the same period. This multidisciplinary project created the opportunity for specialist research fields to be published separately in one special issue, but also to combine the expertise in one project that was summarised in a discussion article.”

She believes that the environment is degrading fast due to human activities, and that there is a dire need for research to look at the bigger picture to find solutions on how to conserve ecosystems and not only smaller parts thereof. “There is a need for a more holistic approach to research, and this special issue provides a framework and basis for similar multidisciplinary studies in future,” states Dr Janecke.

This issue is currently widely marketed on all social platforms of the Koedoe journal and AOSIS Publishers, while a podcast interview is also available at:  https://soundcloud.com/aosis-za/koedoe-interview-podcast-2020 (with permission from Louw Lombaard from AOSIS).

News Archive

Newly operational sequencing unit in genomics at UFS
2016-09-09

Description: Next Generation Sequencing  Tags: Next Generation Sequencing

Dr Martin Nyaga and his research assistant,
Tshidiso Mogotsi in the Next Generation
Sequencing Laboratory.
Photo: Charl Devenish

The Next Generation Sequencing (NGS) unit at the UFS was established as an interdisciplinary facility under the Directorate for Research Development, Faculty of Health Sciences and Faculty of Natural and Agricultural Sciences.

The aim of the NGS facility is to aid internal and external investigators undertaking studies on Deoxyribonucleic acid (DNA) sequencing, assembly and bioinformatics approaches using the more advanced Illumina MiSeq NGS platform.

The NGS unit became operational in 2016 and is managed by Dr Martin Nyaga and administered through the office of the Dean, Faculty of Health Sciences, under the leadership of Prof Gert Van Zyl. Dr Nyaga has vast experience in microbial genomics, having done his PhD in Molecular Virology.

He has worked and collaborated with globally recognised centres of excellence in Prokaryotic and Eukaryotic genomics, namely the J. Craig Venter Institute and the Laboratory of Viral Metagenomics, Rega Institute, among others.

The unit has undertaken several projects and successfully generated data on bacterial, viral and human genomes. Currently, work is ongoing on bacterial and fungal metagenomics studies through 16S rRNA sequencing.

In addition, the unit is also working on plasmid/insert sequencing and whole genome sequencing of animal and human rotaviruses. The unit has capacity to undertake other kinds of panels like the HLA, Pan-cancer and Tumor 15 sequencing, among others.

Several investigators from the UFS including but not limited to Prof Felicity Burt, Prof Wijnand Swart, Dr Frans O’Neil, Dr Trudi O'Neill, Dr Charlotte Boucher, Dr Marieka Gryzenhout and Dr Kamaldeen Baba are actively in collaboration with the NGS unit.

The unit has also invested in other specialised equipment such as the M220 Focused-ultrasonicator (Covaris), 2100 Bioanalyzer system (Agilent) and the real-time PCR cycler, the Rotor-Gene Q (Qiagen), which both the UFS and external investigators can use for their research.

Investigators working on molecular and related studies are encouraged to engage with Dr Nyaga on how they would like to approach their genomics projects at the UFS NGS unit. 

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