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17 August 2021 | Story Nonsindiso Qwabe | Photo Supplied
A story of hope, courage, resilience, and working your way to the top. Dilahlwane Mohono started working as a cleaner at the UFS in 1992. Today, she is a Senior Faculty Officer in the Faculty of Natural and Agricultural Sciences and holds two honours degrees.

Fuelled by her deep pain due to falling pregnant during her matric year, as well as the desire to one day hold a qualification, Dilahlwane Mohono – Senior Faculty Officer in the Faculty of Natural and Agricultural Sciences – says her story is her favourite, as it is a story of hope, courage, resilience, and working your way to the top.

Because of her pregnancy, Mohono did not complete Grade 12 in 1990. She got married immediately thereafter. In 1992, she began working as a cleaner for a company that was insourced to provide meals on the University of the Free State Qwaqwa Campus. She says this experience often left her with mixed feelings, as it hurt to see her peers studying further, but this is also what ultimately motivated her to complete her matric. “Graduation ceremonies were the most painful events for me. This eventually pushed me to turn to ABET to complete my matric in 1994. From there, I was determined to start my academic journey, so I registered with Stanford Business College for a Secretariat and Computer Skills Diploma. It was a six-month course, but I graduated in the end. That graduation inspired me. It motivated me to study further. Juggling work and studying became challenging, because I was a young mother and wife, but I had to attend classes after work.”

The joy of education

In 2000, she registered for a Higher Diploma with Unisa, and at the same time took up a new position in the library on campus. At the time, it was still called the University of the North Qwaqwa Campus. She recalls this as the beginning of her upward trajectory in life. “The joy of education and seeing how far I have come made me realise how much potential I have, so in 2003 I registered for a BA Sociology and English degree at the UFS, and thereafter went on to do my BA Honours in Sociology.”

She took up a new position as Assistant Officer in the Faculty of Education. Wishing to be relevant to her new faculty, Mohono completed a Postgraduate Certificate in Education (PGCE), and thereafter a second honours degree in Curriculum Studies. “I felt like I was flowing. My colleagues helped me out and I wanted to ensure that I was the best in everything, because I did not want to disappoint them. What always encouraged me, were my children. I separated from my husband in 2003, so I found myself raising my kids single-handedly, and I needed to show them that we could all make it, and life would still go on.”

In 2018, the Faculty of Natural and Agricultural Sciences advertised a senior position. Mohono says she took a chance and applied because she wanted more growth. “I am now a Senior Faculty Officer. I was privileged to be afforded an opportunity to work for the university, so I always thought – how can I work in an academic higher learning environment and not use the opportunity to study and pursue an education.”

Is there a woman who inspires you and who you would like to celebrate this Women’s Month, and why?
It has to be my late mother. She was a very hardworking woman, but she passed on very early, in her early 40s. She worked in the same dining hall I started in, and a lot of the women I worked with then worked with her too. They took me under their wings and motivated me to continue chasing my dreams. Some were not even educated themselves, but they motivated me. I drew a lot of inspiration from them.

What advice would you give to the 15-year-old you?

She must take pride in being a woman, know who she is, and know that she must be brave and confident, and run with education as the key to her success. If it wasn’t for education, I shudder to think where I could’ve been. When I look back and see what I have achieved, being able to rise from all that while single-handedly raising my children, it makes me feel very strongly about myself. I am a very powerful woman. 

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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