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18 November 2024 | Story Nicolle Loader and Rudi Swart | Photo Supplied
Centre for Mineral Biogeochemistry
The team from the UFS who assisted with the Tritech National Science and Technology Fair. Back, from left, Prof Johan Venter, Dr Ernie Langner, Dr Litheko Nkabiti, Dr Rudi Swart, all from the Department of Chemistry; Vuyelwa Nkoi, Department of Sustainable Food Systems and Development; Dr Mariana Erasmus and Ouma Ngoepe from the CMBG. Front, from left, Khezwo Nematshema, Department of Sustainable Food Systems and Development; Dr Dumisani Kama, Department from Chemistry; Muhammad Sallie, CMBG; Shahiëda Cloete, SFSD; Nicolle Loader, CMBG; and Mellisa Pringle (Lasec)

The Centre for Mineral Biogeochemistry (CMBG) at the University of the Free State’s (UFS) support and partnership of the Tritech National Science and Technology Fair reached new heights this year with the event set to be featured in a new television series.

CMBG has been a proud sponsor and partner of the Tritech National Science and Technology Fair for the past four years. Established in 2008, Tritech is a renowned competition that allows Grade 7 to Grade 12 learners from across South Africa to showcase their research abilities. The Tritech National Science Competition serves as a beacon of opportunity for young minds, fostering their passion for scientific exploration and igniting their curiosity. It is designed to enhance scientific literacy, encourage innovation, and nurture the next generation of scientists and inventors.

“The CMBG has consistently demonstrated its commitment to promoting education and empowering young individuals. As a result, we have partnered with Tritech since 2021, and I believe that this partnership has opened new doors and possibilities for our young scientists. We are incredibly honoured to be part of such a dynamic and inspiring environment,” said Dr Mariana Erasmus, Vice Director: CMBG and Tritech EXCO member.

Power of science and technology

According to Dr Erasmus, at the CMBG, they believe in the power of science and technology to transform our world. Their involvement in this event aligns perfectly with the centre’s commitment to fostering innovation, encouraging critical thinking, and nurturing the next generation of scientists and innovators.

“We are excited about the opportunities Tritech presents to the university to engage with cutting-edge projects, to exchange ideas, and build lasting collaborations. The spirit of curiosity and exploration that Tritech embodies is exactly what drives our institution’s mission forward.”

In addition to assisting with the judging of projects during the National Science Competition at the Merensky Agricultural Academy in Tzaneen, the CMBG presents a fun but intellectually stimulating activity, showcasing various STEM-related disciplines that UFS offers to inspire students from across South Africa to celebrate scientific discovery. Each year, the CMBG collaborates with a different department for these activities, and this year, in October 2024, the collaboration was with the UFS Department of Chemistry.

‘Science Olympics’

The theme for Tritech 2024 was “Science Olympics”, and after the formalities of the project presentations were completed, the fair kicked off with a grand procession led by bearers of flaming torches and flags reminiscent of Olympic ceremonies. “Learners marched proudly, symbolising their commitment to science, with representatives from UFS and Tritech EXCO leading the way, an unforgettable visual display honoured the learners’ passion for exploration,” said Dr Erasmus.

The event transitioned into a video presentation explaining the misuse of performance-enhancing drugs in sports, followed by interactive activities focused on analysing possible doping agents. Thereafter, the UFS team guided learners through hands-on experiments, demonstrating the science behind identifying performance-enhancing drugs. Through engaging, real-world applications, learners were not only able to detect these substances but quantify their findings, making the learning experience both fun and educational.

As the night closed, the Chemistry team mesmerised the audience with a ‘magic show’. Explosions of colour, booming sounds, and flashes of light filled the sky, leaving everyone in awe. The show combined entertainment and chemistry, showcasing the wonders of science through spectacular reactions.

On TV

The fair concluded the next day with activities like kart building and livestock showcases, ending with a prize-giving ceremony to reward the learners for their hard work throughout the year. Adding to the excitement, the event is set to be featured in a new television series, with the pilot episode filmed during the weekend of Tritech Nationals 2024.

“The series promises to bring the Tritech experience to life for a wider audience, aiming to capture the incredible spirit, creativity, and innovation that defines Tritech. It will be an incredible opportunity to gain recognition, inspire others, and highlight the importance of science and technology in solving real-world challenges,” Dr Erasmus concluded.

This year’s fair was a celebration of knowledge, creativity, and the endless possibilities of science, leaving a lasting impact on all who attended.

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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