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21 April 2023 | Story Leonie Bolleurs | Photo Supplied
Striving to make a difference in the field of biodiversity conservation, Dr Katlego Mashiane decided to pursue a PhD in Geography, focusing on the spatial modelling of grassland diversity and nutrients in subalpine environments. He received his PhD during the recent April graduation ceremonies on the Qwaqwa Campus.

In the small village of Ga-Mabotia about 25 km outside of Polokwane, Dr Katlego Mashiane grew up, surrounded by rocky mountains characterised by boulder outcrops, where he interacted with nature from an early age. 

He recently obtained his PhD, majoring in Geography, from the University of the Free State (UFS), which was conferred on him during the April graduation ceremonies that took place on the UFS Qwaqwa Campus. The title of his dissertation is Grass nutrients estimation as an Indicator of rangeland quality using satellite remote.

Predicting the presence of biodiversity and nutrients in an area

Based on the principle that diverse grasslands tend to perform better, environmental changes threaten the resilience and services these grassland ecosystems provide. The study examined how many different types of plants and animals can be found at a particular place to enhance our understanding of the ecosystem’s value to humans, and that biodiversity loss will reduce these ecosystem services. Focusing on spatial modelling of grassland diversity, Dr Mashiane specifically investigated the influence of topography and remotely sensed satellite data on species richness and diversity in subalpine environments, and how they are affected by the availability of grass species. To determine this, he used a random forest machine-learning algorithm to find the best information in the data that could be used to estimate the levels of species richness, diversity, and nitrogen in a protected national conservation park. 

His study discovered that some data types – such as the near-infrared variable and certain vegetation data (EVI and SAVI) – were especially useful for determining the number and variety of species in a certain area. With this information, scientists can create models that predict the presence of different types of biodiversity and nutrients in an area.

Playing a key role in protecting our natural assets

Equipped with this knowledge, one will be able to understand how to protect and preserve different types of biodiversity and promote the nutritional value of both plants and animals in the environment. “Land managers could use this information for conservation strategies,” states Dr Mashiane, who decided to pursue this study because he was curious about how environmental changes will affect species.

“Grasslands provide important ecosystem services underpinning human well-being, and therefore warrant our protection; I would like to play a role in protecting our natural assets and contribute to understanding our biomes, especially in the context of global change,” he says.

In the next five years, Dr Mashiane plans to pursue further research and mentor other students in his field of study.

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Dr Charlotte Boucher and Lindi Heyns examine possible anti-microbial activity in the skin of Western olive toad species
2014-12-22

 

Researchers Lindi Heyns and Dr Charlotte Boucher are working together on an interdisciplinary project between the Departments of Zoology and Entomology and Veterinary Biotechnology at the University of the Free State (UFS). The focus of their research is on the preliminary biochemical description of skin secretions in some South African toads.

The project forms part of an Honours study executed by Dwayne Pike under Heyns’ supervision. He is co-supervised by Dr Boucher who is assisting with the biochemical and microbiological assays.

Dr Boucher said, “Amphibians are characterised by the presence of cutaneous glands spread over the skin. There are two types of glands, namely mucous and granular (poison), located on the inner surface of the epidermis. Mucous glands are widely dispersed over the skin, while granular glands can be grouped and enlarged in specific regions. Mucous glands are generally associated with maintenance of humidity and cutaneous respiration, whereas granular glands function in chemical defence against predators and/or microbial infection. Studies indicate that the compounds produced by the granular glands belong to numerous chemical classes with diverse pharmacological activities.”

The products secreted by granular glands are rich in low molecular weight constituents of varied molecular types, including proteins, peptides and toxins. These secretions make the toad foul-tasting to predators and even toxic to other frog species. In addition, amphibians offer an attractive source of novel antimicrobials. Studies indicate that as a response to inhabiting microorganism-rich environments they synthesise and secrete a diverse array of antimicrobial peptides (AMPs) as an innate form of defence. Extensive research by various other research groups has been carried out on antimicrobial peptides of the genus Rana; however, hardly any studies have investigated the antimicrobial activity of African frog species.

The focus of this preliminary project is to determine the protein composition of the glandular secretions of the Western olive toad (Amietophrynus poweri), using biochemical tests, such as SDS-PAGE also known as protein gel electrophoresis combined with mass-spectrometry used to identify unknown peptides and proteins. This will give us an overview of the composition of the glandular secretions. Furthermore, we are also looking at microbiological tests, which include assays that test for possible anti-microbial activity against various bacterial and fungal species.

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